| Project/Area Number |
08670195
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Niigata University |
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Principal Investigator |
IWAFUCHI Mitsuya Niigata University, College of Biomedical Technology, Department of Medical Technology, Professor (Pathology), 医療技術短期大学部, 教授 (70143766)
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| Co-Investigator(Kenkyū-buntansha) |
SIGENO Naoya Niigata University, College of Biomedical Technology, Department of Medical Tech, 医療技術短期大学部, 教授 (60018330)
WATANABE Hidenobu Niigata University, School of Medicine, Department of Pathology, Professor (Path, 医学部, 教授 (70037381)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Carcinoid / Endocrine cell carcinoma / Small cell carcinoma / Paraneuroma / Gut hormone / Cell culture |
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| Research Abstract |
During the period of past two years of this project, about 400 gastrointestinal endocrine cell tumors (GECT) of Japanese and six cell lines of endocrine cell carcinoma were analyzed.
1. CLASSIFICATION : GECT were divided into two main groups : carcinoid tumor (CD), low-grade malignancy and endocrine cell carcinoma (ECC), high-grade malignacy.Histological differential diagnosis and the new classification according to ocurring sites of GECT were established.
2. HISTOGENESIS : CD was suspected to be mainly derived from immature endocrine cell. ECC was hypothesized to derive from neoplastic endocrine cell in mucosal tubular adenocarcinoma/adenoma, totipotential stem cell, CD,or immature endocrine cell. The first pathway was suspected to be most frequent, followed by the second. Difference in p53 protein overexpression between CD and ECC confirmed the difference in their histogenetic pathway.
3. PROGRESSION AND METASTASIS : Cell proliferation activity of GECT was confirmed to correlate well with tumor progression and metastasis. For clinical hormone analysis to assess tumor recurrence and metastasis, GECT should be subdivided into three groups ; argentaffin (serotonin-producing) CD,argyrophi1 (peptide-producing) CD,and ECC (frequently serotonin-producing).
4. CELL LINES : Four cell lines established from human esophageal, gastric and rectal ECC well preserved hormone (serotonin, peptide YY,calcitonin)-production. One esophageal cell line was effected by Vincristin and Mitomycin.
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