The expression of extracellular matrix-related genes in skin cancers
| Project/Area Number |
08670947
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Chiba University |
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Principal Investigator |
TSUKIFUJI Reiko Chiba University School of Medicine, Lecturer, 医学部・付属病院, 講師 (70218048)
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| Co-Investigator(Kenkyū-buntansha) |
HATAMOCHI Atsushi Chiba University School of Medicine, Associated Professor, 医学部, 助教授 (90172923)
SHINKAI Hiroshi Chiba University School of Medicine, Professor, 医学部, 教授 (90030957)
坂井 靖夫 千葉大学, 医学部・附属病院, 助手 (50272315)
黒田 啓 千葉大学, 医学部, 助手 (90225300)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1997: ¥500,000 (Direct Cost: ¥500,000)
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| Keywords | skin cancer / extracellular matrix / matrix metalloproteinase / actinic keratosis / glycosaminoglycans |
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| Research Abstract |
1.We have reported that digestion of glycosaminoglycans by chondroitin ABC lyase enhance hybirdization signals in in situ hybridization techniques. (Histochemical Journal, 1997)
2. Gene expression of matrix metalloproteinase (MMP) -1, -2 and -3 was observed in the stromal cell of basal cell carcinoma (BCC) with tumor cell nests in the dermis. But no expression was found in the superficial type of (BCC).
3.MMP-1 mRNA was detected in tumor cells and/or in stromal cells in all cases of squamous (SCC) cell carcinoma, 4 of 6 actinic kertosis (AK) adjacent to SCC and 4 of 16 AKs. MMP-2 and MMP-3 mRNAs were detected in SCC but not in AK.
4.The expression of MMP-3 was correlated with that of MMP-1 localized at the tumor mass and stroma of the invasive area, while MMP-2 mRNA was detcted widely throughout the stroma independent of MMP-1 expression in SCC.
5.Our results indicated that the expression of MMP 1, -2 and -3 showed different localization patterns, suggesting a unique role of each MMP in tumor progression. Moreover, MMP-1 expression could be an early event in the development of SCC,and AK demonstrating MMP-1 mRNA might be in a more advanced dysplastic state, progressing to SCC.
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Report
(3 results)
Research Products
(7 results)
