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The destiny of the neural crest cells where the homeobox gene was ectopically expressed.

Research Project

Project/Area Number 08670036
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionTokai University

Principal Investigator

TANIGUCHI Yasushi  Tokai University School of Medecine Department of Molecular LIfe Science Lecturer, 医学部, 講師 (30207188)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1997: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordshomeobox / HOXD3 / cell adhesion / integrin / cadherin / Wnt-1 / neural crest / transgenic mouse / トランスジェニックマウス / インテグリンβ3 / R-カドヘリン / トアンスジェニックマウス / 細胞接着因子
Research Abstract

I isolated the human homeobox gene HOXD3 on chromosome 2 from a human genomic library and determined its nucleotide sequence. To study the role of HOXD3 gene in erythropoiesis, expression vectors containing the HOXD3 gene in the sense or antisense orientation were introduced into HEL cells. The sense transfectants overexpressing the HOXD3 gene attached more efficiently to fibronectin and collagen than did the antisense transfectants and parental HEL cells. Northern blot analysis showed that integrin beta3 mRNA levels were significantly increased in the HEL cells overexpressing the HOXD3 gene, whereas the integrin beta1 and alphaIIb mRNA levels did not show a distinct correlation with HOXD3 mRNA levels. Fluorescense-activated cell sorting analysis showed that the sense transfectants overexpressing the HOXD3 gene expressed increased levels of integrin alphaIIbbeta3 (GP IIbIIIa) complex as compared with the parental HEL cells and antisense transfectants. In addition, reverse transcriptase-mediated polymerase chain reaction (RT-PCR) analysis revealed that R-cadherin mRNA Ievels were significantly increased in the antisense transfectants expressing very low levels of the HOXD3 mRNA as compared to the sense transfectants and parental HEL cells. These results implicate the homeobox gene HOXD3 in the regulation of cell adhesion process. To verify the regulation by the HOXD3 also in developing mouse embryos, I made transgenic mice where the human HOXD3 gene was specifically expressed in the neural crest cells and analyzed the differentiation and adhessiveness of the cells.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 谷口 泰史: "ホメオボックス遺伝子HOXD3によるヒト白血病細胞接着分子発現の制御" 細胞. 28. 250-254 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Taniguchi, Y.: "Cloning,sequencing,and chromosomal localization of two tandemly arranged human pseudoganes for the proliferating cell nuclear antigeu (RCNA)" Manualian Genome. 7. 906-908 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Taniguchi, Y.: "Isalation and structural analysis of the mouse Nat (Ring3) gene in the MHC class II region." Nucleic Acids Symp.Ser.35. 225-226 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Taniguchi, Y.: "Nucleotide sequence of the Ring3 gene in the class II region of the mouse MHC and its abundant expression in testicular germ cells." Genomics. 印刷中. (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Taniguchi, Y.: "Regulation of the expression of cell adhesion molecules by the homeobox gene HOXD3 in human leukemia cells." The cell. 28. 250-254 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Taniguchi, Y., Katsumata, Y., Koido, S., Suemizu, H., Yoshimura, S., Moriuchi, T., Kagotani, K., Taguchi, H., Imanishi, T., Gojobori , T., and Inoko, H.: "Cloning, sequencing, and chromosomal localization of two tandemly arranged human pseudogenes for the proliferating cell nuclear antigen (PCNA)" Mamm.Genom. 7. 906-908 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsuzaka, Y., Kagotani, K., Okumura, K., Kimura, M., Inoko H., and Taniguchi, Y.: "Isolation and structural analysis of the mouse Nat (Ring3) gene in the MHC class II region." Nucleic Acids Symp.Ser.35. 225-226 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Taniguchi, Y., Matsuzaka, Y., Fujimoto, H.Miyado, K., Kohda A., Okumura, K., Kimura, M., and Inoko, H.: "Nucleotide sequence of the Ring3 gene in the class II region of the mouse MHC and its abundant expression in testicular germ cells." Genomics. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Taniguchi,Y.: "Nucleotide sequence of the Ring3 gene in the class II region of the mouse MHC and its abundant expression in testicular germ cells." Genomics. (印刷中). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] 谷口泰史: "ホメオボックス遺伝子HOXD3によるとヒト白血病細胞接着分子発現の制御" 細胞. 28・7. 250-254 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Taniguchi: "Isolation and structural analysis of the mouse Nat (Ring3) gene in the MHC class II region" Nucl.Acids Symp.Ser.35. 225-226 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Y.Taniguchi: "Cloning,sequencing,and chromosomal localization of two tandenly arranged human psedogenes for the preliferating cell nuclear antigen" Mammalian Genome. 7/12. 906-908 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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