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STUDY ON THE MODE OF ACTION OF MONOCLONAL NONSPECIFIC SUPPRESSOR FACTOR (MNSF)

Research Project

Project/Area Number 08670367
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Immunology
Research InstitutionSHIMANE MEDICAL UNIVERSITY

Principal Investigator

NAKAMURA Morihiko  SHIMANE MEDICAL UNIVERSITY BIOCHEMISTRY,ASSISTANT, 医学部, 助手 (20155865)

Co-Investigator(Kenkyū-buntansha) TANIGAWA Yoshinori  SHIMANE MEDICAL UNIVERSITY,BIOCHEMISTRY,PROFESSOR, 医学部, 教授 (60084860)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsnonspecific / suppressor factor / T cell receptor / サンプレッサー因子 / ユビキチン
Research Abstract

Hybridoma-derived monoclonal nonspecific suppressor factor (MNSF) was purified with the use of anti-MNSF monoclonal antibody. SDS-polyacryl amide gel electrophoresis of the purified native MNSF was performed under reducing conditions, followed by immunoblot transfer and chemical staining with Amino black. The 70-kDa band was excised, and subjected to amino acid sequence analysis. The N-terminal sequence of 18 amino acids was determined. Degenerated primers corresponding to five amino acids were synthesized and used for RF-PCR.MNSFalpha mRNA was expressed in concanavalin A (Con A) activated (12 hours ) -, but not in unstimulated- T cells. The amino acid sequences deduced from the partial MNSFalpha cDNA showed no homology to any known proteins. However, the sequence showed 35% homology to T cell receptor (TCR) alpha chain in good aggrement with a hypothesis that MNSFalpha might be related to TCRalpha chain. The sequencing of full MNSFalpha cDNA was under way. The elucidation of the mode of action of MNSF with the use of recombinant MNSFalpha should be possible, in the near future.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Morihiko Nakamura: "Ubiquitin-like polypeptide inhibits the IgE response of lipopolySacckaride-activated B cells" International Immunology. 8. 1659-1665 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Morihiko Nakamura: "Ubiquitin-like polypeptide conjugates to acceptor proteins in concanavalin A-and interferon r-stimulated T-cells" Biochemical Journal. 330. 683-688 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tomoko Nagata: "Conjugation of Ubiquitin-like polypeptide to intracellular acceptor proteins" Biochimica et Biophysica Acta. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yuko Nariai: "Production and characterization of a monoclonal antibody specific for ubiquitin-like polypeptide" Journal of Immunoassay. (in press). (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Morihiko Nakamura, Tomoko Nagata: "Ricardo Xavier and Yoshinori Taanigawa Ubiquitin-like polypeptide inhibits the IgE response of lipopolysaccaride activated B cells." International Immunology. 8. 1659-1665 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Morihiko Nakamura and Yoshinori Tanigawa: "Ubiquitin-like polypeptide conjugates to acceptor proteins in concanavalin A and interferon gamma stimulated T-cells." Biochemical Journal. 330. 683-688 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Tomoko Nagata, Morihiko Nakamura, Hideyuki Kawauchi and Yoshinori Tanigawa: "Conjugation of ubiquitin-like polypeptide to intracellular acceptor proteins." Biochimica et Biophysica Acta. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yuko Nariai, Morihiko Nakamura, Tomoyoshi Kondoh and Yoshinori Tanigawa: "Production and characterization of a monoclonal antibody specific for ubiquitin-like polypeptide." Journal of Immunoassay. (in pres). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Morihiko Nakamura: "Ubiquitin-like polypeptide inhibits the IgE response of lipopolysaccharide-activated B cells." International Immunology. 8. 1659-1665 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Morihiko Nakamura: "Ubiquitin-like polypeptide conjugates to acceptor proteins in Concanavalin A-and interferon C-Stimulated T-cells." Biochemical Journal. 330. 683-688 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Tomoko Nagata: "Conjugation of ubiquitin-like polypeptide to intracellular acceptor proteins." Biochimica et Biophysica Acta. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Yuko Nariai: "Production and characterization of a monoclonal antibody specific for ubiquitin-like polypeptide" Journal of Immunoassay. (in press). (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Morihiko Nakamura: "Ubiquitin-like moiety of the monoclonal nonspecific suppressor factorβ(MNSF)is responsible for its activity" The Journal of Immunology. 156. 533-538 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Morihiko Nakamura: "Ubiquitin-like polypeptide inhibits the IgE response of lipopolysaccharide-activated B cells" International Immunology. 8. 1659-1665 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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