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The gastrin gene expression and transcriptional regulation

Research Project

Project/Area Number 08670614
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionWakayama Medical College

Principal Investigator

SHIOTANI Akiko  Wakayama Medical College, second Dept.of Internal Medicine, Assistant professor, 医学部, 講師 (80275354)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsGastrin gene / Sp1 / EGF / Okadaic acid / PGE_2 / 転写制御 / gERE / Sp1 / Okadaic acid / bFGF
Research Abstract

Sp1 nuclear levels have been shown to directly correlate with the proliferative state of the cells. It is known that EGF receptor is activated by a series of reaction of phosphorylation, and post-transcriptional modification of Sp1 by phosphorylation and glycosylation has been correlated with Sp1 transcriptional activation in vitro. We therefore studied changes in the Sp1 nuclear levels in a rat pituitary cell line GH4 stimulated by EGF and Okadaic acid which is a phosphatase inhibitor. Using nuclear extracts from GH4 cells treated with l0nM EGF foe at least 16h, Sp1 binding decreased without a significant changes in the gERE1 and 2 complexes. Immunoblot analysis using the nuclear extracts treated with EGF showed that there was a decrease in the nuclear levels of Sp1. Pretreatment with EGF had little effect on the basal activity of the gastrin promoter. However, the the basal activity was decrease in basal activation of mutated gERE and Sp1 element which confer higher basal activity than the gERE.Treatment of the cells with okadaic acid alone resulted in a similar or greater decrease in Sp1 nuclear levels compared with EGF alone. Treatment of the cells with both EGF and okadaic acid had a synergistic effect, further depressing nuclear levels of Sp1. The loss of Sp1 was observed with EGF and augmented by okadaic acid, suggesting that activation of the EGF receptor phosphorylates Sp1 through increased intracellular serine/threonine protein kinase activity.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] 塩谷昭子: "ヒトガストリン遺伝子におけるCAMPによる発現制御に関する研究" 和歌山医学. 46. 335-344 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Juanim L Monhant: "Epicernal growth factor simulation of the human gastrin promotor requires SPI." The Journal of Biological Chemistry. 270. 6314-6319 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akiko Shiotani: "CAMP regulates gastrin gene expression" American Journal Physiology. 269. G458-464 (1995)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 塩谷昭子: "ガストリン遺伝子の発現調節および転写制御" 医学のあゆみ. 176. 802-805 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] 塩谷昭子: "ヒトガストリン遺伝子におけるEGFによる転写制御" 日本臨床. 54. 1087-1091 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mortensem RE: "Epicermal growth factor and Okadaic acid Simulare Spl Proteolysis" The Journal of Biological Chemistry. 272. 16540-16547 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Juanita L Merchant, Akiko Shiotani, Eric R Mortensen, Dale K Shumaker and Diane R Abraczinskas: "Epidermal growth factor stimulation of the human gastrin promoter requires Sp1" The Journal of Biological Chemistry. 270. 6314-6319 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Akiko Shiotani and Juanita L Merchant: "cAMP regulates gastrin gene expression" American Journal Physiology. 269. G458-464 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Mortensen RE, Marks AP, Shiotani A and Merchant JL: "Epidermal Growth Factor and Okadaic Acid Stimulate Sp1 Proteolysis" The Journal of Biological Chemistry. 272. 16540-16547 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Taniuchi T, Mortensen ER, Ferguston A, Geenson J, Merchant JL: "Overexpression of ZBP-89, a zinc finger DNA binding protein, in gastric cancer" Biochem Biophys Res commum. 233(1). 154-160 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Cicala C, Santacrose C, Itoh H, Douglas GJ, Page CP: "A study on rat platelet responsiveness following intravenous endotoxin administration" Life Sci. 60(2). 31-38 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A Shiotani, K Yanaoka, M Iguchi, A Saika, H Itoh, S Nishioka: "Helicobacter pylori infection decreases nitric oxide in the stomach" Gut. 43(suppl 2). A23 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K Arii, and JL Merchant: "Correlation of Sp1 and ZBP-89 protein expression with gastric cancer subtype" Gastroenterology. 114. A558 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] A.Shiotani, K Yanaoka, M Iguchi, T Ishiguchi, T Hara, H itoh, S Nishioka: "Increased nitric oxide after eradication of Helicobacter pylori" Gastroenterology. 114. A1084 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] J.L Merchant, A.Shiotani et al: "Epidermal Growth Factor Stimulation of the Human Gastrin Promoter Riquires Sp1" The Journal of Biological Chemistry. 270. 6314-6319 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] A.Shiotani,J.L Merchant: "cAMP regulates gastrin gene expression" The American Journal of Physiology. 269. G458-G464 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] 塩谷昭子: "ヒトガストリン遺伝子におけるcAMPによる発現制御に関する研究" 和歌山医学. 46. 335-344 (1995)

    • Related Report
      1997 Annual Research Report
  • [Publications] 塩谷昭子: "ガストリン遺伝子の発現調節および転写制御" 医学のあゆみ. 176. 802-805 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] 塩谷昭子: "ヒトgastrin遺伝子におけるEGFによる転写制御" 日本臨床. 54. 1087-1091 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] E.R.Mortensen, A.Shiotani et al: "Epidermal Growth Factor and Okadaic Acid Stimulate Sp1 Proteclysis" The Journal of Biological Chemistry. 272・26. 16540-16547 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] J.L.Merchent, A Shiotani et al: "Epidermol Growth Factor Stimulation of the Human Gnstrin Promoter Riquires Sp1" The Journal of Biological Chemistry. 270. 6314-6319 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] A Shiotani, J.L.Merchent: "cAMP regulates gastrin gene expression" The American Journal of Physiology. 269. G458-G464 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 塩谷昭子: "ヒトガストリン遺伝子におけるcAMPによる発現制限に関する研究" 和歌山医学. 46. 335-344 (1995)

    • Related Report
      1996 Annual Research Report
  • [Publications] 塩谷昭子: "ガストリン遺伝子の発現調節および転写制御" 医学のあゆみ. 176. 802-805 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 塩谷昭子: "ヒトgastrin遺伝子におけるEGFによる転写制御" 日本臨床. 54. 1087-1091 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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