| Project/Area Number |
08670199
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Yamanashi Medical University |
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Principal Investigator |
KAWAOI Akira Yamanashi Medical University, Dept.of Pathology, Professor, 医学部, 教授 (30059224)
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| Co-Investigator(Kenkyū-buntansha) |
YOKOYAMA Akira Yamanashi Medical University, Dept.of Pathology, Research Associate, 医学部, 助手 (30273056)
HEMMI Akihiro Yamanashi Medical University, Dept.of Pathology, Research Associate, 医学部, 助手 (40173562)
KATOH Ryohei Yamanashi Medical University, Dept.of Pathology, Associate Professor, 医学部, 助教授 (30152755)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | Apoptosis / Thyroid / Bone marrow derived cell / 骨髄細細 / 骨髄細胞 / IL-3 |
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| Research Abstract |
In the present study TSH dependnt apoptosis was successfully induced to FRTL-5 cells by replacing conventional monolayr culture with suspension culture.
Since cell to cell or cell to matrix adhesion was considered to be significantly weakenend in suspension culture, the adhesion molecules such as E-cadherin, integrins and their ligands such as laminin, type 4 collagen, fibronectin which are expressed in FRTL-5 cells were immunocytochemically examined, and their expression in the cells was suggested to be up-regulated by TSH in suspension culture.
The possibility was deduced from above observations that induction of TSH dependent apoptosis in FRTL-5 cells was initiated by the decreased adhesiveness of cells caused by down regulated expression of adhesion molecules in these cells.
The questions remained to be answered are as follows : 1. What is the role of TSH in the expression of adhesion molecules in monolayr cultured cells which are resistant against apoptosis? 2. Are there, if any, regulatory factors which make FRTL-5 cells resistant vs apoptosis by keeping cell adhesion stable in the condition of monolayr culture without TSH? 3. How TSH exerts its effects on the cell adhesion in FRTL-5 cells, and why the deprivation of TSH results in the loosening of cell adhesion and finally in apotosis?
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