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Study on two new microglia growth factors purified from cloned culture cells

Research Project

Project/Area Number 08670545
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionNational Center of Nerology and Psychiatry (NCNP)

Principal Investigator

KIKUCHI Aiko  National Institute of Neuroscience, Department of Ultrastructural Research, Researcher, 神経研究所・微細構造研究部, 研究員 (70159010)

Co-Investigator(Kenkyū-buntansha) KAMO Isao  National Institute of Neuroscience, Department of Ultrastructural Research, Sect, 神経センター・神経研究所・微細構造研究部, 室長 (70108489)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsmicroglia / monocyte / macrophage / growth factor / M-CSF / brain / thymus / myoid cell / GM-CSF / 培養細胞 / 分化増殖因子 / アストログリア
Research Abstract

We have previously found that an 80 kDa factor and a 100 kDa factor in the conditioned medium of cloned thymic myoid cells. Amino acid analysis and biological study of these factors indicate that they are different from the authentic GM-CSF and M-CSF and also different from each other. These two factors stimulate growth and differentiation of monocytic lineage cells from various organs, including the brain microglial cells. Generally, since the brain and the thymus share many biological characteristics, such as cell surface antigens and cytokines, we attempted to locate possible producer cells in the brain by using cDNA probes and antibodies specific to these two factors. We have found that the brain indeed produce two mosecules identical to the two factors derived from the thymic nyoid cels, together with other cytokines, such as M-CSF.These results suggest that development of monocytic lineage cells depend on plural growth and differentiation factors, probably a certain combination of monocytic cell growth facters are important for generation of the heterogeneity in monocytic lineage cells. We are in progress to produce their recombinant factors in large scale and to prepare the knockout mice of these factors to establish their biological significance.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report

Research Products

(24 results)

All Other

All Publications

  • [Publications] N.Iwakami, A.Kikuchi, T.Kunishita, I.Nonaka, I.Kamo: "Analysis of lymphoproliferative cytokines produced by thymic myoid cells" Immunology. 87. 108-112

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Sakuragawa, I.Kamo, , et al: "Expression of markers for both neuronal and glial cells in human ammnitotic epithelial cells" Neuroscience Letter. 209. 2-12 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] I.Kamo, A.Kikuchi: "Monocytic lineage cell growth and differentiation factors produced by thymic myoid cells" European Journal of Heamatology. 57. 31-31 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Kikuchi, I.Kamo: "Thymic myoid cells produce cytokines for lymphocytes and monocytic cells" Cancer Detection and Prevention. 20. 395-395 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Kikuchi et al: "Haemopoietic biglycan produced by brain cells stimulates growth of microglial cells" Joumal of Neuroimmunology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Tomoyasu et al.: "Identificauon of haemopoietic biglycan in hyperplastic thymus asoctated with myasthnia gravis" Journal of Neuroimmunology. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Kikuchi, H.Tomoyasu, I.Kamo: "J.Neuroimmunology(in Japanese)" The production of Haemopoieticc Biglycan from Brain Cells, 58-59 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Iwakami, A.Kikuchi, T.Kunishita, H.Yamamoto, I.Nonaka, I.Kamo: "Analysis of lylmphoproliferative cytokines produced by thymic myoid cells" Immunology. 87. 108-112 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Sakuragawa, I.Kamo: "Expression of markers for both neuronal and glial cells in human ammnitotic epithelial cells" Neurosci.Lett.209. 2-12 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] I.Kamo, A.Kikuchi: "Monocytic lineage cell growth and differentiation factors produced by thymic myoid cells" Eur.J.Haematl. 57. 31-31 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Kikuchi, I.Kamo: "Thymic myoid cels produce cytokines for lymphocytes and monocytic cells" Cancer Detect and Prevent. 20. 395-395 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Kikuchi et al.: "Haemopoietic biglycan produced by brain cells stimulates growth of microglial cells" J.Neuroimmunol. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] H.Tomoyasu et al.: "Identification of haemopoietic biglycan in hyperplastic thymus associated with myasthenia gravis" J.Neuroimmunol. (in press). (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Kikuchi, H.Tomoyasu, I.Kamo: "The production of Haemopoietic Biglycan from Brain Cells (in Japanese)" Neuroimmunology. 6. 58-59 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] N.Iwakami,A.Kikuchi,T.Kunishita,I.Nonaka,I.Kamo: "Analysis of lymphoproliferative cytokines produced by thymic myoid cells" Immunology. 87. 108-112

    • Related Report
      1997 Annual Research Report
  • [Publications] N,Sakuragawa,I.Kamo,,et al.: "Expression of markers for both neuronal and glial cells in human ammniotic epithelial cells" Neuroscience Letter. 209. 2-12 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] I.Kamo,A.Kikuchi: "Monocytic lineage cell growth differentiation factors produced by thymic myoid cells" European Journal of Heamatology. 57. 31-31 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] A.Kikuchi,I.Kamo: "Thymic myoid cells produce cytokines for lymphocytes and monocytic cells" Cancer Detection and Prevention. 20. 395-395 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] A.Kikuchi et al.: "Haemopoietic biglycan produced by brain cells stimulates growth of microglial cells" Journal of Neuroimmunology. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] H.Tomoyasu et al.: "Identification of haemopoietic biglycan in hyperplastic thymus asocitated with myasthenia gravis" Journal of Neuroimmunology. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] A.Kikuchi,H.Tomoyasu,I.Kamo: "J. Neuroimmunology(in Japanese)" The production of Haemopoietic Biglycan from Brain Cells, 58-59 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] I.Kamo: "Expression of markers for both neuronal and glial cells in human ammniotic epithelial cells" Neuroscience Letters. 209. 9-12 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] I.Kamo,A.Kikuchi: "Monocytic lineage cell growth and differentiation factors produced by thymic myoid cells" Eur.J.Haematology. 57(supl). 31-31 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] A.Kikuchi,I.Kamo: "Thymic myoid cells produce multiple cytokines for lymphocytes and monocytic cells" Cancer Detection and Prevention. 20. 395-395 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-03-31   Modified: 2016-04-21  

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