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PATHOGENETIC SIGNIFICANCE OF PROTEIN KINASE JNK-1 INDUCED BY ULTRAVIOLET IN PERIPHERAL BLOOD LYMPHOCYTES FROM SLE PATIENTS

Research Project

Project/Area Number 08670529
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionSAITAMA MEDICAL SCHOOL

Principal Investigator

AMANO Koichi  SAITAMA MEDICAL SCHOOL,SCHOOL OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 講師 (00175928)

Co-Investigator(Kenkyū-buntansha) TAKEUCHI Tsutomu  SAITAMA MEDICAL SCHOOL,SCHOOL OF MEDICINE,PROFESSOR, 医学部, 教授 (50179610)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsUltraviolet / c-Jun N-terminal kinase-1 (JNK-1) / systemic lupus erythematosus (SLE) / lymphocytes / signal transduction / apoptosis / 光線過敏症
Research Abstract

Objects and Methods : Peripheral blood lymphocytes (PBL) were obtained from lOSLE patients and 5 normal healthy volunteers (NHV). Five mililiter of lx 10^6/ml PBL was transfered to the Petri dish and UV-irradiated for 20 seconds (wave length 302 nm, 8 mW/cm^2) Non-irradiated PBL were used as the control. (1) The levels of IgG, IL-2 & IL-6 were measured by ELISA after 7 day-culture of PBL.(2) The ratio of apoptotic cells was determinied by using the monoclonal antibodies (apo.2.7 or anti- annexin 5 antibody). (3) The serine-phosphorylation of JNK-1 was investigated by Western blot technique after PBL were lysed with NP-40.
Results : (1) The production of IgG, IL-2 and IL-6 was increased after UV-irradiation in SLE, but not in NHV.(2) After UV irradiation, about 75% of PBL were positive for apo 2.7 or annexin 5 in NHV whereas 95% of PBL were apoptotic in SLE.(3) The serine-phosphorylation of JNK-1 was more apparent after UV irradiation in SLE than in NHV.
Discussion : According to my data, there can be a speculation that the immunosuppressive functional subpopulation of SLE-PBL was senseitive to apoptotic stimuli such as UV-irradiation and then SLE-PBL, as a whole, was activated to produce cytokines and IgG after irradiation. And the serine-phosphorylation of JNK-1 could be involved in PBL activation via UV stimulation. There might be a defect in immunosuppressive functional subpopulation of SLE-PBL.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Takeuchi T et.al.: "Upregulated expression and function of integrin adhesive receptor in SLE patients with vasculitis" J.Clin.Invest. 92. 3008-3016 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takeuchi T et.al.: "Reduced tyrosine phosphatase activity of CD45 molecule on periphcral T cells in patients with systemic lupus erythematosus" Arthritis Rheum. 36. S.146 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takeuchi T et.al.: "Aberrant expression of VLA-4 antigen in systemic lupus erythematosus patients with vasculitis" Arthritis Rheum. 36. S71 (1993)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Amano K et.al.: "Unusual variant of Wegener's granulomatosis and the role of integrin adhesion receptor for pulmonary lesion" Jap J.Rheumatol. 5. 217-226 (1994)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takeuchi T et.al.: "Reduced protein tyrosine phosphaiase (PTP ase) activity of CD45 on peripheral blood lymphocytes in patients with SLE" Clin Exp Immunol. 109. 20-26 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Pang M.et.al.: "Up-regulation of αEβ7, a novel integrin adhesion molecule on T cells from systemic lupuserythematosus patients with sperificepithelial involvement" Arthritis Rheum. 41. 1456-1463 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 天野宏一、他: "リウマチ性疾患治療の新展開" 医薬ジャーナル社, (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takeuchi, T., Amano, K., Abe, T., et al.: "Upregulated expression and function of integrin adhesive receptor in SLE patients with vasculitis" J.Clin.Invest.92. 3008-3016 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takeuchi, T., Amano, K., Abe, T., et al.: "Redused tyrosine phosphatase activity of CD45 molecule on peripheral T cells in patients with systemic lupus erythematosus" Arthritis Rheum.36. S146 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takeuchi, T., Amano, K., Abe, T., et al.: "Aberrant expression of VLA-4 antigen in systemic lupus erythematosus patients with vasculitis" Arthritis Rheum.36. S71 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Amano, K., Takeuchi, T., Abe, T., et al.: "Unusual variant of Wegener's granulomatosis and the role of integrin adhesion receptor for pulmonary lesion" Jap.J.Rheumatol.5(3). 217-226 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takauchi T., Pang M., Amano K., et al.: "Reduced protein tyrosine phosphatase (PTPase) activity of CD45 on peripheral blood lymphocytes in patients with systemic lupus erythematos us (SLE)" Clin Exp Immunol. 109. 20-26 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Pang M., Amano K., Takeuchi T., et al.: "Up-regulation of alphaEbeta7, a novelintegrin adhesion molecule, on T cells from systemic lupus erythematos us patients with specific epithelial involvement" Arthritis Rheum. 41. 1456-1463 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary

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Published: 1996-04-01   Modified: 2016-04-21  

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