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Identification of pulmonary sarcoidosis related antigen.

Research Project

Project/Area Number 08670688
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionKurume University School of Medicine

Principal Investigator

OIZUMI Kotaro  Kurume Univ., Sch.Med., Dep.Medicine, Professor, 医学部, 教授 (70006097)

Co-Investigator(Kenkyū-buntansha) HOSHINO Tomoaki  Kurume Univ., Sch.Med., Dep.Immunol., Instructor, 医学部, 助手 (00261066)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1997: ¥700,000 (Direct Cost: ¥700,000)
KeywordsPulmonary sarcoidosis / Antigen / T lymphocytes / BALF / T cell receptor / サルコイドーシス症 / サルコイドーシス / TCR Vα / TCR Vβ / non-restpiction / HLA Class II / HLA Class I
Research Abstract

Sarcoidosis is a systemic granulomatous disease of unknown etiology characterized by the pronounced accumulation of CD4+T cell and macrophages in the affected organs. TCR variable (V) alpha and Vbeta gene usage in patients with sarcoidosis is still a matter of discussion. In this investigation, we analyzed TCR-Valpha and-Vbeta gene usage in bronchoalveolar lavage fluid (BALF) and peripheral blood mononuclear cells (PBMC) of 30 patients with active pulmonary sarcoidosis using an adapter ligation method, reverse transcriptase-polymerase chain reaction (RT-PCR), and sequence-specific oligonucleotide probe (SSOP) analyzes. There was no significant difference in TCR-Valpha or-Vbeta gene usage between BALF (n=12) or PBMC (n=27) of patients and PBMC of healthy subjects (n=10). Neither selective TCR-Valpha nor-Vbeta expansion was observed in the paired BALF and PBMC from seven of nine patients. However, selective expansions were observed in a few TCR-Valpha or-Vbeta subsets in the BALF or PBMC of some individuals. Although a modest increase in a few TCR-Valpha or-Vbeta subsets was observed in the BALF or PBMC of some individuals, the increased TCR-Valpha or-Vbeta subsets were not closely associated with the HLA-DRB1, DQA1, DQB1, and DPB1 alleles of these patients. These results suggest that TCR-Valpha or-Vbeta gene usage is not restricted in both lung and peripheral blood in the majority of patients with active pulmonary sarcoidosis.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Hoshino, T., et al.: "TCRγδ+ T cells in peripheral blood of patients with adult Still's disease." J.Rheumatol.23. 124-129 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shichijo, S., et al.: "Detection of MAGE-4 protein in sera of lung cancer patients." Jpn.J.Cancer Res.88. 414-419 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hoshino, T., et al.: "HLA-class I-resticted and tumor-specific CTL in tumor-infiltrating lymphocytes of patients with gastric cancer." Int.J.Cancer.70. 631-638 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Seki, N., et al.: "HLA-A locus-restricted and tumor-specific CTL in tumor infiltrating lymphocytes of patients with non-small cell lung cancer." Cell.Immunol.175. 101-110 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hoshino, T., et al.: "Non-restricted T cell receptor(TCR)-Vα and-Vβ gene usage in patients with pulmonary sarcoidosis." Clin.Exp.Immunol.108. 529-538 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohba, H., et al.: "Spectroscopic analysis of the cytoagglutinating activity of abrin-b isolated from Abrus precatorius seeds against leukemic cells." Biosci.Biotechnol.Biochem.61. 737-739 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hoshino, T., Ohta, A., Nakao, M., Ota, T., Inokuchi, T., Matsueda, S., Gouhara, R., Yamada, A., Itoh, K., Oizumi, K: "Increase of TCRgammadelta+ T cells in peripheral blood of three patients with adults still's deisease" J.Rheumatology. 23. 124-129 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shjchifo, S., Hoshino, T., Koufuji, K., Hayashi, A., Kawamoto, M., Kikuchi, M., Higuchi, T., Ichiki, M., Oizumi, K., Itoh, K.: "Detection of MAGE-4 protein in sera of lung cancer patients" Jpn. J.Cancer Res. 88. 414-419 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hoshino, T., Seki, N., Kikuchi, M., Kuramoto, T., Iwamoto, O., Kodama, I., Koufuji K., Takeda, J., Itoh, K.: "HLA-class I-resticted and tumor-specific CTL in tumor-infiltrating lymphocystes of patients with gastric cancer" Int. J.Cancer. 70. 631-638 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Seki, N., Hoshino, T., Kikuchi, M., Hayashi, A., Itoh, K.: "HLA-A locusrestricted and tumor-specific CTL in tumor infiltrating lymphocytes of patients with non-small cell lung cancer" Cell. Immunol. 175. 101-110 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hoshino, T., Suzuki, R., Tsuruta, Y., Matsutani, T., Kikuchi, M., Kawamoto, M., Gouhare, R., Shiraishi, T., Mochizuki, M., Itoh, K., Oizumi, K.: "Non-restricted T cell receptor (TCR) -Valpha and -Vbeta gene usage in patients with pulmonary sarcoidosis." Clin.Exp. Immunol.108. 529-538 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ohba, H., Yoyokawa, T., Yasuda, S., Hoshino, T., Itoh, K., Yamasaki, N.: "Spectroscopic analysis of the cytoagglutinating activity of abrin-b isolated from Abrus precatorius seeds against leukimic cells." Biosci. Biotechnol. Biochem.61. 737-739 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hoshino,T.,et al.: "TCRγδ+ T cells in peripheral blood of patients with adult Still's disease." J.Rheumatol.23. 124-129 (1996)

    • Related Report
      1997 Annual Research Report
  • [Publications] Shichijo,S.,et al.: "Detection of MAGE-4 protein in sera of lung cancer patients." Jpn.J.Cancer Res.88. 414-419 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hoshino,T.,et al.: "HLA-class I-resticted and tumor-specific CTL in tumor-infiltrating lymphocytes of patients with gastric cancer." Int.J.Cancer.70. 631-638 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Seki,N.,et al.: "HLA-A locus-restricted and tumor-specific CTL in tumor infiltrating lymphocytes of patients with non-small cell lung cancer." Cell.Immunol.175. 101-110 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hoshino,T.,et al.: "Non-restricted T cell receptor(TCR)-Vα and -Vβ gene usage in patients with pulmonary sarcoidosis." Clin.Exp.Immunol.108. 529-538 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ohba,H.,et al.: "Spectroscopic analysis of the cytoagglutinating activity of abrin-b isolated from Abrus precatorius seeds against leukemic cells." Biosci.Biotechnol.Biochem.61. 737-739 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hoshino,T.et al: "HLA-classI-restricted and tumor-specific,CTL in TIL of patients with gastric cancer" Int.J.Cancer. (in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hoshino,T.et al: "Non-restricted T cell receptor-Vα and Vβ gene usage in patients with sarcoidosis" Clin Exp.Immunol.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Seki,N,et al: "HLA-Alocus-restricted and tumor-specific CTLs in TIL of patients with lung cancer" Cell.Immunol.(in press). (1997)

    • Related Report
      1996 Annual Research Report
  • [Publications] Yamada,A.,et al.: "Immunohistochemically,defined CFA-1 on adenocarcinoma cell line is a distinct…" Cell.Immunol.166. 254-260 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hoshino,T.et al.: "TcRYd^+ Tcells in penpheral blood of patients with adult stills disease" J.Rhenmatol.23. 124-129 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Itoh,K et al.: "Petectation of MAGE・4 protein on Lung cancer" Int.J.Cancer. 64. 158-165 (1995)

    • Related Report
      1996 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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