| Project/Area Number |
08670821
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Jichi Medical School |
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Principal Investigator |
IKEDA Uichi Jichi Medical School, Faculty of Medicine, DEPARTMENT OF CARDIOLOGY,Associate Professor, 医学部, 助教授 (30221063)
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| Project Period (FY) |
1996 – 1997
|
| Project Status |
Completed (Fiscal Year 1997)
|
| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1997: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | gene therapy / nitric oxide / hypertension / 心肥大 / 遺伝子導入 |
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| Research Abstract |
(1).The inducible NO synthase (iNOS) exists in cardiac myocytes and vascular smooth muscle cells. We revealed that neurohumoral factors modulate iNOS expression in these cells. For example, protein kinase C activating agents such as angiotensin II and vasopressin augment cytokine-induced iNOS expression in cardiac myocytes. cAMP-generating neurohumoral factors adrenomedullin and isoproterenol also augment NO production by cytokine-stimulated cardiac myocytes.
(2).We investigated whether cardiac myocytes and vascular smooth muscle cells could be efficiently transduced with adeno-associated virus (AAV) vectors, and found their efficient transfer into these cells. These observations suggest that AAV vectors are promising for cardiac gene therapy.
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