| Project/Area Number |
08670286
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
寄生虫学(含医用動物学)
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| Research Institution | OSAKA CITY UNIVERSITY |
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Principal Investigator |
KIMURA Masatsugu Osaka City University, Medical School Assistant Prof,, 医学部, 助手 (60195378)
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| Co-Investigator(Kenkyū-buntansha) |
OGURA Hisashi Osaka City University, Medical School Prof., 医学部, 教授 (10115222)
TANABE Kazuyuki Osaka Institute of Technology, Prof., 教授 (40047410)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | Plasmodium falciparum / MSPl / Antigenic Polymorphism / Serology / Elisa / Expression / Antibody |
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| Research Abstract |
Block 2 of MSP1 (Merozoite Surface Protein 1) in plasmodium falciparum is a main polymorphic region of the protein, which can be divided into 3 basic allelic forms. We expressed the block 2 region in E.coli and purified large amount of the peptides for the three types.
2.Two rabbits were immunized with each peptide. Raised antisers recognized the same type of MSP1 protein but did not other types on Western blotting. Improving the Elisa method, we showed that each antiserum had a very high titer and did not react with the other types of peptide antigens much. Namely, Since each block 2 peptide expressed was highly antigenic and the induced antiserum for the peptide did not crossreact with other antigenic types, we concluded that these expressed peptides can be used for the analysis of human serum collected in malaria endemic areas.
3. We measured Elisa reactivity with three peptides to some 250 human sera which were collected in Southern part of Vietnam, and found that reactivity to these sera diverted greatly although these sera react well with crude extract of the parasite. Most of the samples had been analyzed for parasite infection and DNA typing for the parasite MSPl-block 2. We are now processing the data to extract the relationship between MSPl allelic forms and serum titers.
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