Protective immune responses in enteromucosa tissues of cats infected with Toxoplasma gondii

• Project/Area Number: 08660376
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: Obihiro University of Agriculture & Veterinary Medicine
• Principal Investigator: OMATA Yoshitaka Obihiro University of Agriculture & Veterinary Medicine, Department of Veterinary Physiology, Assciate Professor, 畜産学部, 助教授 (10132987)
• Co-Investigator(Kenkyū-buntansha): SAITO Atsushi Obihiro University of Agriculture & Veterinary Medicine, Department of Veterinar, 畜産学部, 教授 (10002263)
• Project Period (FY): 1996 – 1997
• Project Status: Completed (Fiscal Year 1997)
• Budget Amount: ¥2,600,000 (Direct Cost: ¥2,600,000); Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 1996: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: Toxoplasma gondii / cats / enteroepithelial tissues / Interleukin 12 / IgA / protective immunity / stage of Gametogony / lgA

Research Abstract

1) Identification of interleukin 12 in various tissue organs of cats infected with Toxoplasma gondii ; Interleukin 12 -35kD and -40 kD subunits of cats were identified by RT-PCR.In the spleen and mesenteric lymphnodes of cats reinfected with T.gondii, IL-12 40kD subunit was expressed transiently post reinfection.
2) Detection of secretory IgA and its protective roles in enteromucosa tissues of cats ; Anti-T.gondii antibody IgA was detected in feces and intestinal washings ofcats infected with T.gondii. Supplement of the washings in the culture medium suppressed the parasite penetration activity of feline embryonic fibroblasts.
3) Stage-spcific antigens associated with gametogenesis of Toxoplasma gondii ; Enteroepithelial stage parasites were isolated by Percoll density gradient centrifugation. Enteroepithelial stage parasite specific antigens were detected by antisera of mice and cats immunized with the parasites.

Research Products

• [Publications] Yoshitaka, OMATA, et al.: "Positive evidence that antil-Toxoplasma gondii IgA antibody exists in the intestinaltract of infected cats and exerts positive activity against the infection" Veterinary Parasitology. 73. 1-11 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yoshitaka, OMATA, et al.: "Isolation of coccidian enteroepithelial stages of Toxoplasma gondii from the intestinal mucosa of cats by Percoll density gradientcentrifugation" Parasitology Research. 83. 574-577 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Yoshitaka OMATA et al.: "Isolation of coccidian enteroepithelial stages of Toxoplasma gondii from the intestinal mucosa of cats by Percoll density gradient centrifugation." Parasitology Research. 83. 574-577 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yoshitaka OMATA et al.: "Positive evidence that anti-Toxoplasma gondii IgA antibody exists in the intestinal tract of infected cats and exerts positive activity against the infection." Veterinary Parasitology. 73. 1-11 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Yoshitaka OMATA, et al.: "Isolation of coccidian enteroepithelial stages of Toxoplasma gondii from the intestinal mucosa of cats by Percoll density gradient centrifugation" Parasitology Research. 83. 574-577 (1997)
• [Publications] Yoshitaka OMATA, et al.: "Positive evidence that anti-Toxoplasma gondii IgA antibody exists in the intestinal tract of infected cats and exerts positive activitv against the infection" Veterinary Parasitology. 73. 1-11 (1997)
• [Publications] Yoshitaka OMATA: "Isolation of coccidian enteroepithelial stages of Toxoplasma gondii from the intestinal mucosa of cats by Percoll density gradient centrifugation" Parasitology Research. (印刷中).