| Project/Area Number |
08670543
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | Kurume University |
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Principal Investigator |
SAGAWA Kimitaka Kurume University, School of Medicine, Professor, 医学部, 教授 (20140650)
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| Co-Investigator(Kenkyū-buntansha) |
MOCHIZUKI Manabu Kurume University, School of Medicine, Professor, 医学部, 教授 (10010464)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Behcet's disease / cloned T cells / T cell clones / cytokines / IL-8 / TNF-alpha / hydrocortisone / FK506 |
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| Research Abstract |
Behcet's disease is a systemic inflammatory disease immunologically characterized by the infiltration of CD4+T cells and polymorphonuclear cells in the affected tissues, including the eye. Despite extensive studies, neither the etiology nor the pathogenic mechanism of Behcet's disease has been clarified. Production of cytokines by T cell clones (TCC) from the ocular fluid (18 CD3+CD4+CD8- clones and 4 CD3+CD4-CD8+clones) and peripheral blood mononuclear cells (PBMC) (16 CD3+CD4+CD8- clones) of two patients with uveitis associated with Behcet's disease was investigated to understand better the immunopathology of the disease. The level of IL-8 spontaneously produced by TCC from the ocular fluid (mean : 659 pg/ml) or PBMC (536 pg/ml) of the patients was significantly higher (p<0.005, and p<0.05, respectively) than that by TCC from PBMC of healthy donors (241 pg/ml). As for the other cytokines, IL-1alpha, IL-2, IL-6, IL-10, and TNF-alpha were produced at low but detectable amounts by the majority of TCC from the ocular fluid of patients with Behcet's disease ; IL-3, IFN-gamma and GM-CSF were also produced by some of TCC at low amounts ; and IL-4 was not produced by any TCC tested. IL-8 production by TCC from the ocular fluid was further up-regulated upon stimulation with PHA,but was suppressed by FK506 and hydrocortisone, though not by diclofenac sodium. Colchicine rather increased IL-8 production by these TCC.These results suggest that T cells in both the affected tissues and PBMC play an important role in the pathogenesis of uveitis associated with Behcet's disease through IL-8 and probably the other cytokines.
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