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Analysis of a novel gene RA3, expression of which is upregulated during cell proliferation

Research Project

Project/Area Number 08670238
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionChiba University

Principal Investigator

SHIRASAWA Hiroshi  Microbiology I, Chiba University Professor, 医学部, 教授 (00216194)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
KeywordsHPV / E6 / cell proliferation
Research Abstract

Cellular genes affected by human papillomavirus type 16 (HPV16) E6 protein were cloned by a differential hybridization technique. Two thirds of the cDNA clones were those that regulated during the cell cycle, such as HMG-IY (high mobility group protein-I[Y]), prothymosin-alpha, or ornithine decarboxylase. An unknown cDNA clone, RA3 was expressed during cell proliferation, and the expression was repressed by cell contact inhibition. Furthermore, repression of the RA3 expression was relieved by the expression of HPV16 E6.
The RA3 cDNA was 1.3kb in length, and have no homology to other known genes. No known motif was also found in this new gene.
The expression of the RA3 gene was correlated with the expression of the prothymosin-alpha and JUN.The expression of the prothymosin-alpha was activated by the HPV16E6 through the E2F motif of the c-myc gene. It was revealed that heterodimer complex of JUN and ATF2 is induced by the HPV16E6, followed by activation of genes that has CRE motif.
Taken together, the RA3 gene appears to be regulated by the c-myc or JUN/ATF2 and involved in the mechanisms of the cell proliferation.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Kinoshita et al.: "Human papillomavirus type 16E6 protein up-regulates the expression of the high mobility group protein HMG-I(Y)gene in mouse 10T1/2 cells" Virus Research. 42. 119-125 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kinoshita et al.: "Transactivation of prothymosin-α and c-myc promoters by human papillomavirus type 16E6 protein." Virology. 232. 53-61 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shino et al.: "Human papillomavirus type16E6 protein transcriptionally modulates fibronectin gene expression by induction of protein complexes binding to the CAMP response element" J.Virol.71. 4310-4318 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kinoshita, T., Shirasawa, H., Shino, Y., Shimizu, K., Moriya, H.and Simizu, B: "Human papillomavirus type 16 E6 protein up-regulates the expression of the high mobility group protein HMG-I (Y) gene in mouse 10T1/2 cells." Virus Res.42. 119-125 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kinoshita, T., Shirasawa, H., Shino, Y., Moriya, H., Desbarats, L., Eilers, M.and Simizu, B.: "Transactivation of prothymosin-alpha and c-myc promoters by human papillomavirus type 16 E6 protein." Virology. 232. 53-61 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shino, Y., Shirasawa, H., Kinoshita, T.and Simizu, B.: "Human papillomavirus type 16 E6 protein transcriptionally modulates fibronectin gene expression by induction of protein complexes binding to the cAMP response element." J.Virol.71. 4310-4318 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kinoshita et al: "Human papillomavirus type 16 E6protein up-regulates the expression of the high mobility group protein HMG-I (Y) gene in mouse 10T 1/2 cells :" Virus Research. 42. 119-125 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kinoshita et al.: "Transactivation of prothymosin-α and, c-myc promoters by human papillomavirus type16 E6 protein." Virology. 232. 53-61 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shino et al: "Human papillomavirus type 16 E6 protein transcriptionally modulates fibronectin gene expression by induction of protein complexes binding to the cAMP response element." J.virol. 71. 4310-4318 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Kinoshita et al.: "Human papillomavirus type 16 E6 protein up-regulates the expression of the high mobility group protein HMG-I(Y) gene in mouse IOT1/2 cells" Virus Research. 42. 119-125 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Akutsu et al.: "p53-dependent and-independent traus-activation by the E6 protein of human papillomavirus type 16" Journal of General Virology. 77. 459-463 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shimizu et, al.: "Experssion,regulation and polymorphism of the mxil genes." Gene. 176. 45-48 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Shiga et al.: "Normal human fibroblasts can be immortalized by introduction of human papillomavirus type 16(HPV-16) E6-E7 genes" Microbiology and Immunology. in press.

    • Related Report
      1996 Annual Research Report
  • [Publications] Kinoshita et al.: "Transactivotion of prothy mosin-α and c-myc promoters by human papillomavirus type 16 E6 protein" Virology. in press.

    • Related Report
      1996 Annual Research Report
  • [Publications] Shino et al.: "Human papillomavirus type 16 E6 protein transcriptionally madulatec fibronactin gene expression by induction of protein complexes" Journal of Virology. (in press).

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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