Pathological significance of apoptosis in arteriosclerosis.

• Project/Area Number: 08670209
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Human pathology
• Research Institution: Nagoya City University
• Principal Investigator: TADA Toyohiro Nagoya City University, Medical School Associate Professor, 医学部, 助教授 (20106230)
• Co-Investigator(Kenkyū-buntansha): KUNIMATSU Mitoshi Nagoya City University, Medical School Assistant Professor, 医学部, 講師 (70145746)
• Project Period (FY): 1996 – 1997
• Project Status: Completed (Fiscal Year 1997)
• Budget Amount: ¥2,200,000 (Direct Cost: ¥2,200,000); Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000); Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
• Keywords: Atherosclerosis / Apoptosis / Artery / Cell death / Smooth muscle cell / Macrophage / 血管 / 免疫組織化学

Research Abstract

Arteriosclerotic lesions show decrease in cells including smooth muscle cells and macrophages. The ame of this project is to investigate the cause and mechanism of the decrease in the cells mentioned above.
Tissue fragments obtained by atherectomy were staied with the method of so-called TUNEL to detect the cell death, that is, apoptosis. Many cells in the atherosclerotic intima showed positive stain. These apoptotic cells were composed predominantly of smooth muscle cells, and partly of macrophages.
Immunohistochemical studies were also done. Many of the smooth muscle cells in the intima of the coronary artery were positively stained with anti-Fas antigen antibody. These results suggest that the apoptosis in the atherosclerotic arteries might be induced by the mechanism of apoptosis causeed by Fas ligand which might be expressed on the T-cells in the atherosclerotic lesion.

Research Products

• [Publications] Tateyama H, Tada T 他4名: "Effects of prefixation and fixation times on apoptosis detection by in situ end-labeling of fragmented DNA." Arh.Pathol.Lab.Med.122・3. 252-255 (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tada T, Okada H 他5名: "Membrane attack complex and 20 kDa homologous restriction factor(CD59)in myocardial infarction" Virchow Arch.430. 327-332 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kasugai, K., Tada, T., 他6名: "Evidence for participation of gliostatin/platelet-derived endothelial cell growth factor in gastric ulcer healing." Life Science. 61・19. 1899-1906 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tamai, N., Tada, T.他2名: "Alteration of the endothelium by ascorbic acid deficiency in ODS rat." Nagoya Med.J.41. 141-154 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tateyama H, Tada T 他3名: "Apoptosis,bcl-2 prtotein,and fas antigen in thymic epithelial tumors." Mod.Pathol.10・12. 983-991 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sano, H., Tada, T.他8名: "Isolation of a rat histidase cDNA sequence and expression in Escherichia coli.Evidence of extrahepatic/epidermal distribution." Eur.J.Biochem.250. 212-221 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Sasaki M, Kunimatsu M 他7名: "Medical aspects of proteases and protease inhibitors." IOS Press, 14 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Tada T,Okada H,Okada N,Tateyama H,Suzuki H,Takahashi Y,Eimoto T.: "Membrane attack complex and 20 kDa homologous restriction factor (CD59) in myocardial infarction." Virchow Arch.430. 327-332 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Karasawa, K., Tada, T., Kanai, H., Takagi, T., Yamada, K.: "Nucleolar organizing resions in primary intracranial malignant lymphomas." J.Neuro-Oncol.35. 1-6 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Tateyama, H., Tada, T., Hattori, H., Murase, T., Li, W-X., Eimoto, T.: "Effects of prefixation and fixation times on apoptosis detection by in situ end-labeling of fragmented DNA." Arh.Pathol.Lab.Med.122. 252-255 (1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kasugai, K., Takashi, J., Kataoka, H., Sasaki.M., Tada, T., Asai, K., Kato, T., Itoh, M.: "Evidence for participation of gliostatin/platelet-derived endothelial cell growth factor in gastric ulcer healing." Life Science. 61. 1899-1906 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Tamai, N., Fujinami, T., Tada, T., Eimoto, T.: "Alteration of the endothelium by ascorbic acid deficiency in ODS rat." Nagoya Med.J.41. 141-154 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Tateyama, H., Eimoto, T., Tada, T., Inagaki, H., Hattori, H., Takino, H.: "Apoptosis, bcl-2 prtotein, and fas antigen in thymic epithelial tumors." Mod.Pathol.10. 983-991 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sano, H., Tada, T., Moriyama, A., Ogawa, H., Asai, K., Kawai, Y., Hodgson, M.E., Kato, T., Wada, Y., Suchi, M.: "Isolation of a rat histidase cDNA sequence and expression in Escherichia coli.Evidence of extrahepatic/epidermal distribution." Eur.J.Biochem.250. 212-221 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Tada, T., Tateyama, H., Li, W-S., Murase, T., Eimoto, T.: "Apoptosis" The lipid. 7-9. 494-499 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Sasaki M,Kunimatsu M,Ozaki Y,Liu Z-Q,Uehara F,Onizuka K,Nishino H,Yang J-P,Okamoto T.: Selective neuronal cell death induced by activation of mu-calpain under conditions of ischemia/reperfusion.Medical aspects of proteases and protease inhibitors.Katsunuma N et al(Eds). IOS press, 43-57 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Tateyama H, Tada T, 他4名: "Effects of prefixation and fixation times on apoptosis detection by in situ end-labeling of fragmented DNA." Arh.Pathol.Lab.Med.(発表予定).
• [Publications] Tada T, Okada H, 他5名: "Membrane attack complex and 20 kDa homologous restriction factor(CD59)in myocardial infarction" Virchow Arch.430. 327-332 (1997)
• [Publications] Tateyama H, Tada T, 他3名: "Apoptosis,bcl-2 prtotein,and fas antigen in thymic epithelial tumors." Mod.Pathol.10・12. 983-991 (1997)
• [Publications] Sasaki M, Kunimatsu M, 他7名: "Medical aspects of proteases and protease inhibitors." IOS Press, 14 (1997)
• [Publications] 多田豊曠: "大動脈瘤における細胞死の組織形態学的検討" 日本病理学会会誌. 85・1. 159-159 (1996)
• [Publications] 多田豊曠: "アポトーシス" The Lipid. 7・5. 494-499 (1996)
• [Publications] Tada, T.: "Membrane attack complex of complement and 20KD homologous restriction factor (CD59) in myocardial infarction." Virchows Archiv. (発表予定).