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The investigation of role and localization of glucose transporters (GLUT) in epidermal keratinocytes

Research Project

Project/Area Number 08670939
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionAsahikawa Medical College

Principal Investigator

HASHIMOTO Yoshio  Dep.of Dermatology, Associate Professor, 医学部, 助教授 (70172880)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Satoshi  Dep.of Dermatology, Assistant Professor, 医学部, 助手 (20292112)
TAKAHASHI Hidetoshi  Dep.of Dermatology, Assistant Professor, 医学部, 講師 (00216748)
田村 俊哉  旭川医科大学, 医学部, 助手 (30217195)
Project Period (FY) 1996 – 1998
Project Status Completed (Fiscal Year 1998)
Budget Amount *help
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1998: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1997: ¥600,000 (Direct Cost: ¥600,000)
Keywordsglucose / glucose transporter / GLUT / keratinocyte / facilitative diffusion / tape stripping / UVB / GLUT1 / GLUT 1 / Tape-Stripping / UVB照射 / TPA処理 / 尋常性乾癬
Research Abstract

The transport of glucose across the plasma membrane of mammalian cells represents one of the most important cellular nutrient transport events, since glucose plays a central role in cellular homeostasis and metabolism. It has long been established that the plasma membranes of virtually all mammalian cells processes a transport system for glucose of the facilitative diffusion type ; these glucose transporters allows the movement of glucose across the plasma membrane down its chemical gradient either into or out of cells. These transporters include six types, the GLUT1/erythrocyte, GLUT2/liver, GLUT3/brain, GLUT4/muscle-fat, GLUT5/small intestine, and GLUT7/liver microsome. The extent and significance of GLUT expression in human epidermis is largely unknown. Pig epidermis has been suggested to be an appropriate substitute for the human epidermis because of the close similarity in architecture. This study was undertaken to determine the expression of GLUT in pig epidermal keratinocytes and hyperproliferative keratinocytes. We demonstrate, for the first time, that GLUT1 is highly expressed in normal pig skin and that GLUT1 protein is localized in the basal and the immediately suprabasal layer of the epidermis by immunohistochemistry. In addition, GLUT1 protein (45KD) is detected in pig epidermal extracts by Western-blotting. UVB-induced hyperproliferative epidermis showed enhanced GLUT1 expression. The increase in expression of GLUT1 protein were also determined in tape stripping-induced heperproliferative epidermis. No reactivity for GLUT2-GLUT5 was observed in pig epidermal keratinocytes by immunohistochemistry and Western-blotting. Our data suggested that GLUT1 is highly expressed in the basal layers of pig epidermis and that its expression is modulated by keratinocyte proriferation.

Report

(4 results)
  • 1998 Annual Research Report   Final Research Report Summary
  • 1997 Annual Research Report
  • 1996 Annual Research Report
  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] 橋本喜夫、川岸尚子、他: "選択的PKC阻害剤カルフォスチンCの豚表皮細胞動態に与える影響" Cytometry Research. 6. 9-17 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 橋本喜夫、田村俊哉、他: "豚表皮DNA合成とβアドレナリンアデニル酸シクラーゼ反応性に与える各種漢方生薬、方剤の作用" 日本皮膚科学会誌. 106. 1083-1089 (1996)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ishida-Yamamoto A, Hashimoto Y, et al: "Distinctive expression of filaggrin and trichohyalin during various pathways of epithelial differentiation." British Journal of Dermatology. 137. 9-16 (1997)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takahashi H, Hashimoto Y, et al: "Interferon-γ-dependent induction of manganese superoxide dismutase activity of SV40-transformed human keratinocytes by anti-Fas antibody and by TNF-α" Journal of Dermatologycal Science. 16. 191-199 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tamura T, Hashimoto Y, et al: "Functional alteration of guanine nucleotide binding proteins (Gs and Gi) in psoriatic epidermis" Journal of Dermatological Science. 17. 61-68 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] kawagishi N, Hashimoto Y, et al: "Epidermal cell kinetics of pig skin in vivo following UVB irradiation : apoptosis induced by UVB is enhanced in hyperproliferative skin condition," Journal of Dermatological Science. 18. 43-53 (1998)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hashimoto Y,Kawagishi N,Tamura T,Iizuka H: "Effects of the selective protein kinase C inhibitor, calphostin C,on pig epidermal keratinocytes : thymidine incorporation and flow cytometric analyses." Cytometry Research. 6. 9-17 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Hashimoto Y,Tamura T,Takahashi H,Iizuka H: "Effects of various Kampo medicines on DNA synthesis and beta-adrenergic adenylate cyclase responses of pig epidermis." Jpn J Dermatol. 106. 1083-1089 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toyota N,Hashimoto Y,Matsuo S,Kitamura Y,Iizuka H: "Effects of FK506 and cyclosporin A on proliferation, histamine release and phenotype of murine mast cells." Arch Dermatol Res. 288. 474-480 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Toyota N,Sakai H,Takahashi H,Hashimoto Y,Iizuka H: "Inhibitory effect of 1alpha, 25-dihydroxyvitamin D3 on mast cell proliferation and A23187-induced histamine release, also accompanied by a decreased c-kit receptor" Arch Dermatol Res. 288. 709-715 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Ishida-Yamamoto A,Hashimoto Y,Manabe M,O'guin WM,Dale BA,Iizuka H: "Distinctive expression of filaggrin and trichohyalin during various pathways of epithelial differentiation" Br Journal of Dermatology. 137. 9-16 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Takahashi H,Hashimoto Y,Kinouchi M,Iizuka H: "Interferon-gamma-dependent induction of manganese superoxide dismutase activity of SV40-transformed human keratinocytes by anti-Fas antibody and by TNF-alpha" Journal of Dermatological Sci. 16. 191-199 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Tamura T,Takahashi H,Ishida-Yamamoto A,Hashimoto Y,Iizuka H: "Functional alteration of guanine nucleotide binding proteins (Gs and Gi) in psoriatic epidermis" Journal of Dermatol Sci. 17. 61-68 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] Kawagishi N,Hashimoto Y,Takahashi H,Ishida-Yamamoto A,Iizuka H: "Epidermal cell kinetics of pig skin in vivo following UVB irradiation : apoptosis induced by UVB is enhanced in hyperproliferative skin condition" Journal of Dermatological Sci. 18. 43-53 (1998)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      1998 Final Research Report Summary
  • [Publications] 橋本喜夫、川岸尚子、他: "選択的PKC阻害剤カルフォスチンCの豚表皮細胞動態に与える影響" Cytometry Research. 6. 9-17 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] 橋本喜夫、田村俊哉、他: "豚表皮DNA合成とβアドレナリンアデニル酸シクラーゼ反応性に与える各種漢方生薬、方剤の作用" 日本皮膚科学会誌. 106. 1083-1089 (1996)

    • Related Report
      1998 Annual Research Report
  • [Publications] Ishida-Yamamoto A,Hashimoto Y,et al: "Distinctive expression of filaggrin and trichohyalin during various pathways of epithelial differentiation." British Journal of Dermatology. 137. 9-16 (1997)

    • Related Report
      1998 Annual Research Report
  • [Publications] Takahashi H,Hashimoto Y,et al: "Interferon-γ-dependent induction of manganese superoxide dismutase activity of SV40-transformed human keratinocytes by anti-Fas antibody and by TNF-α" Journal of Dermatological Science. 16. 191-199 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Tamura T,Hashimoto Y,et al: "Functional alteration of guanine nucleotide binding proteins(Gs and Gi)in psoriatic epidermis" Journal of Dermatological Science. 17. 61-68 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] Kawagishi N,Hashimoto Y,et al: "Epidermal cell kinetics of pig skin in vivo following UVB irradiation : apoptosis induced by UVB is enhanced in hyperproliferative skin condition." Journal of Dermatological Science. 18. 43-53 (1998)

    • Related Report
      1998 Annual Research Report
  • [Publications] A.Ishida-Yamamoto, et al: "Distinctive expression of filaggrin and trichohyalin during various pathways of epithelial differentiation" British Journal of Dermatology. 137. 9-16 (1997)

    • Related Report
      1997 Annual Research Report
  • [Publications] 橋本 喜夫: "豚皮膚器官培養系に与える各種生薬相互作用の検討" 漢方医学. 22. 16-18 (1998)

    • Related Report
      1997 Annual Research Report

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Published: 1997-04-01   Modified: 2016-04-21  

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