Role of Nitric Oxide in Regulation of Gastric Motility, Gastric Emptying and Pancreato-Biliary Secretion.
| Project/Area Number |
08670556
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Gunma University |
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Principal Investigator |
MIZUMOTO Akiyoshi Institute for Molecular and Cellular Regulation, Gunma University, Research Assistant, 生体調節研究所, 助手 (00272261)
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| Co-Investigator(Kenkyū-buntansha) |
ITOH Zen Institute for Molecular and Cellular Regulation, Gunma University, Professor, 生体調節研究所, 教授 (00008294)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | gastrointestinal motility / gastric emptying / pancreato-biliary secretion / nitric oxide / interdigestive contractions / NO synthase inhibitor / conscious dog / motilin / Nitric Oxide |
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| Research Abstract |
In the present research, we investigated the role of endogenous nitric oxide (NO) in regulation of gastric motility, gastric emptying and pancreato-biliary secretion in conscious dogs.
l) Interdigestive motility and motilin release
The effect of inhibition of NO synthase on interdigestive gastric motility and motilin release was examined in dogs fitted with force transducers in the gastric antrum. N^G-nitro-L-arginine (L-NNA) at a dose of 3 mg/kg-h was given intravenously for 1 hour during a quiescent period. Blood samples were taken at 5 - 10 min intervals for measurement of plasma motilin concentrations. As a result, L-NNA induced premature interdigestive migrating contractions in the stomach and also increased plasma motilin concentrations
2) Postprandial gastric motor activities and emptying
The effect of L-NNA on postprandial gastric motility and gastric emptying was examined. Dogs with force transducer in the gastric antrum and two duodenal catheters were used in the study. Phenolsulfonphtalein was continuously infused into the proximal duodenum to determine the duodenal volume. Polyethylene glycol was used as a liquid marker and solid gastric emptying was determined by a freeze-drying method developed by us. Gastric contractions were assessed by computer analysis. As a result, L-NNA dose dependently increased postprandial gastric contractions, while it significantly inhibited gastric emptying of liquid and solids.
3) Postprandial pancreato-biliary secretion
Same model as mentioned above was used in the study. The effect of L-NNA on postprandial pancreato-biliary secretion was examined. The concentrations ofamylase and bile acid were determined by spectrophotometer. L-NNA markedly inhibited postprandial amylase secretion and tended to inhibit biliary secretion.
These findings suggest that endogenous NO plays an important role in regulation of interdigestive and postprandial gastric contractions, gastric emptying and pancreato-biliary secretion.
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Report
(3 results)
Research Products
(21 results)
