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Telomerase Activity of the Blood Cells in Patients with Myeloproliferative Disorders in Childhood

Research Project

Project/Area Number 08670891
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

NISHIMURA Shinichiro  Hiroshima University Medical Hospital Research Associate, 医学部・附属病院, 助手 (00228222)

Co-Investigator(Kenkyū-buntansha) TASHIRO Satoshi  Hiroshima University School of Medicine Research Associate, 医学部, 助手 (20243610)
HIYAMA Eisou  Hiroshima University Medical Hospital Assistant Professor, 医学部・附属病院, 講師 (00218744)
UEDA Kazuhiro  Hiroshima University School of Medicine Professor, 医学部, 教授 (30112189)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥1,300,000 (Direct Cost: ¥1,300,000)
Keywordstelomere / telomerase / Myeloproliferative Disorders in Childhood
Research Abstract

It is not known exactly whether myeloproliferative disorders ; MD in childhood (juvenile chronic myelogenous leukemia ; JCML,transient myeloproliferative disorder ; TMD) are ture malignacy or not. To address this question, we examined telomerase expression of the blood cells in patients with MD.Telomerase activity was found in all leukemic cells of 10 patients with acute lymphoblastic leukemia ; ALL.Terminal restriction fragment ; TRF lengths in the leukemic cells were shorter than that in normal blood cells, although it was not significant. In two patients with JCML and one with TMD,telomerase activity was also found, but TRF lengths in the blood cells were within 8kb to 15kb consist with those of blood cells from age-matched normal donors. Because normal lymphocytes and hematopoietic progenitor cell express telomerase activity, further investigation is needed to clarify the relationship between hematological malignacy and telomerase activiry.
In the next experiment, we examined the proportion of delected mitochondrial DNA in blood cells from three patients with Peason syndrome. Southern blot analysis represented that the proportion of deleted mitochondorial DNA in peripheral blood and bone marrow has a tendency to correlate to the severity of hematological manifestation.
We will study the relationship between mitochondorial abnormality and telomerase acticity in blood cells from patients with MD in future.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] 西村 真一郎: "乳児白血病" 小児内科. 28. 1091-1095 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K. Muraki et al.: "The association between haematological manifestation and mtDNA in Pearson syndrome" J. Inher. Metab. Dis. 20. 697-703 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shinichiro Nishimura: "Infant Leukemia" Jap.J.Pediatr.Med.28. 1091-1095 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Muraki: "The association between haematological manifestation and mtDNA deletions in Pearson syndrome." J.Inher.Metab.Dis.20. 697-703 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] K.Muraki et al.: "The association between hoematological manifestation and mtDNA deletious in Pearson syndrome" J.Inher.Metab.Dis.20. 697-703 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 西村真一郎: "乳児白血病" 小児内科. 28(増刊号). 1091-1095 (1996)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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