Analysis of the intracellular signal pathway in the pancreatic b-cells by direct observation of exocytosis
| Project/Area Number |
08670046
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
TERAKAWA Susumu Hamamatsu University School of Medicine, Photon Medical Research Center, Professor, 光量子医学研究センター, 文部教官教授 (50014246)
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| Co-Investigator(Kenkyū-buntansha) |
SAKURAI Takashi Hamamatsu University School of Medicine, Photon Medical Research Center, Researc, 光量子医学研究センター, 文部教官助手 (50283362)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1996: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | beta-cell / exocytosis / insulin / super oxide / chemiluminescence / video microscopy / vitamin E / MCLA / ラジカル |
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| Research Abstract |
The purpose of the present study is to clarify the involvement of major radical species on the insulin release from the pancreatic beta-cells. To this end, we applied NO,O_<2">, and alpha-tochopherol on the islet cells. These radicals induced degranulation of the beta-cells. We also observed chemiluminescene in the pancreatic tissue in vivo perfused with MCLA containing medium. Stimulation of the tissue with glucose significantly increased the MCLA-dependent chemiluminescence especially in the vicinity of the islet. Therefore, it was concluded that elevation in the glucose concentration results in radical production in the tissue and radicals induces degranulation in the beta-cells. alpha-tocopherol also induced degranulation in the same tissue. This effect of alpha-tocopherol was blocked by some types of bovine serum albumin. The present study clarified that radicals have not only adverse effects on the beta-cell but also physiologically important role in signal pathway leading to insulin release. It should be also emphasized that the beta-cells has a special sensitivity to radicals, and some radical scavengers act as radical species in the b-cells,
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Report
(3 results)
Research Products
(21 results)
