| Project/Area Number |
08670196
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Kanazawa University |
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Principal Investigator |
MIZUKAMI Yuji Kanazawa University, School of Medicine, Professor, 医学部, 教授 (60110540)
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| Co-Investigator(Kenkyū-buntansha) |
MICHIGISHI Takatoshi Kanazawa University, School of Medicine, Associate Professor, 医学部, 助教授 (60020012)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | TSH-receptor / thyroid / immunohistochemistry / in situ hybridization / northern blot hybridization / nothern blot hybridization / thyroglobulin / peroxidase |
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| Research Abstract |
In the present study, we investigated TSH-receptor expression in hyman thyroids using immunohistochemistry, in situ hybridization and northern blot. The relationship between the TSH-receptor expression and the thyroid function were examined.
A.Materials and Methoeds
The surgically resected thyroid tissues of various thyroid diseases were used in this study. The thyroid specimens were immediately frozed at -80C and stored. The TSH-receptor cDNA vector and the anti-human mouse monoclomnal antibody against TSH-receptor protein were used. These were kindly supplied by Dr.E.Milgrom (paris South University, France).
B.Results
The obtained results were as follows :
1. The immunohistochemical, in situ hybridization and northern blot study showed the same results in the expression of TSH-receptor in human thyroids of each case. examined.
2. In Graves' thyroids, the elevated TSH-receotor expression was observed in both of protein level and mRNA level. This finding supported the theory that the up-regulation mechanism of TSH-receptor is one of the major causes of Graves' hyperthyroidism.
3. In the thyroid adenomas and the carcinomas which are functionally inactive, the elevated TSH-receptor expression was observed in the protein level and mRNA level.
4. In the undifferentiated thyroid carcinomas, The TSH-receptor expression was not detected in the protein level and mRNA level.
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