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PROMOTION OF PHAGOCYTOSIS AND PREVENTION OF PHAGOSOME-LYSOSOME (P-L) FUSION IN HUMAN PERIPHERAL BLOOD MONOCYTES BY SEROTYPE SPECIFIC GLYCOPEPTIDOLIPID (GPL) ANTIGEN OF MYCOBACTERIUM AVIUM COMPLEX (MAC)

Research Project

Project/Area Number 08670313
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Bacteriology (including Mycology)
Research InstitutionOSAKA CITY UNIVERSITY

Principal Investigator

TANO Ikuya  Osaka City Univ., Medical School Professor, 医学部, 教授 (60047008)

Co-Investigator(Kenkyū-buntansha) HAN Yuki  Osaka City Univ., Medical School Assistant, 医学部, 助手 (30271183)
HOTTA Hisako  Osaka City Univ., Medical School Assistant, 医学部, 助手 (00165002)
OKA Siro  Osaka City Univ., Medical School Associate Prof., 医学部, 助教授 (40160650)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
Keywordsphagocytosis / phago-lysosomefusion / virulence factor / glycopeptido lipid / cord-factor / serotype-4 GPL / Mycobacterium auium / M.tuberculosis / ファゴ・リソゾーム融合 / M.tuberculosis / 結核菌 / ファゴソーム・リソゾーム融合 / グリコペプチドリピド / スルホリピッド / 食細胞機能阻害
Research Abstract

Mycobacterium avium complex (MAC) is one of the most important pathogen coinfected with HIV (AIDS) and a typical intracellular parasite as well as M.tuberculosis. It is also focused that M.avium infection causes immunosuppression especially in the cellular immunity of host animals, and specific serotype-subspecies such as serotype-2, -4 or-8 can be isolated frequently in human infection in AIDS.Furthermore, the prognosis after infection differs by the serotype ; sero-4 shows heavy infection in general, while sero-16 shows rapid improvement. Therefore, we have been interested in the immunomodifying activity of surface glycopeptidolipid (GPL) antigen. However, to date, no information has been available on the virulent factor of MAC related directly with intracellular bacterial killing. Recently, we have tried to test the effect of various GPLs purified from MAC complex on phagocytic processes of human peripferal blood monocytes (PBMC). We have used GPL-coated heat-killed staphylococcal cells to be phagocytosed by PBMC,and P-L fusion was estimated by the acridine orange staining of fused vesicles and bacteria. As the results, serotype-4 GPL showed strong phagocytosis promotion and marked inhibition of P-L fusion, while serotype-16 GPL showed neither promotion of phagocytosis, nor inhibition of P-L fusion in phagocytic cells. Serotype-8 GPL showed stimulation of both phagocytosis and P-L fusion, concomitantly. These effects may be due to unknown interaction between specific carbohydrate chain and organella membranes, and serotype-4 GPL may be one of the possible virulent factors in MAC infection in human phagocytic cells. Comparison with known possible virulent factors such as cord factor, trehalose monomycolate or sulfolipids will be reported.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Seiko Yamaguchi: "Stimulatory effect of gangliosides on phagocytosis,phagosome-lysosome fusion,and intracellular signal transduction system by human polymophonuclear leukocytes" Glycoconjugate Journal. 14. 707-714 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 藤原 永年: "人型結核菌株間における抗原性糖脂質の分布とvirulenceへの寄与に関する研究" 結核. 72No.4. 21-33 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Seiko Yamaguchi: "Stimulatory effect of gangliosides on phagocytosis,phagosome-lysosome fusion,and intracellular signal transduction system by human polymorphonuclear leukocytes" Glycoconjugate Journal. 14. 707-714 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 藤原永年: "人型結核菌株間における抗原性糖脂質の分布とvirulenceへの寄与に関する研究" 結核. 72No.4. 21-33 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Seiko Yamaguchi et al.: "Stimulation of phagocytosis and phagosome-lysosome (P-L) fusion of human polymorphonuclear leukocytes by sulfatide (galactosylceramide-3-sulfate)" FEMS Immunology and Medical Microbiology. 13. 107-111 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yoshiko Miyazaki et al.: "Stimulation of phagocytosis and phagosome-Lysosome fusion by Glycosphingolopids from Sphingomonas paucimobilis" J.Biochem.118. 271-277 (1995)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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