| Project/Area Number |
08670610
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Gastroenterology
|
|---|
| Research Institution | Osaka City University, Medical School |
|---|
Principal Investigator |
KUROKI Tesuo Osaka City University, 3rd.Department of Internal Medicine, Professor, 医学部, 教授 (30047328)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NISHIGUCHI Shuhei Osaka City University, 3rd.Department of Internal Medicine, Assistant Professor, 医学部, 講師 (10192246)
SEKI Shuichi Osaka City University, 3rd.Department of Internal Medicine, Associate Professor, 医学部, 助教授 (50145778)
|
|---|
| Project Period (FY) |
1996 – 1998
|
| Project Status |
Completed (Fiscal Year 1998)
|
| Budget Amount *help |
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
|
|---|
| Keywords | hepatocarcinogenesis / interferon / basic FGF / prevention / anti-proliferative effects / metastasis / IFN / 肝癌 / bFGF / 肝化学発癌モデル / 肝癌発症予防 / promotion / progression / GST-p / poly I : C |
|---|
| Research Abstract |
To elucidate how the down-regulation of basic fibroblast growth factor (bFGF) in hepatocellular carcinoma by interferon (IFN) - alpha and -beta is related to the antiproliferative effects of lFN- alpha and-beta and to determine whether bFGF and IFNs can alter the invasiveness of hepatocellular carcinoma, I examined four hepatoma cell lines (PLC/PRF/5, HLF, HepG2, Huh-7). Anti-proliferative effects of IFN-alpha and-bete were strongest for PLC/PRF/5, relatively weak for Huh-7, and not observed for HLF and HepG2. IFN- gamma did not effect on all cell lines. The expression of bFGF was intense in HLF, PLC/PRF/5, Huh-7, and HepG2 in that order. The down-regulation of bFGF by IFN- alpha and -beta was observed only for PLC/PRF/5, and bFGF affected cell proliferation in hepatoma cell line cultures. These findings suggest that down-regulation of bFGF by lFN-alpha and -beta may be one of the mechanisms responsible for the anti-proliferative effects of lFN-alpha and -beta. In my in vitro invasion system, IFNs inhibited the invasiveness of hepatoma cell lines slightly, and bFGF did not increase their invasiveness.
Conclusion : (1) IFN - alpha and -beta inhibited the proliferation of hepatoma cell lines partly by down-regulation of bFGF. ; (2) IFNs decreased the invasiveness of hepatoma cell lines slightiy. ; and (3) bFGF had no effect on the invasiveness of hepatoma cell lines.
|
