PROMOTION OF PHAGOCYTOSIS AND PREVENTION OF PHAGOSOME-LYSOSOME (P-L) FUSION IN HUMAN PERIPHERAL BLOOD MONOCYTES BY SEROTYPE SPECIFIC GLYCOPEPTIDOLIPID (GPL) ANTIGEN OF MYCOBACTERIUM AVIUM COMPLEX (MAC)
| Project/Area Number |
08670313
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | OSAKA CITY UNIVERSITY |
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Principal Investigator |
TANO Ikuya Osaka City Univ., Medical School Professor, 医学部, 教授 (60047008)
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| Co-Investigator(Kenkyū-buntansha) |
HAN Yuki Osaka City Univ., Medical School Assistant, 医学部, 助手 (30271183)
HOTTA Hisako Osaka City Univ., Medical School Assistant, 医学部, 助手 (00165002)
OKA Siro Osaka City Univ., Medical School Associate Prof., 医学部, 助教授 (40160650)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | phagocytosis / phago-lysosomefusion / virulence factor / glycopeptido lipid / cord-factor / serotype-4 GPL / Mycobacterium auium / M.tuberculosis / ファゴ・リソゾーム融合 / M.tuberculosis / 結核菌 / ファゴソーム・リソゾーム融合 / グリコペプチドリピド / スルホリピッド / 食細胞機能阻害 |
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| Research Abstract |
Mycobacterium avium complex (MAC) is one of the most important pathogen coinfected with HIV (AIDS) and a typical intracellular parasite as well as M.tuberculosis. It is also focused that M.avium infection causes immunosuppression especially in the cellular immunity of host animals, and specific serotype-subspecies such as serotype-2, -4 or-8 can be isolated frequently in human infection in AIDS.Furthermore, the prognosis after infection differs by the serotype ; sero-4 shows heavy infection in general, while sero-16 shows rapid improvement. Therefore, we have been interested in the immunomodifying activity of surface glycopeptidolipid (GPL) antigen. However, to date, no information has been available on the virulent factor of MAC related directly with intracellular bacterial killing. Recently, we have tried to test the effect of various GPLs purified from MAC complex on phagocytic processes of human peripferal blood monocytes (PBMC). We have used GPL-coated heat-killed staphylococcal cells to be phagocytosed by PBMC,and P-L fusion was estimated by the acridine orange staining of fused vesicles and bacteria. As the results, serotype-4 GPL showed strong phagocytosis promotion and marked inhibition of P-L fusion, while serotype-16 GPL showed neither promotion of phagocytosis, nor inhibition of P-L fusion in phagocytic cells. Serotype-8 GPL showed stimulation of both phagocytosis and P-L fusion, concomitantly. These effects may be due to unknown interaction between specific carbohydrate chain and organella membranes, and serotype-4 GPL may be one of the possible virulent factors in MAC infection in human phagocytic cells. Comparison with known possible virulent factors such as cord factor, trehalose monomycolate or sulfolipids will be reported.
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Report
(3 results)
Research Products
(6 results)
