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Clinical and pathological significance of autoantibodies against tRNA and tRNA-associated proteins

Research Project

Project/Area Number 08670533
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionKEIO UNIVERSITY

Principal Investigator

OHOSONE Yasuo  KEIO UNIVERSITY SCHOOL OF MEDICINE,INSTRUCTOR, 医学部, 助手 (20160492)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsAUTOANTIBODIES / tRNA / Systemic lupus erythematosus / Sjogren's syndrome / シェ-グレン症候群 / 全身性エリテマトーデス
Research Abstract

Autoantibodies directed against aminoacyl-tRNA synthetases are well described in adult polymyositis/dermatomyositis. However, the characteristics of antibodies against other tRNA and tRNA-associated proteins have not been well defined. In this study, we identified novel autoantibodies to total tRNAs in patients with systemic rheumatic diseases and characterized the structure that was recognized by anti-tRNA antibodies.
We identified fifteen patients sera that immunoprecipitated the deproteinized cognate tRNA.Two of them were identified as having previously well-defined anti-PL-12 antibodies. Three of the remaining 13 patient sera immunoprecipitated naked tRNA of E.coli. To investigate the antibody binding site on tRNAs, we have used in vitro transcripts from cDNAs encoding wild type tRNAs of E.coli and their synthesized mutants. These sera revealed different reactivity to mutated tRNAs. Eleven of these 13 patients met disease specific criteria for : SLE (3 patients), Sjogren's syndrome (6), or SLE/Sjogren's syndrome overlap (2). In addition, fever, Raynaud's phenomenon, and nonerosive polyarthritis, were frequently found in these patients. In summary, we have identified novelantibodies to tRNAs which appear to be quite common, and distinct from previously described anti-aminoacyl-tRNA synthetase antibodies.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (35 results)

All Other

All Publications (35 results)

  • [Publications] Ohosone Y.: "Clinical characteristics related to methotrexate-induced pancytopenia" Clin Rheumatol. 16. 321-323 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohosone Y.: "Clinical characteristics of Patients with Rheumatoid Arthritis and Methotrexate-induced Pneumonitis" J Rheumatol. 24. 2299-2305 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohosone Y.: "Anti-transfer RNA Antibodies in Two Patients with Pulmonary Fibrosis, Raynaud's Phenomenon, and polyarthritis." Clin Rheumatol. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohosone Y.: "Spectrum and clinical significance of autoantibodies against transfer RNAs" Arthritis Rheum. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 大曽根 康夫: "慢性関節リウマチにおけるメトトレキサート療法の副作用 ーとくに汎血球減少症と間質性肺炎例の背景因子に関する検討" リウマチ. 37. 1623-1627 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 松村 麻実: "リウマチ性疾患におけるトランスファーRNAに対する新しい自己抗体" リウマチ. 37. 563-556 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsumura M.: "Novel autoantibodies directed against the common tertiary configuration of transfer RNAs from patient with interstitial lung disease" Arthritis Rheum. 39(8). 1308-1312 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohosone Y.: "Spectrum and clinical significance of autoantibodies associated with transfer RNAs(tRNAs)" Arthritis Rheum. 39(9). S40 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 大曽根 康夫: "自己抗体とは何かー病因,病態,病像との関連はー" シンポジウム免疫と炎症. 5. 51-57 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 大曽根 康夫: "自己抗原遺伝子" Biotherapy. 10. 1515-1519 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 力石 昭宏: "間質性肺炎と壊死性半月体形成性糸球体腎炎を認め,ミエロベルオキシダーゼに対する抗好中球細胞質抗体陽性であった1例" リウマチ. 36(6). 869-873 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Konishi M.: "Mixed-cryoglobulinemia associated with cutaneous vasculitis and pulmonary symptoms" Internal Med.36(1). 62-67 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 大曽根 康夫: "内科学教科書(医学書院)" アナフィラキシ-(印刷中),

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsumura M,Ohosone Y,et al: "Novel autoantibodies directed against the common tertiary configuration of transfer RNAs from patient with interstitiallung disease" Arthritis Rheum. 39. 1308-1312 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohosone Y: "genes encoding autoantigen" Biotherapy. 10. 1515-1519 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Konishi M,Ohosone Y,et al: "Mixed-cryoglobulinemia associated with cutaneo us vasculitis and pulmonary symptoms" Intemal Med. 36. 62-67 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohosone Y et al: "Toxicity of low-dose Methotrexate in rheumat oidarthritis-Clinical characteristics in patients with MTX-induced pancytopenia and interstitial pneumonitis" Riumati. 37. 16-23 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohosone y, Okano Y,Kameda H,Hama N,Mimori T,Akizuki M,Ikeda Y: "Clinical characteristics related to methotrexate-induced pancytopenia" Clin Rheumatol. 16. 321-322 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Matsumura M,Ohosone Y,et al: "Novel autoantibodies against tRNA in patients with rheumatic diseases" Riumati. 37. 556-563 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohosone Y,et al: "Clinical Characteristics of Patients with Rheumatoid Arthritis and Methotrexate-induced Pneumonitis" J Rheumatol. 24. 2299-2305 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohosone Y,et al: "Anti-transfer RNA Antibodies in Two Patients with Pulmonary Fibrosis, Raynaud's Phenomenon, and Polyarthritis" Clin Rheumatol. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohosone Y,et al: "Spectrum and clinical significance of autoantibodies against transfer RNAs" Arthritis Rheum. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohsone Y: "Clinical characteristics related to methotrexate-induced pancytopenia" Clin Rheumatol. 16. 321-323 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ohosone Y: "Clinical Characteristics of Patients with Rheumatoid Arthritis and Methotrexate-induced Pneumonitis" J Rheumatol. 24. 2299-2305 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ohosone Y: "Anti-transfer RNA Antibodies in Two Patients with Pulmonary Fibrosis,Raynaud's Phenomenon,and Polyarthritis." Clin Rheumatol. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] Ohosone Y: "Spectrum and clinical significance of autoantibodies against transfer RNAs" Arthritis Rheum. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] 大曽根康夫: "慢性関節リウマチにおけるメトトレキサート療法の副作用-とくに汎血球減少症と間質性肺炎例の背景因子に関する検討" 'リウマチ'. 37. 1623-1627 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 松村麻実: "リウマチ性疾患におけるトランスファーRNAに対する新しい自己抗体" リウマチ. '37. 563-556 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 大曽根康夫: "内科学教科書(医学書院)" アナフィラキシ-(印刷中),

    • Related Report
      1997 Annual Research Report
  • [Publications] Matsumura M: "Novel aut oantib odies directed against the common tertiary configuration of transfer RNAs from patient with interstitial lung disease" Arthritis Rheum. 39(8). 1308-1312 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Ohosone Y: "Spectrum and clinical significance of aut oantib odies associated with transfer RNAs(tRNAs)" Arthritis Rheum. 39(9). S40 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 大曽根康夫: "自己抗体とは何か-病因,病態,病像との関連は-" シンポジウム免疫と炎症. 5. 51-57 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 大曽根康夫: "自己抗原遺伝子" Biotherapy. 10. 1515-1519 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 力石昭宏: "間質性肺炎と壊死性半月体形成性糸球体腎炎を認め,ミエロペルオキシダーゼに対する抗好中球細胞質抗体陽性であった1例" リウマチ. 36(6). 869-873 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Konishi M: "Mixed-cryoglobulinemia associated with cutaneous vasculitis and pulmonary symptoms" Internal Med. 36(1). 62-67 (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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