| Project/Area Number |
08670546
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | HIROSAKI UNIVERSITY |
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Principal Investigator |
MUNAKATA Akihiro HIROSAKI UNIVERSITY,SCHOOL OF MEDICINE,PROFESSOR, 医学部, 教授 (50003661)
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| Co-Investigator(Kenkyū-buntansha) |
UNO Yoshiharu HIROSAKI UNIVERSITY,SCHOOL OF MEDICINE,Instructor, 医学部附属病院, 助手 (50271812)
FUKUDA Shinsaku HIROSAKI UNIVERSITY,SCHOOL OF MEDICINE,Instructor, 医学部, 助手 (60261450)
NAKAJI Shigeyuki HIROSAKI UNIVERSITY,SCHOOL OF MEDICINE,ASSISTANT PROFESSOR, 医学部, 講師 (10192220)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1996: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | resistant starch / dietary fiber / cellulose / 1,2-dimethylhydrazine / colorectal cancer / short-chain fatty acid / butyrate / stool / 1.2-demethylhydrazine |
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| Research Abstract |
Epidemiological and experimental studies have shown that dietary fiber may prevent colon cancer. Resistant starch, like kietary fiber, is not subject to digestion in the small intestine. However, it is unknown whether resistant starch inhibits colonic carcinogenesis. In vitro studies have shown that butyrate shows the growth of cultured colon cancer cells. The effect of resistant starch diet on 1,2-dimethy l hydrazine-induced colonic carcinogenesis in rats was evaluated, and the colonic butyrate concentration was measured. Sprague-Dawlet rats were randomly divided into five groups that were fed diets containing no fiber, 30% cellulose, 10% cellulose, 3% resistant starch, or 10% resistant starch. Colonic carcinogenesis and butyrate concentration of colonic contents and feces in each diet group were compared.
Total cancer volume per rat in the 10% cellulose group was significantly lower than that in the basal group (109 54 mm^3 and 147 83mm^3 ; p<0.05), but the other groups showed no significant differences. The butyrate concentration in colonic content and in feces were higher in the resistant starch group than in the cellulose group. The resistant starch siet increased butyrate concentration but did not inhib it colonic carcinogenesis. It remains doubtful wether butyrate inhibits the proliferation of colon cancer cells.
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