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Interactions between Schwann cell and axon during reinnervation to the motor endplate

Research Project

Project/Area Number 08670020
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

KAWABUCHI Masaru  Kyushu University, Faculty of Medicine Professor, 医学部, 教授 (80037449)

Co-Investigator(Kenkyū-buntansha) NADA Osami  Kyushu University, School of Health Sciences Professor, 医療技術短期大学部, 教授 (20040727)
NAKAMURA Keiichiro  Kyushu University, Faculty of Medicine Associate Professor, 医学部, 助教授 (20172398)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsNeuromuscular junction / Axonal guidance / Neural regeneration / Aging / Immunohistochemistry / Schwann cell / Extracellular material / Adhesion molecule / 加齡
Research Abstract

Age-related changes of the interactions between the axon, Schwann cell and extracellular material on reinnervation to the motor endplate were examined during the period of I-8 weeks after crush injury of the sciatic nerve with immunonuofluorescence histochemistry. Observations were done by confocal laser scanning microscopy. To define the distribution and co-localization of the structural components of the regenerating nerve fibers, some specific antibodies were used to label axons (neurofilament, PGP 9.5), Schwann cells (S 100), adhesion molecules [neural cell adhesion molecule. (N-CAM)] and the extracellular material (tenascin). In addition, the structural associations between regenerating nerves and acetylcholine receptors (AChR) were also examined. In the aged animal, the main abnormalities in the process of reinnervation consisted of occasional preterminal and terminal axons which either took a distorted course or were damaged. The most obvious changes was the consistency with degeneration of the terminal Schwann cells. In the motor endplate, loci of aneural or abandoned AChR were frequently found particularly late in the reinnervation process. The distribution and continuities of N-CAM-like immunoreactivity around the Schwann cells Were deranged, and the tenascin pathway through which regenerating axons pass was incompletely constructed. These results taken together indicate disturbance in the axonal guidance coupled with incomplete reformation of synapses with a decrease in the effective area of synaptic contact. This may be due to an age-related unsynchronous maturation among the Schwann cells, axon, and extracellular material during the nerve regeneration.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (42 results)

All Other

All Publications (42 results)

  • [Publications] Masaru Kawabuchi: "The effect of aging on the morphological nerve changes during nerve regeneration" Restorative Neruorolgy & Neruoscience. (accepted).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.T.M.S.Islam: "Expression of NOS,P PSA-N-CAM and S100 protein in the granule cell maturation pathway of the adult guinea pig forebrain" Developmental Braine Research. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.T.M.S.Islam: "Olfactory granule cell dispersion in the adult guinea pig forebrain" Brain Research Protocols. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hiroki Tohma: "Three dimensional structure of c-Kit-positive cellular networks in the guinea pig small intestine and colon" Cell and Tissue Research. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kazuho Hirata: "Postnatal development of Schwann cells at neruomuscular junctions,with special rererence to synapse elimination" Journal of Neruocytology. 26. 799-809 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.T.M.S.Islam: "Expression of N-CAM-H ans S100 protein in the rostral migratory stream of the adult guinea pig" Acta Histochemica et Cytochemica. 30. 315-318 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masaru Kawabuchi: "Neuronal PSA-N-CAM immunopositive clusters in the adult guinea pig forebrain" Abstracts Society for Neuroscience 27th Annulal Meeting. 23. 871 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hiroyuki Isayama: "Distribution and co-localization of nitric oxide synthase and arginosuccinate synhthetase in the cat hypothalamus" Archieves of Histology and Cytology. 60. 477-492 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masaru Kawabuchi: "The effect of aging on reinnervation to the motor endplate" Acta Anatomica Japonica. 72. 360 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masaru Kawabuchi: "A morphological study of thymus stromal cells(TEL-2 cell)which play a role in the elimination of doulble positive immature thymocytes by phagocytosis" Anatomical Record. 244. 271-283 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masaru Kawabuchi: "Expression of NOS,NCAM-H,and S100 protein in the granule cell generation of the adult guinea pig olfactory bulb" Abstracts Society for Neuroscience 26th Annulal Meeting. 22. 980 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masaru Kawabuchi: "Morphological features of collateral innervation and polyneuronal innervation in the skeletal muscle of presenile rats" Annals of Anatomy. 177. 251-265 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.T.M.S.Islam, K.Nakamura, T.Seki, A.Kuraoka, P.C.Emson, M.Kawabuchi: "Expression of NOS,PSA-N-CAM and S100 protein in the granule cell migration pathway of the adult guinea pig forebrain." Developmental Brain Research. (in oress).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.T.M.S.Islam, K.Nakamura, T.Seki, A.Kuraoka, P.C.Emson, M.Kawabuchi: "Olfactory granule cell dispersion in the adult guinea pig system" Brain Research Protocols. (in oress).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Kawabuchi, Z.Chongjian, A.T.M.S.Islam, K.Nakamura, O.Nada.: "The effect of aging on the morphological nerve changes during nerve regeeration." Restor.Neurol.Neuroscience. (accepted).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Tohma, K.Nakamura, A.Kuraoka, M.Kawabuchi.: "Three dimensional structure of c-Kit-positive cellular networks in the guinea pig small intestine and colon" Cell and Tissue Research. (in oress).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Hirata, Z.Chogjian, K.Nakamura, M.Kawabuchi.: "Postnatal development of Schwann cells at neuromuscular junctions, with special reference to synapse elimination." J.Neurocytol.26. 799-809 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.T.M.S.Islam, K.Nakamura, M.Kawabuchi.: "Expression of NCAM-H and S100 protein in the rostral migratory stream of the adult guinea pig forebrain" Acta Histoch.Cytoch.30. 315-318 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Isayama, H.Nakamura, H.Kanemaru, M.Kawabuchi, N.Tashiro: "Distribution and co-localization of nitric oxide synthase and arginosuccinate synthetase in the cat hypothalamus." Arch.Histol.Cytol.60. 477-492 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.T.M.S.Islam, A.Kuraoka, T.Seki, A.Kuraoka, K.Hirata, M.Kawabuchi: "Neuronal PSA-N-CAM immunopositive clusters in the adult guinea pig forebrain" Abstr.Soc.Neurosci.27th Meeting. 23. 871 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Kawabuchi, Z.Chongjian, , K.Hirata: "The effect of aging on reinnervation to the motor endplate." Acta Anat.Jap.72. 360 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Kawabuchi, K.Nakamura, K.Hirata, A.T.M.S.Islam, T.Watanabe: "Amorphplogical study of thymus stromal cells (TEL-2cell) which play arole in the elimination of double positive immature thymocytes by phagocytosis." Anat.Rec. 244. 271-283 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.T.M.S.Islam, K.Nakamura, T.Seki, K.Hirata, M.Kawabuchi: "Expression ofNOS,NCAM-H and S100 protein in the granule cell generation pathway of the adult guinea pig olfactory bulb" Abstr.Soc.Neuro-sci.26th Meeting. 22. 980 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] M.Kawabuchi Z.Chongjian, K.Nakammura, K.Hirata: "Morphological features of collateral innervation and polyneuronal innervation in the skeletal muscle of presenile rats." Ann.Anat.177. 251-2656 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Masaru Kawabuchi: "The effect of aging on the morphological nerve changes during nerve regeneration" Restorative Neruorolgy & Neruoscience. (accepted).

    • Related Report
      1997 Annual Research Report
  • [Publications] A.T.M.S.Islam: "Expression of NOS,P PSA-N-CAM and S100 protein in the granule cell maturation pathway of the adult guinea pig forebrain" Developmental Braine Research. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] A.T.M.S.Islam: "Olfactory granule cell dispersion in the adult guinea pig forebrain" Brain Research Protocols. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Hiroki Tohma: "Three dimensional structure of c-Kit-positive cellular networks in the guinea pig small intestine and colon" Cell and Tissue Research. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Kazuho Hirata: "Postnatal development of Schwann cells at neruomuscular junctions,with special rererence to synapse elimination" Journal of Neruocytology. 26. 799-809 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] A.T.M.S.Islam: "Expression of N-CAM-H ans S100 protein in the rostral migratory stream of the adult guinea pig" Acta Histochemica et Cytochemica. 30. 315-318 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masaru Kawabuchi: "Neuronal PSA-N-CAM immunopositive clusters in the adult guinea pig forebrain" Abstracts Society for Neuroscience 27th Annulal Meeting. 23. 871 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hiroyuki Isayama: "Distribution and co-localization of nitric oxide synthase and arginosuccinate synhthetase in the cat hypothalamus" Archieves of Histology and Cytology. 60. 477-492 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masaru Kawabuchi: "The effect of aging on reinnervation to the motor endplate" Acta Anatomica Japonica. 72. 360 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masaru Kawabuchi: "A morphological study of thymus stromal cells(TEL-2 cell)which play a role in the elimination of doulble positive immature thymocytes by phagocytosis" Anatomical Record. 244. 271-283 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masaru Kawabuchi: "Expression of NOS,NCAM-H,and S100 protein in the granule cell generation of the adult guinea pig olfactory bulb" Abstracts Society for Neuroscience 26th Annulal Meeting. 22. 980 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masaru Kawabuchi: "Morphological features of collateral innervation and polyneuronal innervationin the skeletal muscle of presenile rats" Annals of Anatomy. 177. 251-265 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] Masaru Kawabuchi: "Variations of collateral branching in the motor nerve fibers in the skeletal muscle fibers of adult rats" Acta Histochemica. 94. 173-182 (1992)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masaru Kawabuchi: "骨格筋における神経再支配について" Fukuoka Acta Medica. 84. 111-115 (1993)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masaru Kawabuchi: "Morphological features of collateral innervation and supernumerary innervation in the skeletal muscles of presenile rats" Annals of Anatomy. 177. 251-265 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masaru Kawabuchi: "運動終板における多重神経支配について" Acta Anatomica Nipponica. 71. 495- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masaru Kawabuchi: "A morphological study of the thymus stromal cells (TEL-2 cell) which play a role in eliminaiton of immature thymocyttes by phagocytosis" Anatomical Record. 244. 271-283 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Masaru Kawabuchi: "Expression of NOS,NCAM-H,and S100 protein in the granule cell generation pathway of the adult guinear pig olfactory bulb" Society for Neuroscience Abstracts. 22. 980- (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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