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Protective immune responses in enteromucosa tissues of cats infected with Toxoplasma gondii

Research Project

Project/Area Number 08660376
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionObihiro University of Agriculture & Veterinary Medicine

Principal Investigator

OMATA Yoshitaka  Obihiro University of Agriculture & Veterinary Medicine, Department of Veterinary Physiology, Assciate Professor, 畜産学部, 助教授 (10132987)

Co-Investigator(Kenkyū-buntansha) SAITO Atsushi  Obihiro University of Agriculture & Veterinary Medicine, Department of Veterinar, 畜産学部, 教授 (10002263)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsToxoplasma gondii / cats / enteroepithelial tissues / Interleukin 12 / IgA / protective immunity / stage of Gametogony / lgA
Research Abstract

1) Identification of interleukin 12 in various tissue organs of cats infected with Toxoplasma gondii ; Interleukin 12 -35kD and -40 kD subunits of cats were identified by RT-PCR.In the spleen and mesenteric lymphnodes of cats reinfected with T.gondii, IL-12 40kD subunit was expressed transiently post reinfection.
2) Detection of secretory IgA and its protective roles in enteromucosa tissues of cats ; Anti-T.gondii antibody IgA was detected in feces and intestinal washings ofcats infected with T.gondii. Supplement of the washings in the culture medium suppressed the parasite penetration activity of feline embryonic fibroblasts.
3) Stage-spcific antigens associated with gametogenesis of Toxoplasma gondii ; Enteroepithelial stage parasites were isolated by Percoll density gradient centrifugation. Enteroepithelial stage parasite specific antigens were detected by antisera of mice and cats immunized with the parasites.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Yoshitaka, OMATA, et al.: "Positive evidence that antil-Toxoplasma gondii IgA antibody exists in the intestinaltract of infected cats and exerts positive activity against the infection" Veterinary Parasitology. 73. 1-11 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshitaka, OMATA, et al.: "Isolation of coccidian enteroepithelial stages of Toxoplasma gondii from the intestinal mucosa of cats by Percoll density gradientcentrifugation" Parasitology Research. 83. 574-577 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshitaka OMATA et al.: "Isolation of coccidian enteroepithelial stages of Toxoplasma gondii from the intestinal mucosa of cats by Percoll density gradient centrifugation." Parasitology Research. 83. 574-577 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshitaka OMATA et al.: "Positive evidence that anti-Toxoplasma gondii IgA antibody exists in the intestinal tract of infected cats and exerts positive activity against the infection." Veterinary Parasitology. 73. 1-11 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshitaka OMATA, et al.: "Isolation of coccidian enteroepithelial stages of Toxoplasma gondii from the intestinal mucosa of cats by Percoll density gradient centrifugation" Parasitology Research. 83. 574-577 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoshitaka OMATA, et al.: "Positive evidence that anti-Toxoplasma gondii IgA antibody exists in the intestinal tract of infected cats and exerts positive activitv against the infection" Veterinary Parasitology. 73. 1-11 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoshitaka OMATA: "Isolation of coccidian enteroepithelial stages of Toxoplasma gondii from the intestinal mucosa of cats by Percoll density gradient centrifugation" Parasitology Research. (印刷中).

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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