Megakaryocytic differentiation of hematopoietic stem cell by NF-E2 transcription factors.
| Project/Area Number |
08670847
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Hirosake University |
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Principal Investigator |
ITO Etsuro Hirosaki University, University Hospital Assistant Professor, 医学部附属病院, 講師 (20168339)
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| Co-Investigator(Kenkyū-buntansha) |
TOKI Tsutomu Hirosaki University, School of Medicine Instructor, 医学部, 助手 (50195731)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 1997: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1996: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | megakaryocyte / p45 NF-E2 / Nfr1 / Nrf2 / mafK / mafG |
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| Research Abstract |
NF-E2 is a heterodimer composed of a lineage-specific subunit p45 and a ubiquitous subunit p18 which belongs to small Maf family. In order to understand the role of NF-E2 transcription factors in megakaryocytic differention of hematopoietic stem cells, we have studied the expression of the transcription factors in purified early hematopoietic progenitor cells (HPCs) induced to unilineage megakaryocytic differentiation in a liquid culture system by reverse transcriptase mediated polymerase chain reaction (RT-PCR). Expression of p45 NF-E2 and its related transcription factors, Nrf1 and Nrf2, was induced during megakaryocytic differentiation. Furthermore, we found that p18 (mafK) and mafG were also induced during megakaryocytic differention using chronic myelogenous leukemia (CML) blasts. Especially expression of mafG was induced more abundantly. These results indicated that Nrf1 and Nrf2 as well as p45 play an important role in megakaryocytic differentiation as heterodimers with small Mafs.
We next studied the possibility that Nrf1 and Nrf2 from heterodimers with small Mafs. We construct prokaryotic expression plasmids for mafK,mafG,p45, Nrf1 and Nrf2. Recombinant proteins were purified with Ni-NTA regin and Electrophoretic gel mobility shift assays (EGMSA) was performed. EGMSA showed that small Maf proteins form heterodimers with Nrf1 and Nrf2 and recognize the NF-E2 motif.
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Report
(3 results)
Research Products
(9 results)
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[Publications] Onodera K,Yomogida K,Suwabe N,Takahasi S,Muraosa Y,Hayashi N,Ito E,Gu L,Rassoulzadegan M,Engel JD and Yamamoto M.: "Conserved structure, regulatory elements, and transcriptional regulation from the GATA-1 gene testis promoter." J Biochem.121. 251-263 (1997)
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[Publications] Hirai H,Shimazaki C,Yamagata N,Goto H,Inaba T,Kikuta T,Sumikuma T,Sudo Y,Ashihara E,Fujita N,Hibi S,Imashuku S,Ito E,Nakagawa M.: "Effects of thrombopoietin (c-mpl ligand) on growth ofblast cells from patients with transient abnormal myelopoiesis and acute myeloblastic leukemia." Eur J Haematol. 59. 38-46 (1997)
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