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Fas mediated apoptosis in sialoadenitis and interstitial nephritis associated with Sjogren's syndrome

Research Project

Project/Area Number 08670536
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionToho University

Principal Investigator

MATSUMURA Ryutaro  Toho University, School of Medicine, Assistant, 医学部, 助手 (70246742)

Co-Investigator(Kenkyū-buntansha) KAGAMI Masaru  Toho University, School of Medicine Assistant, 医学部, 助手 (50233656)
TOMIOKA Hisao  Toho University, School of Medicine, Professor, 医学部, 教授 (20009632)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
KeywordsSjogren's syndrome / sialoadenitis / intersfitial nephrotis / Fas antigen / Fas ligand / Apoptosis / aly mice
Research Abstract

Objective. To evaluate the role of Fas-Fas ligand system mediated apoptosis in the sialoadenitis and interstitial nephritis of sjogren's syndrome.
Methods. The expression of Fas antigen and Fas ligand in sialoadenitis and interstitial nephritis was examined by immunoperoxidase staining and reverse transcriptase-polymerase reaction (RT-PCR) in patients with Sjogren's syndrome and normal subjects. The appearance of DNA strand breaks during apoptosis was detected in the tissue by DNA nick end labeling methods.
Results. In patients with severe sialoadenitis, Fas antigen was strongly expressed on the ductal epithelial cells. By contrast, Fas antigen was not seen in the minor salivary glands of normal subjects and those having cases of mild sialoadenitis. In the patients with massive mononuclear cell infiltration, some infiltrating cells showed Fas ligand. In patients with interstitial nephritis associated with Sjogren's syndrome, Fas was expressed on tubular epithelial cells. On the other hand, Fas antigen was not observed on tubular epithelial cells of control subjects without interstitial nephritis. In the patients with interstitial nephritis, some infiltrating cells showed Fas ligand. Apoptotic change was observed in ductal epithelial cells, tubular epithelial cells and some infiltrating cells by DNA nick end labeling methods. The massage of Fas antigen and Fas ligand was found to be expressed in the labial salivary glands from all SS patients by RT-PCR.
Conclusion. This finding suggests that Fas-Fas ligand system may play a role in the pathogenesis of sialoadenitis and also of interstitial nephritis in Sjogren's syndrome.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Ryutaro Matsumura: "Expression of ductal Fas antigen in sialoadenitis of Sjogren's syndrome." Clin Exp Rheum. 14. 309-311 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 松村 竜太郎: "Sjogren症候群の尿細管間質性腎炎におけるFas-Fasリガンド系によるアポトーシス" 医学のあゆみ. 178. 791-796 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 松村 竜太郎: "シェ-グレン症候群の小唾液腺炎組織におけるFas抗原の発現" 日本臨床. 53. 2446-2450 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ryutaro Matsumura: "Expression of ductal Fas antigen in sialoadenitis of Sjogren's syndrome" Clin Exp Rheum. 14. 309-311 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ryutaro Matsumura: "Expression of Fas antigen and Fas ligand in tubulointerstitial nephritis associated with Sjogren's syndrome" Igaku no Ayumi. 178. 791-796 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ryutaro Matsumura: "Expression of ductal Fas antigen in sialoadenitis of Sjogren′s syndrome." Clin Exp Rheum. 14. 309-311 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 松村竜太郎: "Sjogren症候群の尿細管間質性腎炎におけるFas-Fasリガンド系によるアポトーシス" 医学のあゆみ. 178. 791-796 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 松村竜太郎: "シェ-グレン症候群の小唾液腺炎組織におけるFas抗原の発現" 日本臨床. 53(10). 2446-2450 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ryutaro Matsumura: "Expression of ductal Fas antigen in sialoadenitis of Sjogren's syndrome." Clin Exp Rheum. 14. 309-311 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 松村竜太郎: "Sjogren症候群の尿細管間質性腎炎におけるFas-Fasリガンド系によるアポトーシス" 医学のあゆみ. 178. 791-796 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 松村竜太郎: "シェ-グレン症候群の小唾液腺炎組織におけるFas抗原の発現" 日本臨床. 53(10). 2446-2450 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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