• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Experimental analysis of therapeutic windows in cerebral ischemia

Research Project

Project/Area Number 08670725
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurology
Research InstitutionKeio University

Principal Investigator

TANAKA Kortaro  Keio University, Department of Neurology, Associate Professor, 医学部, 専任講師 (90129528)

Co-Investigator(Kenkyū-buntansha) NAGATA Eiichiro  Keio University, Department of Neurology, Instructor, 医学部, 助手 (00255457)
近藤 太郎  慶應義塾大学, 医学部, 助手 (40234942)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1998: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
KeywordsCerebral ischemia / Cerebral infarction / Therapeutic window / cyclic AMP / Signal transduction / Protein kinase / Penumbra
Research Abstract

Phosphorylation of cyclic AMP response element binding protein (CREB) is one of the most important mechanisms controlling various gene transcriptions. In the present study, the phosphorylation of CREB was examined immunohistochemically at 24 hours of recirculation following 1.5 hours of middle cerebral artery occlusion (MCAO) in rats. MCAO was induced by the intraluminal suture method. The infarct core revealed a significant reduction in the number of immunoreactive cells with the anti-phosphorylated CREB and with the anti-CREB antibody, which binds to both unphosphorylated and phosphorylated CREB.In contrast, the pen-infarct area exhibited a marked increase in the number of immunopositive cells as well as in the intensity of nuclear staining with each antibody, so that almost all of the cells expressing CREB demonstrated phosphorylation of CREB.On the other hand, about half of the CREB immunopositive cells reacted weakly with the anti-phosphorylated CREB antibody in the sham group. These findings indicated that the expression as well as phosphorylation of CREB protein was significantly activated in the regions surrounding the infarct area. Since phosphorylation of CREB has recently been implicated in signal transductions that promote the survival and differentiation of neurons, the present data suggest that tissue repair mechanisms may be markedly activated in the pen-infarct area.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (34 results)

All Other

All Publications (34 results)

  • [Publications] KORTARO TANAKA et al.: "Flow threshold for reduction of cyclic AMP biording in the hippocampus CAI and other brain repions during stroke development." J.Cereb.Blood Flow Metab.16. 468-473 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] KORTARO TANAKA et al.: "Calcineurin inhibitor, FK506, prevents reduction in the binding capacity of cylicAMP-dependent protein kinase in ischemao gerbil brain." J.Cereb.Blood Flow Metab.17. 412-420 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] KORTARO TANAKA et al.: "Acute ischemic vulnerability of PKA in the dendintic cabfield of the hippocampus CAI." Neuro Report. 8. 2423-2428 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] KORTARO TANAKA et al.: "Immunohistochemical detection of nitrotyrosine in postischemic cerebral cortex in gerbils." Neurosci.Lett. 235. 85-88 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] KORTARO TANAKA et al.: "Immunohistochemical analysis of cyclic AMP response element binding protein phosphorylation in foal cerebral schemia in rats." Brain Res.818. 520-526 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Shigeaki Suzuki et al.: "Cerebral neurons express interleukin-6 after transient forebrain ischemia in gerbils." Neurosci.Lett.262. 117-120 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] KORTARO TANAKA et al.: "Maturation Phenomenon in Cerebral Ischemia III" Springer-Verlag Berlin, 213 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanaka K.et al.: "Flow threshold for reduction of cyclic AMP binding in the hippocampus CA1 and other brain regions during stroke development in gerbils." J.Cereb.Blood Flow Metab.16. 468-474 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nozaki H.et al.: "Alteration of ryanodine receptor in the hippocampus CA1 after hemispheric cerebral ischemia." Neurochem.Res.21. 975-982 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanaka K.et al.: "Calcineurin inhibitor, FK506, prevents reduction in the binding capacity of cyclic AMP-dependent protein kinase in ischemic gerbil brain." J.Cereb.Blood Flow Metab.17. 412-420 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanaka K.et al.: "Acute ischemic vulnerability of PKA in the Dendritic subfield of the hippocampus CA1." NeuroReport. 8. 2423-2428 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanaka K.et al.: "Immunohistochemical detection of nitrotyrosine in postischemic cerebral cortex in gerbils" Neurosci.Lett.235. 85-88 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Nozaki H.et al.: "Binding capacity of FK506 binding protein after 2-hour hemispheric ischemia in gerbil brain." Brain Res.781. 1-7 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tanaka K.et al.: "Immunohistochemical analysis of cyclic AMP response element binding protein phosphorylation in focal cerebral ischemia in rats." Brain Res.818. 520-526 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Suzuki S.et al.: "Cerebral neurons express interleukin-6 after transient forebrain ischemia in gerbils." Neurosci.Lett.262. 117-120 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] KORTARO TANAKA et al.: "Celcinourin inhibitor FK506 prevents reduction in the birdny capacity of cyclir AMP-lependent protein kinase in ischemic aerbil brain" Journal of Cerebral Blood Flow and Metabolism. 17・4. 412-420 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] 野崎博之,田中耕太郎ら: "砂ネズミ一側総頚動脈結紮モデルにおける小脳半球血流量の経時的検討" 脈管学. 37・5. 247-255 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] HIROYUKI NOZAKI et al.: "Rapid reduction in ryanodine binding of hippocampus CAI in cerebral ischemia" Keio Journal of Medicine. 46・2. 85-89 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] KORTARO TANAKA et al.: "Acute ischemic vulnerability of PKA in the dendsitic cubfielde of the hippocampus CAI" Neuroreport. 8・11. 2423-2428 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] KORTARO TANAKA et al.: "Immunohistochemical detection of nitrotyrosine in postischemic cersical cortor in gerbil" Neuroscience Letters. 235・1. 85-88 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] HIROYUKI NOZAKI et al.: "Binding capacity of Fk506 binding protein after 2-homhemispheric ischemia in gerbil bram" Brain Research. 781・1. 1-7 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] KORTARO TANAKA et al.: "Maturation Phenomenon in Cerebral Ischemia III" Springer-Verlag Berlin, 213 (1999)

    • Related Report
      1998 Annual Research Report
  • [Publications] KORTARO TANAKA et al.: "Calcineurin inhibitor FK506 prevents reduction in the binding capacity of cyclic AMP-dependent protein Knase in i chemic herbil brain." Journal of Cerebral Blood Flow and Metabolism. 17・4. 412-420 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 野崎博之,田中耕太郎ら: "砂ネズミ一側総頚動脈結紮モデルにおける小脳半球血流量の経時的検討" 脈管学. 37・5. 247-255 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] HIROYUKI NOZAKI et al.: "Rapid reduction in ryanodine binding of hippocompus CAI in cerehral ischemia." Keio Journal of Medicine. 46・2. 85-89 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] KORTARO TANAKA et al.: "Acute ischemic vulnerability of PKA in the dendritic subfielclc of the hippocampus CAI." Neuroreport. 8・11. 2423-2428 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] KORTARO TANAKA et al.: "Fmmunohistochemical detection of nitrofyrosine in postischemic cerebral cortox in gerbil." Neuroscience Letters. 235・1. 85-88 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] HIROYUKI NOZAKI et al.: "Binding capacity of FK506 binding protein after 2-hours hemispheric iscliemia in gerhil brain." Brain Research. 781・1. 1-7 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] KORTARO TANAKA et al.: "Flow threshold for reduction of cyclic AMP binding in the hippocampos CA1 and other brain recions during stroke development." Journal of Cerebral Blood Flow and Metabolism. 16・3. 468-473 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] KORTARO TANAKA et al.: "Is nitric oxide really important for regulation of the cerebral circulation" Keio Journal of Medicine. 45・1. 14-27 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Hiroyuki Nozaki et al.: "Alteration of ryanodine receptor in the hippocampus CA1 oftr hemispberic cerehral ischemia." Neurochemical Research. 21・8. 975-982 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] Mineo Matsumoto et al.: "Ataxia and lpileptic seizures in mice lacking type 1 imoitol 1,4,5-tnsphosphate recepton." Nature. 379・6561. 168-171 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 田中耕太郎: "脳虚血と一酸化窒素" 神経研究の進歩. 40・4. 635-654 (1996)

    • Related Report
      1996 Annual Research Report
  • [Publications] 田中耕太郎: "虚血とNO-基礎的側面" 脳と循環. 2・1. 70-75 (1997)

    • Related Report
      1996 Annual Research Report

URL: 

Published: 1996-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi