| Project/Area Number |
08670536
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | Toho University |
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Principal Investigator |
MATSUMURA Ryutaro Toho University, School of Medicine, Assistant, 医学部, 助手 (70246742)
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| Co-Investigator(Kenkyū-buntansha) |
KAGAMI Masaru Toho University, School of Medicine Assistant, 医学部, 助手 (50233656)
TOMIOKA Hisao Toho University, School of Medicine, Professor, 医学部, 教授 (20009632)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | Sjogren's syndrome / sialoadenitis / intersfitial nephrotis / Fas antigen / Fas ligand / Apoptosis / aly mice |
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| Research Abstract |
Objective. To evaluate the role of Fas-Fas ligand system mediated apoptosis in the sialoadenitis and interstitial nephritis of sjogren's syndrome.
Methods. The expression of Fas antigen and Fas ligand in sialoadenitis and interstitial nephritis was examined by immunoperoxidase staining and reverse transcriptase-polymerase reaction (RT-PCR) in patients with Sjogren's syndrome and normal subjects. The appearance of DNA strand breaks during apoptosis was detected in the tissue by DNA nick end labeling methods.
Results. In patients with severe sialoadenitis, Fas antigen was strongly expressed on the ductal epithelial cells. By contrast, Fas antigen was not seen in the minor salivary glands of normal subjects and those having cases of mild sialoadenitis. In the patients with massive mononuclear cell infiltration, some infiltrating cells showed Fas ligand. In patients with interstitial nephritis associated with Sjogren's syndrome, Fas was expressed on tubular epithelial cells. On the other hand, Fas antigen was not observed on tubular epithelial cells of control subjects without interstitial nephritis. In the patients with interstitial nephritis, some infiltrating cells showed Fas ligand. Apoptotic change was observed in ductal epithelial cells, tubular epithelial cells and some infiltrating cells by DNA nick end labeling methods. The massage of Fas antigen and Fas ligand was found to be expressed in the labial salivary glands from all SS patients by RT-PCR.
Conclusion. This finding suggests that Fas-Fas ligand system may play a role in the pathogenesis of sialoadenitis and also of interstitial nephritis in Sjogren's syndrome.
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