A follow-up study for planning preventive procedures of osteoporosis based on genetic and lifestyle risk assessment

• Project/Area Number: 08670419
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Public health/Health science
• Research Institution: Kinki University School of Medicine (1997); 福井医科大学 (1996)
• Principal Investigator: IKI Masayuki Kinki Univ.School of Medicine Department of Public Health Professor, 医学部, 教授 (50184388)
• Co-Investigator(Kenkyū-buntansha): DOHI Yoshiko Nara Medical University Department of Public Health Associate Professor, 医学部, 助教授 (50155628)
• Project Period (FY): 1996 – 1997
• Project Status: Completed (Fiscal Year 1997)
• Budget Amount: ¥2,100,000 (Direct Cost: ¥2,100,000); Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000); Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
• Keywords: Bone mineral density / Estrogen receptor genotype / Lifestyle factors / Osteocalcin genotype / Osteoporosis / Preventive procedures / Vitamin D receptor genotype / 第一次予防対策 / 遺伝要因 / Vitamin D受容体遺伝子 / Estrogen 遺伝子 / RFLP / ライフスタイル / リスク評価

Research Abstract

To establish effective procedures for the prevention of osteoporosis, we evaluated the effects of polymorphisms of several genes relating to bone metabolism and the effects of various lifestyle factors on bone mineral density of the lumbar spine and on its change during the follow-up period. The results obtained were as follows :
(1)Vitamin D receptor genotype showed a significant effect on the bone mineral density at baseline after the adjustment of the confounding effects of age, menopausal status, height and weight.
(2)Estrogen receptor genotype had relatively smaller but still significant effect on the bone mineral density at baseline independently from the effect of vitamin D receptor genotype.
(3)We found a novel polymorphism in the promoter region of the osteocalcin gene by single strand conformation polymorphism technique. We established a procedure to detect a restriction fragment length polymorphism of this site of the gene using a restriction enzyme Hind III.The homozygote of the mutant allele of this polymorphism was suggested to have a significantly greater risk for the development of osteopenia.
(4)These polymorphism exhibited significant effects on bone mineral density at baseline while no significant effect was observed on the change in bone density during the follow-up.
(5)Greater dietary calcium intake and greater muscle strength of the trunk had a significant effect in increasing bone density and smoking habits showed a decreasing effect in postmenopausal women.
Genetic factors may play a great role in acquiring the peak bone mass but not in determining the amount of postmenopausal bone loss. Preventive procedures for osteoporosis allowing for the genetic risk might be applied on younger people.

Research Products

• [Publications] Iki M, Dohi Y, et al.: "Relative contributions of age and menopause to the rertebral bone density of health Japanese women." Bone. 18. 617-620 (1996)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Iki M, Kajita E, et al.: "Age,Menopause,bone turnover markers and lumbar bone loss in healthy Japanese women." Maturitas. 25. 59-67 (1996)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Iki M, at al.: "Vitamin D receptor genotype and lumbar bone density in healthy Japanese women" Osteoporos Jpn. (in Japanese with English abstract). 211-215 (1996)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 伊木 雅之: "骨粗鬆症への疫学的アプローチ、地域を対象とした一次予防" Clin Calcium. 6. 861-864 (1996)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Iki M, et al.: "Greater muscle torque of the trunk extensors prevents postmenopausal bone loss in the lumbar spine." Proceedings of IRMA VIII. 1205-1209 (1997)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Dohi Y, Iki M, et al.: "A novel polymorphism in the promoter region for the human osteocalcin gene The possibility of a correlation with bone mineral density in post menepausal Japanese women" J Bone Miner Res. 13 (in press). (1998)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Iki M,Dohi Y,et al: "Relative contributions of age and menopause to the vertebral bone density of health Japanese women" Bone. 18. 617-620 (1996)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Iki M,Kajita E,et al: "Age, Menopause, bone turnover markers and lumbar bone loss in healthy Japanese women" Maturitas. 25. 59-67 (1996)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Iki M,et al.: "Vitamin D receptor genotype and lumbar bone density in healthy Japanese women. (in Japanese with English abstract)" Osteoporos Jpn. 211-215 (1996)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nishino H,Tanaka T,Horii H,Kumanishi S,Iki M,et al.: "Relation between parameters of bone mass by ultrasound system and bone mineral density of lumbar spine or each biochemical marker of bone metabolism. (in Japanese)" Annual Rep Toyama Inst Health. 19. 138-147 (1996)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Iki M: "Primary prevention procedures for osteoporosis on community basis. (in Japanese)" Clin Calcium. 6. 861-864 (1996)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Iki M,et al.: "Greater muscle torque of the trunk extensors prevents postmenopausal bone loss in the lumbar spine" In : Ueda S,Nakamura R,.Ishigami S,eds. Proceedings of IRMA VIII.Monduzzi Editore, Bologna, Italy. 1205-1209 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nakata K,Takagi H,Tamaki S,Takayama S,Iki M,et al.: "Functional prognosis after surgical treatment of hip fracture in the elderly" In : Ueda S,Nakamura R,Ishigami S,eds.Proceedings of IRMA VIII.Monduzzi Editore, Bologna, Italy. 1261-1265 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Nishino H,Tanaka T,Horii H,Kumanishi S,Iki M,et al.: "Association between change of habitual exercise and that of bone biochemical markers in healthy postmenopausal women. (in Japanese)" Annual Rep Toyama Inst Health. 20. 152-158 (1997)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Dohi Y,Iki M,et al.: "A novel polymorphism in the promoter region for the human osteocalcin gene : The possibility of a correlation with bone mineral density in postmenopausal Japanese women." J Bone Miner Res. (in press).(1998)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Iki M, et al.: "Greater muscle torque of the trunk extensors prevents postmenopausal bone loss in the lumbar spine." Proceedings of IRMA VIII. 1205-1209 (1997)
• [Publications] Nakata K, Iki M, et al.: "Functional prognosis after surgical treatment of hip fracture in the elderly." Proceeding of IRMA VIII. 1261-1265 (1997)
• [Publications] 西野治身, 伊木雅之, 他: "健常女性における踵骨超音波計測値と腰椎骨密度ならびに骨代謝指標との関係" 富山衛生研究所年報. 20. 152-158 (1997)
• [Publications] Dohi Y, Iki M, et al.: "A novel polymorphism in the promoter region for the human osteocalcin gene : The possibility of a correlation with bene mineral density in pestmenepausal Japanese women." J Bone Miner Res. 13(in press). (1998)