A new analytical method using a new reversed-phase chromatographic columnn a 2-mum porous microspherical silica gel.
| Project/Area Number |
08670476
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | University of Tsukuba |
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Principal Investigator |
TANAKA Einosuke University of Tsukuba, Institute of Community Medicine, Lecturer, 社会医学系, 講師 (30138416)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1997: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | High-performance liquid chromatography / Biological sample / Silica gel / Forensic Toxicology / Benzodiazepine drugs / ベンゾジアゼピン薬剤 / 2-ミクロン充填剤 |
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| Research Abstract |
Recently, a new reversed-phase chromatographic column, TSK gel Super-ODS,based on 2-mum silica gel, became commercially available from Toshoh (Tokyo, Japan). This ODS colum achieves better resolution and fast separation than that of conventional ODS column (particle size 5mum). We report the simultaneous determinaition of antiepireptic drugs and benzodiazepine drugs in human serum using this reversed-phase column in comparison with the conventional ODS column in 1995 and 1996, respectively.
A high-performance liquid chromatographic method has been developed for the forensic analysis of cyclic antidepressant drugs in 1997.
This ODS column packing has the following advantages : (1) more rapid determination can be expected at room temperature, even without gradient elution compared with the conventional column packing, (2) the amount of organic solvent required is smaller and (3) more sensitive determination is possible.
These results show that a new ODS filler with a particle size of 2mum was more sensitive, provided better separation, and was more rapid than with conventional ODS filler.
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Report
(3 results)
Research Products
(7 results)
