| Project/Area Number |
08670518
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | Okayama University |
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Principal Investigator |
YAMAMURA Masahiro Okayama University Medical School, Assistant Professor, 医学部・附属病院, 講師 (80252956)
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| Co-Investigator(Kenkyū-buntansha) |
SHIKATA Ken-ichi Okayama University Medical School, Assistant Professor, 医学部・附属病院, 講師 (00243452)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | interferon-gamma / interleukin-12 / macrophages / osteoarthritis / rheumatoid arthritis / T cells / Th1 cells / Th1型反応 |
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| Research Abstract |
The importance of interleukin-12 as a factor in the interferon-gamma (IFN-gamma) -dominant T cell cytokine response in the synovial tissue of patients with rheumatoid arthritis (RA) was examined. Synovial tissues from RA patients more strongly expressed IL-12 p40 mRNA than did osteorthritis (OA) tissues, as determined by RT-PCR.Dissociated tissue cells from RA patients sspontaneously released detectable amounts of IL-12 p70 in culture, whereas production of IL-12 by OA tissues was limited. By immunohistochemical analysis, IL-12-producing cells were localized mainly in the sublining layr of RA synovium, and mostly expressed the CD68 antigen. Levels of IFN-gamma production by RA synovial tissue cells were potently and selectively enhanced by IL-12. The ability of IL-2-expanding synovial T cells to produce IFN-gamma was augmented by costimulation with IL-12 and diminished by anti-IL-12, while it was not affected by IL-4. These dada suggest that IL-12, produced mainly by macrophage-lineage cells, may be involved in IFN-gamma dominant cytokine production by infiltrating T cells in joints with chronic RA.
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