| Project/Area Number |
08670300
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Gunma University School of Medicine |
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Principal Investigator |
IYOBE Shizuko Gunma University School of Medicine, Associate Professor, 医学部, 助教授 (90008318)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Metallo-beta-lactamase / Carbapenemase / Integron / 緑膿菌 / β-lactamase遺伝子 / β-lactamase |
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| Research Abstract |
Resistant strains of Pseudomonas aeruginosa to BETA-lactams frequently produces carbapenem hydrolyzing metallo-BETA-lactamase, carbapanemase, which hydrolyzes almost all BETA-lactams. The molecular structure of the gene bla_<IMP> and mode of the dissemination in clinical isolates were examined
1. The gene bla_<IMP> was integrated as a cassette into the integron structure located on transmissible plasmid of clinical isolates of Pseudomonas aeruginosa.
2. The bla_<IMP> cassette had a specific 127 base-sequence at the terminal and deleted from the integron as a circular form of DNA with a 7 base-sequence of GTTRRRY.
3. The circular form of DNA was inserted back into the GTTRRRY sequence on the integron
4. The expression of bla_<IMP> was directed by the promoters located on the integraon
5. The bla_<IMP> was disseminated among clinical strains of Enterobactenaceae.
6. The gene bla_<MET> which coded for a variant metallo-BETA-lactamase with a narrow substrate-spectrum was isolated from a Shigella flexneri plasmid and the sequence was compared with that of the gene bla_<IMP>. Point mutations were identified in the gene bla_<MET>, suggesting the bla_<MET> to be an ancestor gene of bla_<IMP>
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