| Project/Area Number |
08670627
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | TOKAI UNIVERSITY |
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Principal Investigator |
KAGAWA Tatehiro Tokai University School of Medicine Assistant Professor, 医学部, 講師 (30245469)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIZAKI Yasuhiro Tokai University School of Medicine Assistant Professor, 医学部, 講師 (80237693)
WATANABE Norihito Tokai University School of Medicine Associate Professor, 医学部, 助教授 (90167156)
岡崎 有博 東海大学, 医学部, 助手 (40266391)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | antisense / K-ras / pancreatic cancer / gene therapy |
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| Research Abstract |
1. Establishment and characterization of a pancreatic cancer cell line
A pancreatic cancer cell line, Pca-S was established from a 60 year-old woman bearing pancreatic carcinoma. The DNA analysis revealed a mutation from GGT (Gly) to GTT (Val) in K-ras codon 12.
2. Phosphorothioate oligodeoxynucleotides
Antisense phosphorothioate oligodeoxynucleotides (AS) were designed against codon 12 of K-ras mRNA.Sense phosphorothioate oligodeoxynucleotides against codon 12 of v-K-ras mRNA were used as the controls.
3. Effect of AS on proliferation of Pca-S cell line
Cells were cultured in RPMl-1640 containing 10% fetal calf serum. AS were added on days 0 and 1 at various concentrations, and the proliferation of cells was evaluated using MTT on days 4 and 7. AS with concentrations of 5 and 10 muM specifically inhibited the proliferation up to 35% on day 4. However, on day 7, the anti-proliferative effects of AS were abolished, indicating that the effect of AS was not lasting. In summary, antisense phosphorothioate oligodeoxynucleotides against v-K-ras codon 12 would have anti-proliferative effect on pancreatic cancer cells with limited duration.
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