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Chromosomal Translocations in Human Soft Tissue Sarcomas : Fluorescence in Situ Hydridization as a Diagnostic Aid

Research Project

Project/Area Number 08670203
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionTottori University

Principal Investigator

YOSHIDA Haruhiko  Tottori University College of Medical Care Technology, Professor, 医療技術短期大学部, 教授 (40037429)

Co-Investigator(Kenkyū-buntansha) NAKAMOTO Sachiko  Tottori University College of Medical Care Technology, Assistant Professor, 医療技術短期大学, 助手 (20116338)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥1,000,000 (Direct Cost: ¥1,000,000)
Keywordssoft tissue sarcoma / paraffin-block / chromosome / translocation / fluorescence in situ hybridization (FISH) / 染色体異常 / マルチカラーFISH法
Research Abstract

To evaluate fluorescence in stu hybridization (FISH) findings of soft tissue sarcomas and clarify the relation between their hisological tumor types and chromosomal rearrangements, we examined three kinds of translocations in the interphase nuclei isolated from paraffin-embedded tissues of 47 soft tissue sarcomas. Translocations were evaluated using chromosome-specific DNA probes of centromeric and whole painting probes.
Of 47 soft tissue sacomas, 42 (89.4%) revealed timor-specific translocations by etrospective chromosomal analysis. Translocation t(X ; 1 8) was detected in 25/28 synovi al sarcomas ; translocation t(11 ; 22) in 5/6 Ewing's sarcomas and primitive neuroectodermal tumors (PNET) ; and translocation t(12 ; 16) was found in 12/13 liposarcomas (LPS), including 10 myxoid and 2 round cell types as don aichrom osoma I aberrations specific for both subtypes.
Based on these findings, Ewing's sarcoma is related closely with PNET originating in neuroectodermal tissue. Other cytogenetic finding of translocation t(12 ; 16) indicates the round cell liposarcoma shares chromosomal change with myxoid LPS, and further suggests that both tumors may possess common prcursor cells.
Karyotypic analysis was performed on a case of chondromyxoid fibroma arising in 42-year-old, female and revealed a complex pattern of chromosomal abberation including t(1 ; 12).

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (28 results)

All Other

All Publications (28 results)

  • [Publications] Nagao K, Ito H and Yoshida H: "Cromosomal translocation t(X ; 18)in human synovial sarcoma analyzed by fluorescence in situ hybridization using paraffin-embeddedtissues." Am J Pathol. 148. 601-609 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Osaki M, Adachi H, Gomyo Y, Yoshida H and Ito H: "Detection of mycobacterial DNA in formalin-fixed, paraffin-embeddedtissue specimens by duplex polymerase chain reaction : Application to histopathologic diagnosis" Mod Pathol. 10. 78-83 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nagao K, Ito H, Yoshida H, Minamizaki T, Furuse K, Yoshikawa T, Ushigome S: "Chromosomal rearrangement t(11 ; 22)in extraskeletal Ewing's sarcoma and primitive neuroectodermal tumour analysed by fluorescence in situ hybridization using paraffin-embedded tissue" J Pathol. 181. 62-66 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Osaki M, Shimabayashi A, Iwao Y, Asakura Y, Adachi H, Ito H and Yoshida H reactions.: "Search for mycobacterial DNA in paraffin-embedded tissues from bone and soft-tissue tuberculosis by duplex polymerase chain Ahelpful methodto histopathological diagnosis." Bull MCT. 261. 93-98 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshida H, Nagao K, Ito H, Tamamoto K and Ushigome S: "Chromosomal translocations in human soft tissue sarcomas : Fluorescence in situ hybridization as a diagnostic aid" Pathol Int. 47. 222-229 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 吉田春彦、長尾勝人、井藤久雄: "滑膜肉腫の染色体転座のFISH法による解析。特集「軟部肉腫の組織形態と生物学的態度 : 分子生物学的アプローチによる新展開」" 病理と臨床. 16. 156-160 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nagao K,Ito H and Yoshida H: "Chromosomal Translocation t (X ; 18) in Human Synovial Sarcoma analyzed by Fluorescence in Situ Hybridization using Paraffin-Embedded Tissues." Am J Pathol. 148. 601-609 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nagao K,Ito H and Yoshida H: "Detection of Chromosomal Translocation in Soft-Tissue Sarcomas : Application of Fluorescence in Situ Hybridization to Diagnosis (in Japanese)" Pathol Clin Med. 16. 299-301 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Osaki M,Adachi H,Gomyo Y,Yoshida H and Ito H: "Detection of Mycobacterial DNA in Formalin-Fixed, Paraffin-Embedded Tissue Specimens by Duplex Polymerase Chain Reaction : Application to Histopathologic Diagnosis" Mod Pathol. 10 (1). 78-83 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nagao K,Ito H,Yoshida H,Minamizaki T,Furuse K,Yoshikawa T and Ushigome S: "Extraskeletal Ewing's Sarcoma and Chromosomal Rearrangement t (11 ; 22) in Primitive Neuroectodermal Tumour Analysed by Fluorescence in Situ Hybridization Using Paraffin-Embedded Tissue" J Pathol. 181. 62-66 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshida H,Nagao K,Ito H,Yamamoto K and Ushigome S: "Chromosomal Translocations in Human Soft Tissue Sarcomas : Fluorescence in Situ Hybridization as a Diagnostic Aid" Pathol Int. 47 (4). 222-229 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshida H,Nagao H and Ito H: "Detection of Chromosomal Abnormalities in Soft-Tissue Sarcomas by Fluorescence in Situ Hybridization (in Japanese)" Orthopedic Surg Tech. 40 (13). 1569-1574 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Yoshida H,Nagao K,Endo H,Ito H: "Search for Chromosomal Translocation by Fluorescence in Situ Hybridization Method (in Japanese)" Pathol Clin Med. 16 (2). 156-160 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nagao K,Ito H and Yoshida H: "Chromosomal translocation t (X ; 18) in human synovial sarcoma analyzed by fluorescence in situ hybridization using paraffin-embeddedtissues." Am J Pathol. 148. 601-609 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] Osaki M,Adachi H,Gomyo Y,Yoshida H and Ito H: "Detection of mycobacterial DNA in formalin-fixed,paraffin-embeddedtissue specimens by duplex polymerase chain reaction : Application to histopathologic diagnosis." Mod Pathol. 10. 78-83 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Nagao K,Ito H,Yoshida H,Minamizaki T,Furuse K,Yoshikawa T,Ushigome S: "Chromosomal rearrang ement t (11 ; 22) in extraskeletal Ewing's sarcoma and primitive neuroectodermal tumour analysed by fluorescence in situ hybridization using paraffin-embedded tissue" J Pathol. 181. 62-66 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Osaki M,Shimabayashi A,Iwao Y,Asakura Y,Adachi H,Ito H and Yoshida H reactions.: "Search for mycobacterial DNA in paraffin-embedded tissues from bone and soft-tissue tuberculosis by duplex polymerase chain A helpful method to histopathological diagnosis." Bull MCT. 261. 93-98 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Yoshida H,Nagao K,Ito H,Yamamoto K and Ushigome S: "Chromosomal translocations in human soft tissue sarcomas : Fluorescence in situ hybridization as a diagnostic aid" Pathol Int. 47. 222-229 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 吉田春彦、長尾勝人、井藤久雄: "滑膜肉腫の染色体転座のFISH法による解析。特集「軟部肉腫の組織形態と生物学的態度:分子生物学的アプローチによる新展開」" 病理と臨床. 16. 156-160 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Osaki M, Adachi H, Gomyo Y, Yoshida H and Ito H: "Detection of Mycobacterial DNA in Formalin-Fixed,Paraffin-Embedded Tissue Specimensa by Duplex Polymerase Chain Reaction : Application to Histopathologic Diagnosis" Mod Pathol. 78. 78-83 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nagao K, Ito H, Yoshida H, and et al: "Chromosomal Rearrangement t(11;12),in Extraskeletal Ewing's Sarcoma and Primitive Neuroectodermal Tumour Analysed by Fluorescence in Situ llybridization Using Paraffin Embedded Tissu" J Pathol. 181. 62-66 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoshida H, Nagao K, Ito H, Yamamoto K and Ushigome S: "Chromosomal Translocations in Human Soft Tissue Sarcomas : Fluorescence in Situ Hybridization as a Diagnostic Aid" Pathol Int. 47(4). 222-229 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Osaki M, Shimabayashi A, Iwao Y, Asakura Y, Adachi H, Ito H and Yoshida H: "Search for mycobacterial DNA in paraffin-embedded tissues from bone and soft-tissue tuberculosis by duplex polymerase chain reactions.A belpful method to histopathological diagnosis" Bull MCT. 26. 93-98 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 吉田春彦、長尾勝人、井藤久雄: "FISH法による軟部悪性腫瘍の染色体転座の検出" 整形・災害外科. 40(13). 1569-1574 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Nagao K,Ito H and Yoshida H: "Chromosomal Translocation t(X;18)in Human Synovial Sarcoma analyzed by Fluorescence in situ Hybridization using Paraffin-Embedded Tissues" Am J Pathol. 148. 601-609 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 長尾勝人、井藤久雄、吉田春彦: "病理診断と分子生物学・遺伝学:軟部悪性腫瘍における染色体転座の検出" 病理と臨床(臨時増刊). 16. 299-301 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 吉田春彦、長尾勝人、井藤久雄: "滑膜肉腫の染色体異常と分子生物学" 検査と技術. 24(10). 867-868 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Osaki M.Adachi H,Gomyo Y,Yoshida H and Ito H: "Detection of Mycobacterial DNA in Formalin-Fixed,Paraffin-Embedded Tissue Specimensa by Duplex Polymerase Chain Reaction:Applicaiton to Histopathologic Diagnosis" Mod Pathol. 10(1). 78-83 (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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