Cloning of left-right determining (inv) gene
| Project/Area Number |
08670039
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | Tokyo Women's Medical College |
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Principal Investigator |
YOKOYAMA Takahiko Tokyo Women's Medical College Department of Anatomy and Developmental Biology, Joshu, 医学部, 助手 (70191525)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | asymmetry / sirus inversus / mutant / inv / ankyrin / gene / YAC / トランスジェニックマウス |
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| Research Abstract |
Almost all internal organs of vertebrate body have distinctive left-right asymmetry. The inv mutation was found in a family of transgenic mice in which tyrosinase minigene was introduced. Homozygous inv mice show a consistent reversal of left/right polarity, in contrast to randomization of the left-right asymmetry reported in other genetic and experimentally induced situs inversus mutants. Since no other tyrosinase transgenic families of mice show any abnormalities of the left-right asymmetry, the inv mutant is considered to be caused by insertional mutation and the transgenic integration destroyed an essential gene or genes to establish handed asymmetry. We cloned a candidate gene, which is responsible for reversal of left-right asymmetry in the inv mutation.
The deduced amino acid sequence of the candidate gene encodes 1062 amino acids and has 15 consecutive repeats of Ank/swi6 motif in its N-terminal half. The gene starts expressing as early as embryonic day 7 in mice and that kidney and liver show the highest level of expression among tissues in adult mice. Ank/swi6 motif is implicated to bind proteins. We assume that the inv gene product creates asymmetric distribution of intracellular molecules by binding other proteins with its Ank/swi6 motif, then establishes cell polarity and that this cell polarity induces downstream cascade like asymmetric expression of cell signaling molecules and receptor.
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Report
(2 results)
Research Products
(9 results)
