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Studies on helminthotoxicity and host morbidity by eosinophils from IL-5 transgenic mice in Angiostrongylus cantonensis infection

Research Project

Project/Area Number 08670274
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 寄生虫学(含医用動物学)
Research InstitutionAKITA UNIVERSITY

Principal Investigator

YOSHIMURA Kentaro  Akita University, School of Medicine, Professor, 医学部, 教授 (90053058)

Co-Investigator(Kenkyū-buntansha) SHIMADA Hiroko (SUGAYA H)  Akita University, School of Medicine, Research Associate, 医学部, 助手 (30235626)
ISHIDA Kazuto  Akita University, School of Medicine, Research Associate, 医学部, 助手 (60006731)
ABE Tatsuya  Akita University, School of Medicine, Associate Professor, 医学部, 教授 (80128363)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsAngiostrongylus cantonensis / eosinophil / IL-5 transgenic mice / C.B-17 scid / BALB / c nu / nu / diffusion chamber / IgA / CD4^+T cell / 広東住血線虫 / IL-5 transgenic mouse / C.B.-17 scid / diffusion chamber / CD8^+T細胞 / 髄液 / major basic protein / eosinophil peroxidase / eosinophil cationic protein / eosinophil-derived neurotoxin
Research Abstract

This study demonstrated : 1. Angiostrongylus cantonensis worms in the subarachnoid spaces of IL-5 transgenic mice (IL-5 Tg) were surrounded by numerous eosinophils which degranulated and discharged lysosomal materials on to the worms, leading to worm killing. 2. Antigen-specific serum and cerebrospinal fluid (CSF) IgA antibodies were detectable on days 5 and 7 postinfection onward, respectively, in both IL-5 Tg and C3H/HeN mice, although the antibody level was higher in IL-5 Tg mice. 3. Anti-CD4 antibody treatment interfered with worm killing in BALB/c mice and improved host morbidity, whereas anti-CD8 antibody treatment failed to improve the morbidity. 4. Tumor necrosis factor-alpha does not cause morbidity of infected mice. 5. Anti-IL-5 antibody treatment blocked worm killing but failed to improve host morbidity. 6. Crude eosinophil basic proteins from IL-5 Tg was helminthotoxic to day 20 worms in vitro. 7. Both infected C.B-17 +/+ (+/+) and BALB/c nu/+ (nu/+) showed high morbidity, … More while both C.B-17 scid (SCID) and BALB/c nu/nu (nu/nu) failed to do so. 8. Intracranial worm recovery was markedly higher in +/+, SCID and nu/nu mice than in nu/+ and C57BL/6 mice. The former three strains, especially SCID and nu/nu, failed to show significant CSF eosinophilia. 9. Both SCID and nu/nu mice failed to produce a significant amount of antigen specific serum IgA and IgG1.10. Infected SCID mice previously transferred with peritoneal eosinophils from IL-5 Tg and also with serum IgA from infected IL-5 Tg yielded almost the same worm recovery as those transferred with eosinophils and the normal serum. This indicated that the accumulation of eosinophils in CSF occurs in nu/nu but not in SCID.11. When naive IL-5 Tg and C3H/HeN were implanted with a diffusion chamber (DC) containing infective third stage larvae (L3), cellular migration occurred in a DC with 2mum filter membrane, and the migration was more prominent in IL-5 Tg than in C3H/HeN.L3 larvae survived better in a DC with 0.1mum filter membrane than in a DC with 2mum membrane. Less

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (27 results)

All Other

All Publications (27 results)

  • [Publications] Yoshimura, K.: "Eosinophils intravenously transferred into Angiostrongylus cantonensis infected nude mice Kill intracranial worms in vivo." Acta Parasitol Turcica. Suppl 1. 281-291 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sugaya, H.: "Mediastinal lymph node cells of Angiostrongylus cantonensis-infected rats respond to antigens and release interleukin-5 in vivo." International Journal of Parasitology. 26. 543-551 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshida, T.: "Defective B-1 cell development and impared immunity against Angiostrogylus cantonensis in IL-5α-deficient mine." Immunity. 4. 483-494 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sugaya, H.: "Cytokine responses in mice infected with Angiostrongylus cantonensis." Parasitology Research. 83. 10-15 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sugaya, H.: "Eosinophilia and intracranial worm recovery in interleukin-5 transgenic and interleukin-5 receptar α chain-knockout mice infected with Angiostrogylus cantnensis." Parasitology Researc. 83. 583-590 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sugaya, H.: "Induction of crerbrospinal fluid eosinophilia in rats by the intraventricular injection of Angistrongylus cantonensis antigen." International Journal of Parasitology. 27. 113-117 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 菅谷 博子: "広東住血線中感染マウスにおけるTh2サイトカイン応答の優位性" 医学のあゆみ. 183. 249-252 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Aoki, M.: "The role of CD4' and CD8'T-cells in host morbidity and innate resistance to Angiostrongylus cantonensis in the mouse." Parasitology Researc. 84. 91-99 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshimura, K.: "Cox FEG,Kreier JP,Wakelin D(eds)Topley&Wilson's Microbilogy and Microbial Intections.9th edn.Vol.5.Parasitology,Arnold London" Angiostrongylus(Parastrongylus)and less common nematodes, 25 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshimura, K.: "Eosinophils intravenously transferred into Angiostrongylus cantonensis infected nude mice kill intracranial worms in vivo." Acta Parasitol Turcica. 20 (Suppl 1) (Proceedings). 281-291 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sugaya, H.: "Mediastinal lymph node cells of Angiostrongylus cantonensis-infected rats respond to antigens and release interleukin-5 in vitro." Int J Parasitol. 26. 543-551 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshida, T.: "Defective B-1 cell development and impaired immunity against Angiostrongylus cantonensis in IL-5Ralpha-deficient mice." Immunity. 4. 483-494 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sugaya, H.: "Cytokine responses in mice infected with Angiostrongylus cantonensis." Parasitol Res. 83. 10-15 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sugaya, H.: "Eosinophilia and intracranial worm recovery in interleukin-5 transgenic and interleukin-5 receptor alpha chainknockout mice infected with Angiostrongylus cantonensis." Parasitol Res.83. 583-590 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sugaya, H.: "Induction of cerebrospinal fluid eosinophilia in rats by the intraventricular injection of Angiostrongylus cantonensis antigen." Int J Parasitol.27. 113-117 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sugaya, H.: "Th2 dominant cytokine responses in murine Angiostrongylus cantonensis infection." Igaku No Ayumi. 183. 249-252 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Aoki, M.: "The roke of CD4^+ and CD8^+ T-cells in host morbidity and innate resistance to Angiostrongylus cantonensis in the mouse." Parasitol Res. 84. 91-99 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshimura, K.: "Angiostrongylus (Parastrongylus)and less common nematodes." Cox FEG,Kreier JP,Wakelin D (eds) Topley & Wilson's Microbiology and Microbial Infections. 9th edn.Vol.5. Parasitology, Arnold, London. 635-659 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sugaya, H.: "Eosinophilia and intracranial worm recovery in interleukin-5 transgenic and interleukin-5 receptor α chain-knockout mice infected with Angiostrongylus cantonensis." Parasitology Research. 83. 583-590 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 菅谷博子: "広東住血線虫感染マウスにおけるTh2サイトカイン応答の優位性" 医学のあゆみ. 183. 249-252 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Aoki,M.: "The role of CD4^+ and CD8^+ T-cells in host morbidity and innate resistance to Angiostrongylus cantonensis in the mouse." Parasitology Research. 84. 91-99 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yosimura, K.: "Cox FEG,Kreier JP,Wakelin D(eds)Topley & Wilson‘s Microbiology and Microbial Infections.9th edn.Vol.5.Parasitology,Arnold,London" Angiostrongylus(Parastrongylus)and less common nematodes, 25 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sugaya,H.: "Mediastinal lymph node cells of Angiostrongylus cantonensis-infected rats respond to antigens and release interleukin 5 in vitro." International Journal for Parasitology. 26. 543-551 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yoshida,T.: "Defective B-1 cell development and impaired immunity against Angiostrongylus cantonensis in IL-5 R α-deficient mice." Immunity. 4. 483-494 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yoshimura,K.: "Eosinophils intravenously transferred into Angiostrongylus cantonensis infected nude mice kill intracranial worms in vivo." Acta Parasitologia Turcica. Suppl.1. 281-291 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sugaya,H.: "Cytokine responses in mice infected with Angiostrongylus cantonensis." Parasitology Research. 83. 10-15 (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sugaya,H.: "Induction of cerebrospinal fluid eosinophilia in rats by intraventricular injection of Angiostrongylus cantonensis antigen." International Journal for Parasitology. 27(1). 113-117 (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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