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IDENTIFICATION OF NEW GLOMERULAR PROGRESSION FACTORS USING MONOCLONAL ANTIBODY

Research Project

Project/Area Number 08670894
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionThe University of Tokushima

Principal Investigator

KAGAMI Shoji  SCHOOL OF MEDICINE,The University of Tokushima ASSISTANT PROFESSOR, 医学部, 講師 (00224337)

Co-Investigator(Kenkyū-buntansha) KIDO Hiroshi  INSTITUTE OF ENZYME RESEARCH,The University of Tokushima PROFESSOR, 酵素科学研究センター, 教授 (50144978)
KURODA Yasuhiro  SCHOOL OF MEDICINE,The University of Tokushima PROFESSOR, 医学部, 教授 (20035471)
Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 1997: ¥200,000 (Direct Cost: ¥200,000)
Fiscal Year 1996: ¥2,000,000 (Direct Cost: ¥2,000,000)
Keywordsmonoclonal antibody / 1F3 / Integrin / progression factors in GN
Research Abstract

In order to identify a new cell surface antigen as a potential marker of renal glomerular cell injury, we produced a monoclonal antibody (Mab), 1F3, by immunizing mice with cultured human glomerular cells. Immunohistochemical studies demonstraated that 1F3 recognizing molecule (1F3 molecule) was strongly expressed on the cell surface of glomerular podocytes and very weakly on parietal epithelial cells in the normal kidney. Immunoprecipitation revealed that 1F3 recognized a 125 KDa protein under reducing conditions. Sequenced N-terminal amino acid analysis following 1F3 immunoaffinity purification indicated that 1F3 molecule resembled integrin alpha3 subunit. Biopsy specimens from patients with a variety of glomerular and tubulointerstitial diseases showed that 1F3 antigen was almost negative in cellular crescents but was strongly expressed in fibro-cellular crescents. 1F3 antigen became positive in atrophic tubules that were seen in diseased kidneys. Severity of tubular atrophy correlated well with the extent of tubular expression of 1F3 antigen. Urine examination using ELISA showed that increased excretion of 1F3 molecule was observed in the patients with glomerulonephritis as compared with healthy donor. These results indicate that Mab, 1F3 marks cell biological changes of renal epithelial cells under disease conditions and may be a useful marker for progressive kidney diseases.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (5 results)

All Other

All Publications (5 results)

  • [Publications] 森本 雄次: "ヒト糸球体上皮細胞に対すモノクロナール抗体1F3:腎上皮細胞障害の新しいマーカー" 日本小児腎臓病学会雑誌. 9. 88-94 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shoji Kagami: "A monoclonal antibody(1F3)to human glomerular eqithelial cells:A new marker to renal epithelial cell injury" Nephron. 75(1). 65-71 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Morimoto Y et al.: "A monoclonal antibody against human epithelial cells (in japanese)" Jap.J.pediat.Nephrol.88. 222-228 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Kagami S et al.: "A monoclonal Antibody (1F3) to Human Glomerular Epithelial Cells : A new Marker for Renal Epithelial Cell Injury" Nephron. 75. 65-71 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Shoji Kagami: "A monoclonal antibody(1F3)to human glomerular epithelial cells:A new marker to renal epithelial cell injury" Nephron. 75(1). 65-71 (1997)

    • Related Report
      1996 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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