|Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1997: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
1.We performed pre-endoscopic serological screening of H.pylori infection in 386 Japanese patients. Among 289 H.pylori-seropositive patients, 60 gastric ulcers, 59 duodenal ulcers, and 1 gastric cencer were found. By contrast, only three peptic ulcer patients were found among 97 H.pylori-seronegative patients. Interestingly, among 25 patients with fundic gland polyp (s), 24 were H.pylori-seronegative, suggsting that these patients are resistant to H.pylori infection.
2.Diversity of vacuolating cytotoxin gene (vacA gene) of H.pylori, one of the most important pathogenetic factor of H.pylori was examined by long-PCR based RFLP analysis. Entire coding region of vacA gene (about 3.9kBp) was amplified by long-PCR,and digested with restriction enzymes. We found multiple RFLP patterns, indicating significant diversity of vacA gene among H.plyori strains isolated in Japan. 3.Chemokine expression was examined in H.plyori-infected gastric mucosa. In addition to CXC-chemokine IL-8, expression of CC-chemokines, such as MCP-1 and RANTES,was identified in H.pylori-infected gastric mucosa. We also found that gastric epithelial cells express CC-chemokines in response to proinflammatory cytokines and H.pylori infection in vitro.