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Analysis of immortalization of normal T lymphocytes by Epstein-Barr virus

Research Project

Project/Area Number 08670332
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionHokkaido University

Principal Investigator

IMAI Shosuke  Hokkaido University School of Medicine, Cancer Institute, Department of Virology, Lecturer, 医学部, 講師 (60232592)

Co-Investigator(Kenkyū-buntansha) SUGIURA Makoto  Hokkaido University School of Medicine, Cancer Institute, Department of Virology, 医学部, 助手 (20241317)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
KeywordsEpstein-Barr virus / Tlymphocytes / immortalization / recombinant virus
Research Abstract

This study aimed to investigate the unclarified Epstein-Barr virus (EBV) activity on normal T-lymphocyte immortalization. For infection experiments, we used recombinant EBV (Akata strain) carrying the neomycin-resistance gene (neo-EBV). Although normal peripheral T lymphocytes could not be infected by neo-EBV,primary thymocytes revealed EBNA1 expression after virus exposure. However, the following selection culture did not yield permanently growing thymocytes infected with neo-EBV even in the presence of interleukin 2 (IL-2) in culture medium, probably because virus-infected thymocytes tended to enter into the virus lytic cycle. These results suggest that EBV infection of thymocytes results in the permissive, not latent, infection in vivo, which may be associated with EBV-induced T-lymphoproliferative disorders.
We are currently studying whether EBNA2, or-3A,or-3C gene can alter EBV-infected T-cell lines, the growth of which is dependent on IL-2 so far, such as in terms of aquisition of IL-2 independency or more malignant phenotype.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (31 results)

All Other

All Publications (31 results)

  • [Publications] Imai, S.: "Epstein-Barr virus-carrying and-expressing T-cell lines established fromsevere cheonic active EBV infection." Blood. 87. 1446-1457 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Harabuchi, Y.: "Nasal T-cell lymphoma causally associated with Epstein-Barr virus. Clinicopathologic, phenotypic, and genotypic studies." Cancer. 77. 2137-2149 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sugiura, M.: "Transcriptional analysis of Epstein-Barr virus gene expression in EBV-positive gastric carcinoma : unique viral latency in the tumour cells." Br.J.Canser. 74. 625-631 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Azuma, H.: "Hodgkin's disease developed in a patient with extremely high serum antibody titers against Epstein-Barr virus associated antigens without chronic illness." J.Pediatr.Hematol.Oncol.,. 18. 387-391 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kusuhara, K.: "Breast milk is not a signifivant source for early Epstein-Barr virus or human herpesvirus 6 infection in infants:a seropidemiologic study in 2 endemic areas of human T-cell lymphotropic virus type I in Japan." Microbiol.Immunol.41. 309-312 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sone, S.: "Enhanced cytokine production by milk macrophages following infection with respiratoriy syncytial virus" J.Leukocyte Biol.61. 630-636 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshiyama, H.: "Implication of the existence of anew virus receptor different from CD21." J.Virol.71(7). 5688-5691 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Harabuchi, Y.: "Nasal T-cell lymphoma causally associated with Epstein-Barr virus." Gann Monograph on Cancer Res.45. 129-137 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takeudhi, R.: "Respiratory syncytial virus infection of neonatal monocytes stimulates synthesis of interferon regulatory factor-1 (IRF-1) and IL-1 β converting enz-yme (ICE) to secrete IL-1β." J.Virol.(in press.).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Imai, S.: "Cell-to-cell contact as an efficient mode of Epstein-Barr virus infection of diverse human epithelial cells." J.Virol.(in press.).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Imai, S., Sugiura, M., Oikawa, O., Koizumi, S., Hirao, M., Kimura, H., Hayashibara, H., Terai, N., Tsutsumi, H., Oda, T., Chiba, S., Osato, T.: "Epstein-Barr virus-carrying and-expressing T-cell lines established from severe chronic active EBV infection." Blood. 87. 1446-1457 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Harabuchi, Y., Imai, S., Wakashima, J., Hirao, M., Kataura, A., Osato, T., Kon, S.: "Nasal T-cell lymphoma causally associated with Epstein-Barr virus. clinicopathologic, phenotypic, and genotypic studies." Cancer. 77. 2137-2149 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sugiura, M., Imai, S., Tokunaga, M., Koizumi, S., Uchizawa, M., Okamoto, K., Osato, T.: "Transcriptional analysis of Epstein-Barr virus gene expression in EBV-positive gastric carcinoma. unique viral latency in the tumour cells." Br.J.Cancer. 74. 625-631 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Azuma, H., Sakata, Y., Sasaki, K., Oka, T., Hironaka, T., Hirai, K., Imai, S., Osato, T., Okuno, A.: "Hodgkin's disease developed in a patient with extremely high serum antibody titers against Epstein-Barr virus associated antigens without chronic illness." J.Pediatr.Hematol.Oncol.18. 387-391 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kusuhara, K., Takabayashi, A., Ueda, K., Hidaka, Y., Minamishima, I., Take, H., Fujioka, K., Imai, S., Osato, T.: "Breast milk is not a signifivant source for early Epstein-Barr virus or human herpesvirus 6 infection in infants : a seroepidemiologic study in 2 endemic areas of human T-cell lymphotropic virus type I in Japan." Microbiol.Immunol.41. 309-312 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Sone, S., Tsutsumi, H., Takeuchi, R., Matsuda, K., Imai, S., Ogra, P.L., Chiba, S.: "Enhanced cytokine production by milk macrophages following infection with respiratory syncytial virus." J.Leukocyte Biol.61. 630-636 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshiyama, H., Imai, S., Shimizu, N., Takada, K.: "Epstein-Barr virus infection of human gastric carcinoma cells : Implication of the existence of a new virus receptor different from CD21." J.Virol.71(7). 5688-5691 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Harabuchi Y,Imai S,Kataura A,Osato T,Kon S: "Nasal T-cell lymphoma causally associated with Epstein-Barr virus." Gann Monograph on Cancer Res.45. 129-137 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takeuchi R,Tsusumi H,Osaki M,Sone S,Imai S,Chiba S: "Respiratory syncytial virus infection of neonatal monocytes stimulates synthesis of interferon regulatory factor-1 (IRF-1) and IL-1beta converting enzyme (ICE) to secrete IL-1beta." J.Virol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Imai, S., Nishikawa, J., Takada, K.: "Cell-to-cell contact as an efficient mode of Epstein-Barr virus infection of diverse human epithelial cells." J.Virol.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kusuhara,K.: "Breast milk not a signifivant source for early Epstein-Barr virus or human herpesvirus 6 infection in infants : a seroepidemiologic study in 2 endemic areas of human T-cell lymphotropic virus type I in Japan" Microbiol,Immunol.41. 309-312 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Sone,S.: "Enhanced cytokine production by milk macrophages following infection with respiratory syncytial virus" J.Leukocyte Biol.61. 630-636 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yoshiyama,H.: "Implication of the existence of a new virus receptor different from CD21." J.Virol.71(7). 5688-5691 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Harabuchi,Y.: "Nasal T-cell lymphoma causally associated with Epstein-Barr virus." Gann Monograph on Cancer Res.45. 129-137 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takeuchi,R.: "Respiratory syncytial virus infection of neonatal monocytes stimulates synthesis of interferon regulatory factor-1-(IRF-1) and IL-1β converting enzyme (ICE) to secrete IL-1β." J.Virol.(in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Imai,S.: "Cell-to-cell contact as an efficient mode of Epstein-Barr virus infection of diverse human epithelial cells." J.Virol.(in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Imai, S., et al.: "Epstein-Barr virus-carrying and -expressing T-cell lines established from severe chronic active EBV infection." Blood. 87-4. 1446-1457 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Harabuchi, Y., et al.: "Nasal T-cell lymphoma causally associated with Epstein-Barr virus : clinicopathologic, phenotypic, and genotypic studies." Cancer. 77-10. 2137-2149 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sugiura, M., et al.: "Transcriptional analysis of Epstein-Barr virus gene expression in EBV-positive gastric carcinoma : unique viral latency in the tumour cells." Br J Cancer. 74. 625-631 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Osato, T., Imai, S.: "Epstein-Barr virus and gastric carcinoma." Seminars in Cancer Biology. 7. 175-182 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Sone, S., et al.: "Enhanced cytokine production by milk macrophages folloeing infection with respiratory syncytial virus." J Leukocyte Biol. (in press).

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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