| Project/Area Number |
08670471
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Public health/Health science
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| Research Institution | Osaka Prefectural Institute of Public Health |
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Principal Investigator |
HAMANO Yonekazu Osaka prefectural Institute of Public Health, Dept.Food Santation, Chief Researcher, 食品衛生部, 主任研究員 (70159094)
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| Co-Investigator(Kenkyū-buntansha) |
KAWATSU Kentaro Osaka Prefectural Institute of Public Health, Dept.Food Sanitation, Researcher, 食品衛生部, 研究員 (20260367)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1997: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | ELISA / diarrhetic shellfish toxins / monoclonal antibody / bibalvia / ELISA / 乳のみマウス法 |
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| Research Abstract |
An enzyme linked immunosorbent assay (ELISA) for the quantitative analysis of okadaic acid (OA) and it's derivatives (DTX-1, DTX-3) from shellfish extracts and freshwater plankton samples has been developed. This ELISA (direct non-competitive assay) utilizes a monoclonal antibody against OA as a capture antibody, which is tolerant to 50% or less aqueous organic solvent like methyl alcohol and acetone, and a peroxidase-conjugated okadaic acid as detecting residual sites of the antibody. The working range for quantitative analysis ranged from 20 ng/ml to 150 ng/ml in the extracts, and the detection limit was 0.02 MU/g in original sample. recoveries of OA,DTX-1 and DTX-3 by this ELISA were >95% for scallop. Moreover, this assay brings the results within 3 hours containing the sample preparation.
In conclusion, this ELISA method is sensitive, accurate, reproducible, simple and rapid to carry out, without using expenxive equipment. This assay should be useful in the monitoring assessment of diarrhetic shellfish toxins.
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