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犬バベシア感染にともなう赤血球膜の酸化傷害と貧血

Research Project

Project/Area Number 08660385
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied veterinary science
Research InstitutionMIYAZAKI UNIVERSITY

Principal Investigator

MAKIMURA Susumu  MIYAZAKI UNIVERSITY,FACULTY OF AGRICULTURE,Professor, 農学部, 教授 (80003127)

Co-Investigator(Kenkyū-buntansha) HORII Yoichiro  MIYAZAKI UNIVERSITY,FACULTY OF AGRICULTURE,Professor, 農学部, 教授 (80173623)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsBabesia gibsoni infection / dog / anemia / RBC-bound IgG / commpetitive ELISA / Band 3 / oxidative damage / Babesia gibsoni injection / RBC-bound IgG / oxidative damage / Babesia gibsoni / anepua / RBC-binding IgG / Competitute ELISA
Research Abstract

Anemia is the primary clinical finding manifested by Babesia gibsoni-infected dogs. Significant elevated anti-erythrocyte ELISA levels were demonstrated in sera of infected dogs. To clarify the involvement of anti-erythrocyte IgG antibody in anemia during B.gibsoni infection, a simple method to quantify erythrocyte surface-bound IgG was established and applied to quantification of bound IgG in infected and healthy dogs. Erythrocyte surface-bound IgG value was shown to elevate significantly in clinical infected dogs compared to that of subclinically infected and healthy dogs, moreover the increased level was consistent with Coombs'test results. Serum anti-RBCm antibodies of the dogs before and after the experimental inoculation with parasitized erythrocytes reacted with cytoskeletal and transmembrane structural (band 3) proteins of either self or non-self RBCm. Anti-RBCm antibodies eluted from erythrocytes of B.gibsoni-infected dogs reacted with band 3 and 4.2 of self and non-self RBCm. Anti-RBCm and the surface of RBC treated with phenylhydrazine or neuraminidase, but not to that of non-treated treated RBC.Based from this results, anti-cytoskeletal protein antibody in sera of B.gibsoni-infected dogs is considered to attach to native RBCm, and furthermore free radical-induced oxidative stress imposed on RBC and sialic acid removal from grycoproteins of RBC are considered necessary for anti-transmembrane protein antibody in sera of B.gibsoni-infected dogs to be bound to RBCs. These are important to elucidating the mechanism of the marked in crease in RBCs-bound IgG value and anemia in B.gibsoni infection.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Adachi, K., Nagatomo, H., and Makimura, S: "Quantitation of erythrocyte-bound IgG by commpetitive solid-phase enzymeimmunoassay." J.Vet.Med.Sci.56. 449-502 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Adachi, K., Tateishi, M.and Makimura, S.: "Elevated erythrocyte-bound IgG value in dogs with clinical Babesia gibsoni infection." J.Vet,Med,Sci,. 56. 577-759 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Adachi, K., Shimizu, T.and Makimura, S.: "Reactivity of serum anti-erythrocyte membrane antibody in Babesia gibsoni-infection." J.Vet,Med,Sci,. 56. 997-999 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Adachi, K., Shimizu, T., and Makimura, S.: "Immunologic characteristics of anti-erythrocyte antibody produced during Babesia gibsoni infection." J.Vet,Med,Sci,. 57. 121-123 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Adachi, K., Nagatomo, H., Shimizu, T., Horii, H., and Makimua, S.: "Quantitation of erythrocyte-bound IgG by commpetitive solid-phase enzymeimmunoassay." J.Vet.Med.Sci. 56(3). 499-502 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Adachi, K., Tateishi, M., Horii, Y., Nagatomo, H.Shimizu, T., and Makimura, S.: "Elevated erythrocyte-bound IgG value in dogs with clinical Babesia gibsoni infection." J.Vet.Med.Sci. 56(4). 757-759 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Adachi, K., Tateishi, M., Horii, H., Nagatomo, H.Shimizu, T., and Makimura, S.: "Reactivity of serum anti-erythrocyte membrane antibody in Babesia gibsoni-infection." J.Vet.Med.Sci.56(5). 997-999 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Adachi, K., Horii, H., Tateishi, M., Nagatomo, H.Shimizu, T., and Makimura, S.: "Immunologic characteristics of anti-erythrocyte antibody produced during Babesia gibsoni infection." J.Vet.Med.Sci.57(1). 121-123 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Adachi,K.,Nagatomo,H.,and Makimura,S.: "Quntitation of erythrocyte-bound IgG by commpetitive solid enzyme immunoassay." J.Vet.Med.Sci.56・3. 449-502 (1994)

    • Related Report
      1997 Annual Research Report
  • [Publications] Adachi,K.,Tateishi,M.,and Makimura,S.: "Elevated erythrocyte-bound IgG value in dogs with clinical Babesia gibsoni infection." J.Vet.Med.Sci.56・4. 757-759 (1994)

    • Related Report
      1997 Annual Research Report
  • [Publications] Adachi,K.,Tateishi,M.,and Makimura,S.: "Immunologic Characteristics of anti-erythrocyte membrane antibody produced in dogs during Babesia gibsoni infection." J.Vet.Med.Sci.57・1. 121-123 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] Adachi,K.,Tateishi,M.,and Makimura,S.: "Reactivity of serum anti-erytrocyte membrane antibody in Babesia gibsoni-infected dogs" J.Vet.Med.Sci.56・5. 997-999 (1994)

    • Related Report
      1997 Annual Research Report
  • [Publications] Adachi,K.,Nagatomo,and Makimura,S.: "Quantitation of erythrocyte-bound IgG by commpetitive solid-phase enzyme immunoassay." J.Vet.Med.Sci.56(3). 499-502 (1994)

    • Related Report
      1996 Annual Research Report
  • [Publications] Adachi,K.,Tateishi,M.and Makimura,S.: "Elevated erythrocyte-bound IgG value in dogs with clinical Babesia gibsoni infection." J.Vet.Med.Sci.56(4). 757-759 (1994)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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