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Establishment of purified blood CD34+ cell transplantation from related mismatched donors

Research Project

Project/Area Number 08670896
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionThe University of Tokushima

Principal Investigator

KAWANO Yoshifumi  University Hospital of Tokushima, Department of Pediatrics, Assistant Pediatrician, 医学部・附属病院, 助手 (20260680)

Project Period (FY) 1996 – 1997
Project Status Completed (Fiscal Year 1997)
Budget Amount *help
¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1997: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1996: ¥1,200,000 (Direct Cost: ¥1,200,000)
KeywordsCD34+ cell / GVHD / mismatch / DLI / Stem cell transplantation / HLA部分一致 / 末梢血幹細胞移植術
Research Abstract

One major limitation of allogeneic bone marrow transplantation (BMT) is the lack of suitable donors. The results of transplantation with unrelated marrow grafts are, in general, inferior to those using matched sibling marrow, with an increased incidence and severity of graft-versus-host disease (GVHD), graft rejection and infections, although new immunosuppressive therapies have made the prophylaxis or treatment of GVHD more effective. Mobilized allogeneic peripheral blood cells (PBC) have recently been shown to be a potential source of stem cells for hematopoietic reconstitution after myeloablative therapy. Most importantly, more hematopoietic cells can be collected from blood than from bone marrow or cord/placenta. In a previous study, we demonstrated that negative depletion of T cells in unsatisfactory with PBC grafts, and is associated with a significant loss of hematopoietic progenitor cells. On the other hand, recently developed techniques for the positive selection/enrichment of CD34+ cells from marrow or PBC provide a convenient method for concentrating hematopoietic progenitors and depleting T cells. From this phase I clinical trial with 13 pediatric patients, we found that GVHD remained problematic after transplantation with enriched blood CD34+ cells, and no dose-dependent effect of T cells was seen for the induction of acute GVHD.The major infectious complication was cytomegalovirus infection, which was successfully managed with gamma-globulin and gancyclovir treatment with or without additional DLI.In conclusion, our results show that enriched blood CD34+ cells from a mismatched allogeneic donor can be a realistic alternative source of stem cells in patients who are at immediate risk of high-risk disease. The therapeutic efficacy and safety of periodic DLI as an integrated part of the protocol needs to be clarified in a further study.

Report

(3 results)
  • 1997 Annual Research Report   Final Research Report Summary
  • 1996 Annual Research Report
  • Research Products

    (18 results)

All Other

All Publications (18 results)

  • [Publications] Yoshifumi Kawanao, et, al.: "Partially mismatched pediatric transplants with allogeneic CD34+blood cells from a related donor." Blood. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T Kajiume, Y Kawano, et.al.: "New consecutive high-dose chemotherapy modality with fractionated blood stem cell support in the treatment of high-risk pediatric solid tumors:a feasibility syudy." Bone Marrow Transplant. 21. 147-151 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hyeoug Joon Kim, et.al.: "Evaluation of a cytokine combination inducing thronbopoietin for improvcd transduction of a retroviral gene into G-CSF-mobilized CD34+human blood cells." STEM CELLS. 15. 347-352 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takanori Abe, Yoshifumi Kawano,: "Transduction of retrovirus-mediated NeoR gene into CD34+cells purified from granulocyte colony-stimulating fadctr(G-CSF)-mobilized infant and cord bolld." Experimental Hematology. 25. 966-971 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Ysuhiro Okamoto, Yoshifumi kawano,: "Serum Thrombopoietin levels in patients receiving high-dose chemotherapy with support of purified peripheral blood CD34+cells." Cancer Research. 57. 5037-5040 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takanori Abe, Yoshifumi Kawano,: "Effects of recombinant erythropoietin in interaction with stromal fators on cord blood hematopoiesis." Blood. 87. 3212-3217 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshifumi Kawano: "Partially mismatched pediatric transplants with allogeneic CD34+ blood cells from a related donor." accepted for publication Blood.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] T Kajiume: "New consecutive high-dose chemotherapy modality with fractionated blood stem cell support in the treatment of high-risk pediatric solid tumors : a feasibility study." Bone Marrow Transplant. 21. 147-151 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Hyeoug Joon Kim: "Evaluation of a cytokine combination inducing thrombopoietin for improved transduction of a retroviral gene into G-CSF-mobilized CD34+ human blood cells." Stem Cells. 15. 347-352 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takanori Abe: "Transduction of retrovirus-mediated NeoR gene into CD34+ cells purified from granulocyte colony-stimulating factor (G-CSF) -mobilized infant and cord blood." Exp Hematol. 25. 966-971 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yasuhiro Okamoto: "Serum Thrombopoietin levels in patients receiving high-dose chemotherapy with support of purified periheral blood CD34+ cells." Cancer Research. 57. 5037-5040 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Takanori Abe: "Effects of recombinant erythropoietin in interaction with stromal factors on cord blood hematopoiesis." Blood. 87. 3212-3217 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1997 Final Research Report Summary
  • [Publications] Yoshifumi Kawnao, et.al.: "Partially mismatched pediatric transplants with allogeneic CD34+blood cells from a related donor." Blood. (in press).

    • Related Report
      1997 Annual Research Report
  • [Publications] T.Kajiume, Y Kawano, et.al.: "New consecutive high-dose chemotherapy modality with fractionated blood stem cell support in the treatment of high-risk pediatric solid tumors:a feasibility study." Bone Marrow Transplant. 21. 147-151 (1998)

    • Related Report
      1997 Annual Research Report
  • [Publications] Hyeoug Joon Kim, et.al.: "Evaluation of a cytokine combination inducing thronbopoietin for improved transduction of a retroviral gene into G-CSF-mobilized CD34+ human blood cells." STEM CELLS. 15. 347-352 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takanori Abe, Yoshifumi Kawano,: "Transduction of retrovirus-mediated NeoR gene into CD34+ cells purified from granulocyte colony-stimulating factor(G-CSF)-mobilazed infant and cord blood." Experimental Hematology. 25. 966-971 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Ysuhiro Okamoto, Yoshifumi Kawano,: "Serum Thrombopoietin levels in patients receiving high-dose chemotherapy with support of purified peripheral blood CD34+ cells." Cancer Research. 57. 5037-5040 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] Takanori Abe, Yoshifumi Kawano,: "Effects of recombinant erythropoietin in interaction with stromal fators on cord blood hematopoiesis." Blood. 87. 3212-3217 (1996)

    • Related Report
      1997 Annual Research Report

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Published: 1996-04-01   Modified: 2016-04-21  

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