A study of relationship between Leptotrombidium species and serotypes of Orientia tsutsugamushi
| Project/Area Number |
08670293
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
寄生虫学(含医用動物学)
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| Research Institution | Niigata College of Pharmacy |
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Principal Investigator |
URAKAMI Hiroshi Niigata College of Pharmacy, Associate Professor, 薬学部 (80139732)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | Orientia tsutsugamushi / rickettsia / scrub typhus / vector / Leptotrombidium / Leptotrombidium intermedium / serotype / 細胞内共生 / 血清型 |
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| Research Abstract |
1, Immunofluorescent microscopy was developed to detect and characterize Orientia tsutsugamushi (Ot) in Leptotrombidium.
2, Although it was accepted that one family line of Leptotrombidium is infected simultaneously with several serotypes of Ot, we analyzed three family lines of Leptotrornbidium, and found that each was infected with a single serotype.
3, We found that 0.16% of L.intermedium was infected with Ot in an endemic area in Kyoto.
4, Ot was isolated from unfed larvae of L.intermedium, this is the first record from L.intermedium. Type specific antigenic protein gene (tsg) of the isolate was completely same to that of a strain isolated from a patient. This suggests that L.intermedium transmits Ot to humans.
5, Ot isolated in an area where L.pallidum does not exist had a tsg of the same sequence to that of Ot isolated from L.pallidum. This strongly suggested that Ot of the same serotype parasitized in more than one species.
6, We found a great diversity of tsg gene depending on locality from sequences of 34 strains isolated all over Asia. This suggests that Ot serotypes largely depends on Leptotrombidium species distributed in each area.
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Report
(3 results)
Research Products
(6 results)
