| Project/Area Number |
08670667
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Nagasaki University |
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Principal Investigator |
KOHNO Shigeru Nagasaki University School of Medicine Professor, 医学部, 教授 (80136647)
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| Co-Investigator(Kenkyū-buntansha) |
UDONO Heiichiro Nagasaki University School of Medicine Assistant Professor, 医学部, 助教授 (50260659)
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| Project Period (FY) |
1996 – 1998
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| Project Status |
Completed (Fiscal Year 1998)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1998: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Anti-C.neoformans / HSP 70 antibody / Cryptococcus neoformans / Pulmonary cryptococcosis / ATPase / Capsular antigen / HSP / cryptococcus neo-Reemans / cryptococcosis / HSP 70 / ストレス蛋白(HSP) / ワクチン / Th-1,Th-2反応 / ストレス蛋白質(HSP) |
|---|
| Research Abstract |
Cryptococcus neoformans causes infection in individuals with defective T-cell function, such as AIDS, as well as without underlying disease. It has been suggested that humoral as well as cellular immunity might play an important role in the immune response to C.neoformans infection. We have recently shown, using immunoblotting, that the 70-kDa heat shock protein (HSP) family of C.neoformans was the major target molecule of the humoral response in murine pulmonary cryptococcosis. In this study, we also used immunoblotting to define the antibody responses in the sera of 24 patients with pulmonary cryptococcosis - 21 proven and 3 suspected diagnoses. Anti-C.neoforinans HSP 70 antibody was detected in 16 of 24 (66.7%) patients with pulmonary cryptococcosis. Fourteen of 17 (82.3%) patients with high antigen titers (*1 : 8) and 2 of 7 (28.6%) patients with low titers (*1 : 4), had detectable levels of anti-HSP 70 antibody. Sera from patients positive for anti-HSP 70 antibody showed high titers in the Eiken latex agglutination test for the detection of serum cryptococcal antigen. Our results indicate that the 70-kDa heat shock protein family from C.neoforinans appears to be a major target molecule of the humoral response, not only in murine pulmonary cryptococcosis, but also in human patients with pulmonary cryptococcosis.
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