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AGING EFFECTS ON CENTRAL MECHANISM OF CIRCADIAN RHYTHM

Research Project

Project/Area Number 08670088
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental physiology (including Physical medicine and Nutritional physiology)
Research InstitutionDOKKYO UNIVERSITY SCHOOL OF MEDICINE

Principal Investigator

YAMAOKA Sadao  Dokkyo University School of Medicine Professor, 医学部, 教授 (50049813)

Co-Investigator(Kenkyū-buntansha) WATANABE Kazuto  Dokkyo University School of Medicine Instructor, 医学部, 助手 (80146167)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 1997: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsCircadian rhythm / Aging / Suprachiasmatic nucleus / Fos-like immunoreactive cell / Zitter rat / Diabetes insipidus rat / Light pulse stimulation / Re-entraining period / Fos様蛋白 / 光同調性 / 免疫組織化学
Research Abstract

Age-related changes in various circadian rhythms have been documented. However aging mechanisms in circadian system is still unknown. To clarify the role of suprachiasmatic nucleus (SCN) in correlation between aging mechanism and circadian system, we adapted the analyzes of circadian locomotor activity and the quantitative immunohistochemistry. 1) .Male Sprague Dawley rats (SD) and diabetes insipidus (DI) rats were divided into three groups ; 2-5,12-16 and 18-30 months age. Zitter rat (zi), which shows central spongy degeneration, divided two groups (1-3 and 7-10 months age). The circadian locomotor activity (LMA) was measured by infra-red area sensor. The free-running period under constant dark shortened with age in all strains. Two hours light pulse caused the phase shift depending on the applied circadian time in young SD rat, but not in old SD,DI and 7-10 months zi rat. In old SD rats, the re-entraining days to reverse lighting schedule postponed with age. These aging effects of 7-10 months zi rats were equivalent with over 24 months SD rats. 2).The Fos-like immunoreactive (Fos-IR) cells was diffusely located in SCN.Greater amount of Fos-IR cells were seen in light phase than dark phase under light-dark condition. This change was disrupted in old rat. Light induced Fos-IR cells were confined in the ventrolateral part of SCN during constant dark, and were expressed more in subjective night than day. This change did not show any difference with the age. These results suggest that a part of aging mechanism in circadian rhythm due to the disruption of transduction from circadian oscillator, and the signal transduction from retina is not affected by aging.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (38 results)

All Other

All Publications (38 results)

  • [Publications] Y.Saitoh, T, Kitajima, S.Yamaoka and S.Ueda: "Degenerative changes in the serotonergic fibers in the spinal cord of zitter mutant rat." Acta Histochem.et Cytochem.29(3). 265-268 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Koibuchi, S.Matsuzaki, K.Ichimura, H.Ohtake and S.Yamaoka: "Ontogenic changes in the expression of cytochrome c oxidase subuni I gene in the cerebeller cortex of the perinatal hypothyroid rat." Endocrinology. 137(11). 5096-5108 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.H.Meijer, K.Watanabe, L.Detari and J.Schaap.: "Circadian rhythm in light response in suprachiasmatic nucleus neurons of freely moving rats." Brain Research. 741. 352-355 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Yamaoka, N.Koibuchi, K.Watanabe and M.Sakai: "Are zitter rats an animal model for studying the aging of circadian rhythm?" Jpn.J.Physiology. 46(suppl). S173 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Watanabe and S.Yamaoka.: "The free-running period in cell cultures of rat suprachiasmatic nucleus." Jpn.J.Physiology. 46(suppl). S173 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Yamazaki, K.Watanabe, Z.Qu, S.Yamaoka, N.Ishida.: "Efficient gene expression in mammalian clock pacemaker cell in vitro by an adenovirus vector." Neurosci.Lett.221. 193-196 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Watanabe and S.Yamaoka: "Cell cultures of rat suprachiasmatic nucleus:Circadian oscillation of vasopressin release." Biological Rhythm research,. 28・3. 299-305 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.H.Meijer, J.Schaap, K.Watanabe and H.Albus.: "Multiunit activity recording in the suprachiasmatic nuclei:in vivo versus in vitro models." Brain Research. 753:. 322-327, (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Watanabe and S.Yamaoka: "Changes in phase and period length in circadian oscillation of vasopressin release in cell cultures of rat suprachiasmatic nucleus." Jpn.J.Physiol.47・suppl.S32 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Yamaoka, K.Watanabe, M.Sakai: "Circadian rhythmicity of zitter rats,diabetes insipidus rats, and hypothyroid rats." Jpn.J.Physiol.47・suppl.S195 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.H.Meijer, k.watanabe, L.Detari, M.J.de Vries, H.Albus, J.A.Treep, J.Schaap and W.J.Rietveld.: "Light entrainment of the mammalian biological clock.In"Hypothalamic Integration of Circadian Rhythms"Progress in Brain Research,Vol.111" Eds.R.M.Buijs,A.Kalsbeek,H.J.Romijn,C.M.A.Pennartz and M.Mirmiran,ELSEVIER,AMSTERDAM, 175-190, (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Watanaba and S.Yamaoka: "Cultures of dissociated rat suprachiasmatic nucleus cells.In"Circadian Organization and oscieeatory Couplin"" Eds.K.Homma and S.Homma,Hokkaido Univ.Press,Sapporo, 137-146 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Y.Saitoh, T.Kitajima, S.Yamaoka, and S.Ueda: "Degenerative changes in the serotonergic fibers in the spinal cord of zitter mutant rat" Acta Histochem.et Cytochem.29. 265-268 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.koibuchi, S.Matsuzaki, K,Ichimura, H.Ohtake and S.Yamaoka: "Ontogenic changes in the expression of cytochrome c oxidase subuni I gene in the cerebeller cortex of the perinatal hypothyroid rat" Endocrinology. 137(11). 5096-5108 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Tanaka, N.Koibuchi, H.Ohtake, T.Kawatsu, N.Tadokoro, T.Kumasaka, N.Inaba and S.Yamaoka: "Regional comparison of prolactin gene expression in the human decidualized endometrium in early and term pregnancy." Eur.J.Endocrinol.135. 177-183 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.H.Meijer, K.Watanabe, L.Detari and J.Schaap: "Circadian rhythm in light response in suprachiasmatic nucleus neurons of freely moving rats." Brain Research. 741. 352-355 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Yamaoka, N.Koibuchi, K.Watanabe and M.Sakai: "Are zitter rats an animal model for studying the aging of circadian rhythm?" Jpn.J.Physiology. 46 (suppl). S173 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Watanabe and S.Yamaoka: "The free-running period in cell cultures of rat suprachiasmatic nucleus." Jpn.J.Physiology. 46 (suppl). S173 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Watanabe and S.Yamaoka: "Effect of glutamate on circadian oscillation of vasopressin release in cell culture of rat suprachiasmatic nucleus." Neurosci.Res.20. S233 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Watanabe and S.Yamaoka: "Circadian oscilation of vasopressin release in cell cultures of rat suprachiasmatic nucleus." Chronobil. Int.13 (supple). 133 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.yamazaki, K.Watanabe, Z.Qu, S.Yamaoka and N.Ishida.: "Efficient gene expression in mmammalian clock pacemaker cell in vitro by an adenovirus" Neurosci.Lett.221. 193-196 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.H.Meijer, J.Schaap, K.Watanabe and H.Albus.: "Multiunit activity recording in the suprachiasmatic nuclei : in vivo versus in vitro models." Brain Research. 753. 322-327 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Watanabe and S.Yamaoka: "Cell cultures of rat suprachiasmatic nucleus : circadian oscillation of vasopressin release." Biolgical Rhythm Res.28(3). 299-305 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Watanabe and S.Yamaoka: "Changes in phase and period length in circadian oscillation of vasopressin release in cell culture of rat suprachiasmatic nucleus." Jpn.J.Physiol.47 (suppl.). S32 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] S.Yamaoka, K.Watanabe and M.Sakai: "Circadian rhythmicity of zitter rats, diabetes insipidus rats, and hypothyroid rats." Jpn.J.Physiol.47 (suppl.). S195 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.H.Meijer, K.Watanabe, L.Detari, M.J.de Vries, H.Albus, J.A.Treep, J.Schaap and W.J.Rietveld: In "Hypothalamic Integration of Circadian Rhythms" Eds.R.M.Buijs, A.Kalsbeek, H.J.Romijn, C.M.A.Dennartz and M.Mirmiran, Progress in Brain Research, Vol.111. Light entrainment of the mammalian biological clock., 175-190 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Watanabe and S.Yamaoka: Cultures of dissociated rat suprachiasmatic nucleus cells. In "Circadian Organization and oscillatory Cuopling" eds.K.Homma and S.Homma. Hokkaido Univ.Press, Sapporo, 137-146 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Yamazaki, K.Watanabe, Z.Qu, S.Yamaoka, N.Ishida,: "Efficient gene expression in mammalian clock pacemaker cell in vitro by an adenovirus vector." Neurosci.Lett.221. 193-196 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Watanabe and S.Yamaoka: "Cell cultures of rat suprachiasmatic nucleus : Circadian oscillation of vasopressin release." Biological Rhythm Research,. 28・3. 299-305 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] K.Watanabe and S.Yamaoka: "Changes in phase and period length in circadian oscillation of vasopressin release in cell cultures of rat suprachiasmatic nucleus." Jpn.J.Physiol.47・suppl.S32 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] S.Yamaoka, K.Watanabe, M.Sakai: "Circadian rhythmicity of zitter rats,diabetes insipidus rats,and hypothyroid rats." Jpn.J.Physiol.47・suppl.S195 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yamaoka S,Koibuchi N,Watanabe K and Sakai M: "Are Zitter rats an animal model for studying the aging of the circadian rhythm ?" The Japanese Journal of Physiology. 46(Suppl). S173- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Watanabe K and Yamaoka S: "The free-running period in cell cultures of rat suprachiasmatic nucleus" The Japanese Journal of Physiology. 46(Suppl). S173- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kazuto Watanabe,Sadao Yamaoka: "Effect of glutamate on circadian oscillation of vasopressin release in cell cultures of rat suprachiasmatic nucleus." Neuroscience research. 20(Suppl). S233- (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Noriyuki Koibuchi,Shigeru Matsuzaki,Kaoru Ichimura,Hideki Ohtake and Sadao Yamaoka: "Ontogenic changes in the expression of cytochrome c oxidase subunit I gene in the cerebellar cortex of the perinatal hypothyroid rat." Endocrinology. 137(11). 5096-5108 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yuuki Saitoh,Toshimitsu Kitajima,Sadao Yamaoka and Shuichi Ueda: "Degenerative changes in the serotonergic filbers in the spinal cord of Zitter mutant rat." Acta Histochemica et Cytochemica. 29(3). 265-268 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Norihiko Yamazaki,Kazuto Watanabe,Zhiqiang Qu,Sadao Yamaoka and Norio Ishida: "Efficient gene expression in mammalian clock pacemaker cell in vitro by an adenovirus vector." Neuroscience Letters. 221. 193-196 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Kazuto Watanabe and Sadao Yamaoka: ""Circadian Organization and Oscillatory Coupling" Eds.K.Honma and S.Honma" Hokkaido University Press,Sappro, 10 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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