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Nitroxidergic cerebrocortical vasomotor mechanisms.

Research Project

Project/Area Number 08670073
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionNational Cardiovascular Center Research Institute

Principal Investigator

NAKAI Masatsugu  Natl.Cardiovasc.Ctr.Res.Inst. Chief Investigator, 循環動態機能部, 室長 (90150226)

Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥800,000 (Direct Cost: ¥800,000)
KeywordsNitric oxide / Cerebral cortex / Cerebral blood flow / Muscarinic receptor / NMDA receptor / Excitatory aminoacid receptors / Periaqueductal gray / Nucleus basalis magnocellularis / 血流量 / アセチルコリン / 興奮性アミノ酸 / 皮質投射
Research Abstract

The periaqueductal gray matter (PAG) is implicated in the central processing of defensive reactions. We found that when stimulated by microinjection of NMDA, the caudal third of the lateral PAG was also capable of increasing cerebral cortical blood flow (CoBF) over widespread cortical areas, which was accompanied by a cortical activation as indicated by observations of enhanced cortical energy metabolism. A substantial proportion of the flow increase was inhibited by the topical cortical application of an inhibitor of nitric oxide (NO) synthase. We found further that the PAG-elicited CoBF increase was sensitive to scopolamine and to MK-801, and attenuated by a chemical impairment of the nucleus basalis magnocellularis (NBM), the latter of which procedure also resulted in a considerable attenuation of cortical ChAT activity. An Ach-induced CoBF increase was highly sensitive not only to scopolamine but also to MK-801. In contrast, a NMDA-induced CoBF increase was sensitive to MK-801, but … More resistant to scopolamine, Synergism between the corticovasomotor actions of Ach and NMDA was very weak.
Overall, we consider that the PAG subdivision provides excitatory inputs to the NBM, which in turn sends off axons to the cortical EAAergic neurons which possess muscarinic receptors. This cholinergic/EAAergic neuron chain may form a major serial link in the central circuitry which emanates from the PAG subdivision and finds its final target site in the cortical primary vasomotor mechanisms. The vasomotor mechanisms may possess NMDA receptors and include the cortical nitroxidergic and metabolic mechanisms.
We further attempted to measure NO contents in the cerebral arterial blood and in the superior sagittal sinus blood so as to estimate cortical production of NO.We found that despite the PAG was stimulatied strongly and thereby resulting in a great increase in CoBF, any detectable difference in NO contents was not observed between arterial blood and sinus blood. We conclude therefore that this approach is of no use for an evaluation of changes in cortical NO production upon physiological stimuli. Less

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Nakai M, Maeda M: "Vasodilatation and enhanced oxidative metabolism of thecerebral cortex provoked by the periaqueductal gray matter in anaesthetized rats." Neuroscience. 72. 1133-1140 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakai M, Maeda M: "Nitrergic cerebral vasodilatation provoked by the periaqueductal gray." Neuroreport. 7. 2571-2574 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 中井, 緒方, 玉城, 山本, 福井 松井, 前田: "脳循環調節中枢." 神経研究の進歩. 40. 327-339 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakai M, Ogata J, Fukui Y, Nakai Y, Maeda M: "Basal forebrain and cerebral cortical muscarinic receptors mediate increase in cortical blood flow provoked by periaqueductal gray matter." Neuroscience. 79. 571-579 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakai M, Maeda M.: "Nitrergic cerebral vasodilatation provoked by the periaqueductal grey." Neuroreport. 7. 15-17 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakai M, Maeda M.: "Vasodilatation and enhanced oxidative metabolism of the cerebral cortex provoked by the periaque-ductal gray matter in anaesthetized rats." Neuroscience. 72. 1133-1140 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakai M, Ogata J, Fukui Y, Nakai Y, Maeda M: "Basal forebrain and cerebral cortical muscarinic receptors mediate increase in cortical blood flow provoked by the periaqueductal gray matter." Neuroscience. 79. 571-579 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nakai M: "Basal forebrain and cerebral cortical muscarinic receptors mediate increase in cortical blood flow provoked by periaqueductal gray matter." Neuroscience. 79. 571-579 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 中井正繼 他: "脳循環調節中枢" 神経研究の進歩(脳循環と脳虚血). 40. 327-339 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nakai M: "Vasodilation and enhanced oxidative metabolism of the cerebral cortex provoked by the periaqueductal gray matter in anaesthetized rats." Neuroscience. 72. 1133-1140 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nakai M: "Nitrergic cerebral vasodilation provoked by the periaqueductal gray." Neuroreport. 7. 2571-2574 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Nakai M: "Basal forebrain and cerebral cortical muscarinic cholinergic mechanism mediate increase in cortical blood flow provoked by periaqueductal gray matter." Neuroscience. (in press). (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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