| Project/Area Number |
08670852
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Tohoku University |
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Principal Investigator |
MINEGISHI Masayoshi Tohoku University, Institute of Development, Aging and Cancer, Instructor, 加齢医学研究所, 助手 (20211592)
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| Co-Investigator(Kenkyū-buntansha) |
TUCHIYA Shigeru Tohoku University, Institute of Development, Aging and Cancer, Associate Profess, 加齢医学研究所, 助教授 (30124605)
KONNO Tasuke Tohoku University, Institute of Development, Aging and Cancer, Professor, 加齢医学研究所, 教授 (00004846)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | Neuroblastoma / Cell line / Adhesion molecule / Integrins / Signal Transduction / アポトーシス / インターフェロンα |
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| Research Abstract |
We have established a novel neuroblastoma cell line, of which the growth is dependent on the attachment to extracellular matrix (ECM). Electron microscopic study revealed that the established cells (N-KAS) had neurosecretory granules with halo. Slot blot analysis revealed 52 copies of N-myc gene status.These results confirm the neuroblastic nature of the cells. Attachment to either fibronectin, Iaminin, or type IV collagen supported the growth of N-KAS cells, although that to poly-L-lysine did not. Moreover, when anti-CD29 (beta1-integrin) antibody 4B4 was added into the culture, attachment and growth of the cells were inhibited in these ECM-coated dishes. The enhancement of p130^<Cas> tyrosine phoshorylation was detected in the N-KAS cells growing in fibronectin, laminin, and type IV collagen-coated dishes. These findings indicate that stimulatory signals through beta1-integrins might play an important role in the proliferation of N-KAS cells on ECM.
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