| Project/Area Number |
08660410
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | SHIMANE UNIVERSITY |
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Principal Investigator |
KAWAMUKAI Makoto SHIMANE UNIVERSITY,LEFE AND ENVIRONMENTAL SCIENCE,ASSOCIATE PROFESSOR, 生物資源科学部, 助教授 (70186138)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUDA Hideyuki SHIMANE UNIVERSITY,LEFE AND ENVIRONMENTAL SCIENCE,PROFESSOR, 生物資源科学部, 教授 (50032595)
NAKAGAWA Tsuyoshi SHIMANE UNIVERSITY,MOLECULAR INSTITUE OF GENETICS,ASSOCIATE PROFESSOR, 遺伝子実験施設, 助教授 (30202211)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | RAS / cAMP / Signal transduction / fission yeast / CAP / cytoskelton / RAS / cAMP |
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| Research Abstract |
The adenylyl cyclase - associated protein, CAP,was first identified in Saccharomyces cerevisiae, and succeedingly, its homologs were found in many eukaryotes ranging from yeasts to humans. Yeast CAPs have been found to have three functions : the N - terminal domain as the region for binding adenylyl cyclase, the C - terminal domain as an actin binding domain, and the central proline - rich domainas the SH3 (Src Homology 3) binding region. Here, we report the identification of a cDNA and genomic DNA encoding a CAP homolog from mushroom Lentinus edodes. Southern blotting results suggest it to be a single - copy gene. The L.edodes cap gene contains 8 introns and an open reading frame encoding a 518 amino acid protein. The C - terminal domain shares higher homology (39 - 46 % identity) compared with the N - terminal domain (27 - 35 % identity) with yeast CAPs. Expression of the L.edodes CAP in both S.pombe and in S.cerevisiae complemented defects associated with loss of the C - terminal domain function of endogenous CAP.By using yeast two - hybrid assay, we demonstrated the interaction between the L.edodes CAP and S.pombe actin. This result andfunctional complementation test indicate CAP from mushroom is conserved in terms of the C - terminal function of CAP.
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