| Project/Area Number |
08670954
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Niigata University |
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Principal Investigator |
KAWAI Kazuhiro Niigata University School of Medicine, Department of Dermatology, Research Associate, 医学部, 助手 (90242411)
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| Project Period (FY) |
1996 – 1997
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| Project Status |
Completed (Fiscal Year 1997)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 1997: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1996: ¥1,100,000 (Direct Cost: ¥1,100,000)
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| Keywords | hu-PBL-SCID mice / cultured epidermis / skin allograft |
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| Research Abstract |
To determine whether Langerhans cell-free cultured human epidermal grafts survive for a prolonged period in allogeneic recipients, an experimental model using SCID mice reconstituted with human peripheral blood lymphocytes (hu-PBL) was established. In this model, hu-PBL were activated with anti-CD3 mAb in vitro and then transferred into SCID mice pretreated with anti-asialoGM1 Ab in vivo. Flow cytometry of the peripheral lymphoid organs demonstrated engraftment of hu-PBL (mainly T cells) 2-4 weeks after the transfer. Cultured human epidermal grafts were accepted by untreated by untreated SCID mice, when transplanted on the dorsal muscle and protected with a silicon transplantation chamber. Ten days after cultured human epidermal grafting on SCID mice reconstituted with allongeneic hu-PBL,however, detachment of the epidermis and infiltrantion of human CD3+ T cells in the graft bed were histologically confirmed. These results suggest that mice. This model will be usefull for analysis of the rejection mechanisms of cultured human epidermal allonrafts.
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