Down regulation of TGF-beta receptors in Human colorectal Canter
| Project/Area Number |
08670634
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Kansai Medical University |
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Principal Investigator |
SAWAMURA Takaya The third department of internal medicine, Kansai Medical University associate professor, 医学部, 助教授 (10105786)
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| Co-Investigator(Kenkyū-buntansha) |
TANI Kazuhiro The third department of internal medicine, Kansai Medical University instructor, 医学部, 助手 (20257934)
MATSUZAKI Koichi The third department of internal medicine, Kansai Medical University associate p, 医学部, 講師 (70278638)
WATANABE Toshihiko The third department of internal medicine, Kansai Medical University associate p, 医学部, 講師 (20201212)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1996: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | TGF-beta / TGF-beta receptor / colon cancer / colon adenoma |
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| Research Abstract |
Many colorectal canoer cells are resistant to the anti-proliferative effects of transforming growth factor-beta (TGF-beta). TGF-beta also acts as paracrine factor from cancer cells on their mesenchymal cells. The aim of this study was to examine the expression of TGF-beta and its receptors in human colorectal cancer tissue and determined any relationship with cancer growth. In situ hybridization detection of TGF-beta_1 type I and type II receptor mRNA and immunohistochemical staining of TGF-beta_1 were performed using 10 human colorectal adenomas, 21 colorectal advanced cancers and 10 normal colorectal mucosas as control. TGF-beta receptor mRNAs were expressed mainly by normal colorectal epithelial cells and adenoma. However, mRNAs for TGF-beta receptors were only faintly, if at all, expressed in 7 of 21 human advanced cancers. In addition, intense signals of TGF-beta_1 mRNA and the protein were detected in all colorectal cancers. TGF-beta receptor mRNAs and TGF-beta_1 protein were also distributed in fibroblasts and endothelial cells in the interstitum. The escape of human colon cancer from TGF-beta-mediated growth inhibition by transcriptional down-regulation of TGF-beta receptors as well as the effects of TGF-beta on stroma formation and angiogenesis indicate a possible role for TGF-beta in the progression of colon cancer in an intact host.
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Report
(3 results)
Research Products
(6 results)
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[Publications] Data M., Matsuzaki K., Matsushita M., Sakitani K., Okajima A., Shibano K., Yamamoto C., Ogata N., Okumura T., Seki T., Kubota Y., Kan M., McKeehan W.L., Inoue K.: "Differential expression of Transforming Growth Factor-beta receptors in hepatocytes and nonparenchymal cells of rat liver injury." Journal of Hepatology. (In press).
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